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Cloning and expression analysis of Cs-TIR1/AFB2: the fruit development-related genes of cucumber (Cucumis sativus L.)

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Abstract

Phytohormone auxin plays an important role in fruit development and is perceived by the TIR1/AFB family of F-box proteins as auxin receptors involved in auxin signal pathway. Cucumber (Cucumis sativus L.) fruit development is either parthenocarpic or non-parthenocarpic. However, little is known on TIR1 and AFB participation in the early stage of cucumber fruit development. In present study, TIR1 and AFB2 were isolated from cucumber. CsTIR1 and CsAFB2 were highly expressed in leaves and ovaries. Their transcript levels decreased in parthenocarpic and pollinated fruits, but continuously up-regulated in aborted fruits, indicating that down-regulation of CsTIR1 and CsAFB2 may be in favor of cucumber fruit set and development. The transcript levels of CsTIR1 and CsAFB2 were significantly induced in leaves by NAA, 6-BA, GA3, ABA, and ethephon. The expression levels were up-regulated by ABA and ethephon treatments. This expression patterns was accordant with the aborted fruits. Thus, CsTIR1 and CsAFB2 may be important regulators during cucumber fruit development.

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Abbreviations

CPPU:

N-(2-ehloro-4-Pyidyl)-N′-Phenylurea

TIR1:

Transport inhibitor response 1

AFB:

Auxin signaling F-box

NAA:

a-Naphthalene acetic acid

6-BA:

6-Benzylaminopurine

GA3 :

Gibberellin

ABA:

Abscisic acid

ORF:

Open reading frame

UTR:

Untranslated region

SKP1:

S-Phase kinase-associated protein 1

SAPK7:

Serine/threonine-protein kinase 7

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Acknowledgments

This work was supported by funds from the National Natural Science Foundation of China grant (The 973 Program: 2012CB3904), “Youth Science and Technology Innovation Fund” program of Nanjing agricultural university (No. KJ2012013), “Fundamental Research of Nanjing Agricultural University (Y0201100253)” and “Ph.D. Programs Foundation of Ministry of Education of China (20120097120037)”.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Li Cui, Tinglin Zhang, Ji Li, Qunfeng Lou & Jinfeng Chen

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  1. Li Cui
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  2. Tinglin Zhang
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  3. Ji Li
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  4. Qunfeng Lou
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  5. Jinfeng Chen
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Correspondence to Jinfeng Chen.

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Communicated by Y. Wang.

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Cui, L., Zhang, T., Li, J. et al. Cloning and expression analysis of Cs-TIR1/AFB2: the fruit development-related genes of cucumber (Cucumis sativus L.). Acta Physiol Plant 36, 139–149 (2014). https://doi.org/10.1007/s11738-013-1394-7

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