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Shoot bud regeneration from different explants of Bacopa monniera (L.) Wettst. by trimethoprim and bavistin

  • Cell Biology and Morphogenesis
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Abstract

A mass in vitro propagation system devoid of growth regulators for Bacopa monniera (L.) Wettst., a traditional Indian medicinal plant, has been developed. Direct shoot bud regeneration was induced by culturing internode and leaf explants on Murashige and Skoog's (MS) medium supplemented with an antibiotic (trimethoprim) or a fungicide (bavistin). Bavistin showed a marked cytokinin-like activity, as evident from high number of shoot buds induced in node, internode and leaf explants. Optimum adventitious shoot buds induction occurred at 300 mg/l bavistin from internode explants. In vitro regenerated shoots were elongated and rooted before transferred to field with 85% survival. The regeneration protocol developed in this study illustrates the usefulness of additives for mass propagation and germplasm conservation of B. monniera.

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Abbreviations

BA:

6-Benzyladenine

BVN:

Bavistin {methyl benzimidozole carbamate}

GR:

Growth regulator

IAA:

Indole-3-acectic acid

IBA:

Indole-3-butyric acid

Kn:

Kinetin

MS medium:

Murashige and Skoog (1962) medium

NAA:

α-Naphthaleneacetic acid

TDZ:

Thidiazuron (N-phenyl-N′-1,2,3-thidiazol-5-ylurea)

TMP:

Trimethoprim{2,4 di-amino-5-{3,4,5-trimethoxybenzyl}pyrimidine

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Acknowledgements

The financial support from University Grant Commission (UGC) (New Delhi, India) to V.T. is gratefully acknowledged. The work is dedicated in the memory of Late Parvati Tiwari.

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Author notes

  1. Vaibhav Tiwari

    Present address: Ocular Virology Lab, Department of Ophthalmology-Visual Sciences, LIERI Building, University of Illinois at Chicago, Chicago, IL, 60612, USA

Authors and Affiliations

  1. Faculty of Sciences, School of Biotechnology, Banaras Hindu University, Varanasi, 221005, UP, India

    Vaibhav Tiwari & Brahma Deo Singh

  2. Department of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, 221005, UP, India

    Kavindra Nath Tiwari

Authors
  1. Vaibhav Tiwari
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  2. Kavindra Nath Tiwari
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  3. Brahma Deo Singh
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Correspondence to Vaibhav Tiwari.

Additional information

Communicated by H. S. Judelson

Authors Vaibhav Tiwari and Kavindra Nath Tiwari contributed equally to this work

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Tiwari, V., Tiwari, K.N. & Deo Singh, B. Shoot bud regeneration from different explants of Bacopa monniera (L.) Wettst. by trimethoprim and bavistin. Plant Cell Rep 25, 629–635 (2006). https://doi.org/10.1007/s00299-006-0126-5

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  • DOI: https://doi.org/10.1007/s00299-006-0126-5

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