Skip to main content
SpringerLink
Log in

Characterization of five CIPK genes expressions in maize under water stress

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

ABA, H2O2 and Ca2+ play critical roles as signals in the adaptive responses of plants to water and other stresses. They accumulate in plant cells under water and other stresses and induce changes in stress-related gene expressions. CIPKs, protein kinases associated with a calcineurin B-like calcium sensor, play a role in the regulation of stress gene expression in plants. However, it is still unclear whether ABA and H2O2 are key inducers that regulate the changes in CIPK expressions under water stress. In this study, five stress-inducible CIPKs in maize were retrieved from Database. They were designated as ZmCIPK1, 3, 8, 17 and 18, based on their homologies with known CIPK sequences. The expressions of the five ZmCIPKs in maize leaves and roots were analyzed and found to be regulated by PEG, CaCl2, ABA and H2O2 to different extents. Moreover, the effect of ABA and H2O2 on the expressions of ZmCIPKs under water stress was in an organ-dependent manner.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Cantrel C, Vazquez T, Puyaubert J, Rezé N, Lesch M, Kaiser WM, Dutilleul C, Guillas I, Zachowski A, Baudouin E (2010) Nitric oxide participates in cold-responsive phosphosphingolipid formation and gene expression in Arabidopsis thaliana. New Phytol 189:415–427

    Article  PubMed  Google Scholar 

  • Cheong YH, Pandey GK, Grant JJ, Batistic O, Li L, Kim BG, Lee SC, Kudla J, Luan S (2007) Two calcineurin B-like Ca2+ sensors, interacting with protein kinase CIPK23, regulate leaf transpiration and root potassium uptake in Arabidopsis. Plant J 52:223–239

    Article  PubMed  CAS  Google Scholar 

  • D’Angelo C, Weinl S, Batistic O, Pandey GK, Cheong YH, Schultke S, Albrecht V, Ehlert B, Schulz B, Harter K, Luan S, Bock R, Kudla J (2006) Alternative complex formation of the Ca-regulated protein kinase CIPK1 controls abscisic acid-dependent and independent stress responses in Arabidopsis. Plant J 48:857–872

    Article  PubMed  Google Scholar 

  • Harmon AC, Gribskov M, Harper JF (2000) CDPKs: a kinase for every Ca2 + signal? Trends Plant Sci 5:154–159

    Article  PubMed  CAS  Google Scholar 

  • Hu XL, Zhang AY, Zhang JH, Jiang MY (2006) Abscisic acid is a key inducer of hydrogen peroxide production in leaves of maize plants exposed to water stress. Plant Cell Physiol 47:1484–1495

    Article  PubMed  CAS  Google Scholar 

  • Jaiswal JK (2001) Calcium—how and why? J Biosci 26:357–363

    Article  PubMed  CAS  Google Scholar 

  • Jiang MY, Zhang JH (2001) Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol 42:1265–1273

    Article  PubMed  CAS  Google Scholar 

  • Kawano T, Muto S (2000) Mechanism of peroxidase actions for salicylic acid-induced generation of active oxygen species and an increase in cytosolic Ca2+ in tobacco cell suspension culture. J Exp Bot 51:685–693

    Article  PubMed  CAS  Google Scholar 

  • Kiegle E, Moore CA, Haseloff J, Tester MA, Knight MR (2000) Cell-type-specific calcium responses to drought, salt and cold in the Arabidopsis root. Plant J 23:267–278

    Article  PubMed  CAS  Google Scholar 

  • Kim KN, Cheong YH, Gupta R, Luan S (2000) Interaction specificity of Arabidopsis calcineurin B-like calcium sensors and their target kinases. Plant Physiol 124:1844–1853

    Article  PubMed  CAS  Google Scholar 

  • Kim KN, Cheong YH, Grant JJ, Pandey GK, Luan S (2003a) CIPK3, a calcium sensor-associated protein kinase that regulates abscisic acid and cold signal transduction in Arabidopsis. Plant Cell 15:411–423

    Article  PubMed  CAS  Google Scholar 

  • Kim KN, Lee JS, Han H, Choi SA, Go SJ, Yoon IS (2003b) Isolation and characterization of a novel rice Ca2+-regulated protein kinase gene involved in responses to diverse signals including cold, light, cytokinins, sugars and salts. Plant Mol Biol 52:1191–1202

    Article  PubMed  CAS  Google Scholar 

  • Kim KN, Cheong YH, Grant JJ, Pandey GK, Luan S (2004) CIPK3, a calcium sensor-associated protein kinase that regulates abscisic acid and cold signal transduction in Arabidopsis. Plant Cell 15:411–423

    Google Scholar 

  • Knight H (2000) Calcium signaling during abiotic stress in plants. Int Rev Cytol 195:269–324

    Article  PubMed  CAS  Google Scholar 

  • Knight H, Trewavas AJ, Knight MR (1996) Cold calcium signaling in Arabidopsis involves two cellular pools and a change in calcium signature after acclimation. Plant Cell 8:489–503

    PubMed  CAS  Google Scholar 

  • Knight H, Trewavas AJ, Knight MR (1997) Calcium signaling in Arabidopsis thaliana responding to drought and salinity. Plant J 12:1067–1078

    Article  PubMed  CAS  Google Scholar 

  • Knight H, Brandt S, Knight MR (1998) A history of stress alters drought calcium signalling pathways in Arabidopsis. Plant J 16:681–687

    Article  PubMed  CAS  Google Scholar 

  • Kolukisaoglu Ü, Weinl S, Blazevic D, Batistic O, Kudla J (2004) Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks. Plant Physiol 134:43–58

    Article  PubMed  CAS  Google Scholar 

  • Kudla J, Batisti O (2004) Integration and channeling of calcium signaling through the CBL calcium sensor/CIPK protein kinase network. Planta 219:915–924

    Article  PubMed  Google Scholar 

  • Lecourieux D, Mazars C, Pauly N, Ranjeva R, Pugin A (2002) Analysis and effects of cytosolic free calcium increases in response to elicitors in Nicotiana plumbaginifolia cells. Plant Cell 14:2627–2641

    Article  PubMed  CAS  Google Scholar 

  • Lin CC, Kao CH (2001) Abscisic acid induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings. Plant Sci 160:323–329

    Article  PubMed  CAS  Google Scholar 

  • Luan S, Kudla J, Rodriguez-Concepcion M, Yalovsky S, Gruissem W (2002) Calmodulins and calcineurin B-like proteins: calcium sensors for specific signal response coupling in plants. Plant Cell 14:S389–S400

    PubMed  CAS  Google Scholar 

  • Luan S, Lan W, Chul Lee S (2009) Potassium nutrition, sodium toxicity, and calcium signaling: connections through the CBL-CIPK network. Curr Opin Plant Biol 12:339–346

    Article  PubMed  CAS  Google Scholar 

  • Ma YY, Song WY, Liu ZH, Zhang HM, Guo XL, Shao HB, Ni FT (2009) The dynamic changing of Ca2+ cellular localization in maize leaflets under drought stress. Cell Biol/Biologie cellulaire (C.R. Biologies) 332:351–362

    CAS  Google Scholar 

  • Mahajan S, Sopory SK, Tuteja N (2006) Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum). FEBS J 273:907–925

    Article  PubMed  CAS  Google Scholar 

  • McAinsh MR, Brownlee C, Hetherington AM (1990) Abscisic acid induced elevation of guard cell cytoplasmic Ca2+ precedes stomatal closure. Nature 343:186–188

    Article  CAS  Google Scholar 

  • Pei ZM, Murata Y, Benning G, Thomine S, Klusener B, Allen GJ, Grill E, Schroeder JI (2000) Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature 406:731–734

    Article  PubMed  CAS  Google Scholar 

  • Piao HL, Xuan YH, Park SH, Je BI, Park SJ, Park SH, Kim CM, Huang J, Wang GK, Kim MJ, Kang SM, Lee IJ, Kwon TR, Kim YH, Yeo US, Yi G, Son D, Han CD (2010) OsCIPK31, a CBL-interacting protein kinase is involved in germination and seedling growth under abiotic stress conditions in rice plants. Mol Cells 30:19–27

    Article  PubMed  CAS  Google Scholar 

  • Price AH, Taylor A, Ripley SJ, Griffiths A, Trewavas AJ, Knight MR (1994) Oxidative signals in tobacco increase cytosolic calcium. Plant Cell 6:1301–1310

    PubMed  CAS  Google Scholar 

  • Reddy AS (2001) Calcium: silver bullet in signaling. Plant Sci 160:381–404

    Article  PubMed  CAS  Google Scholar 

  • Rentel MC, Knight MR (2004) Oxidative stress-induced calcium signaling in Arabidopsis. Plant Physiol 135:1471–1479

    Article  PubMed  CAS  Google Scholar 

  • Sanders D, Pelloux J, Brownlee C, Harper JF (2002) Calcium at the crossroads of signaling. Plant Cell 14:S401–S417

    PubMed  CAS  Google Scholar 

  • Shi J, Kim KN, Ritz O, Albrecht V, Gupta R, Harter K, Luan S, Kudla J (1999) Novel protein kinases associated with calcineurin B-like calcium sensors in Arabidopsis. Plant Cell 11:2393–2405

    PubMed  CAS  Google Scholar 

  • Tähtiharju S, Sangwan V, Monroy AF, Dhindsa RS, Borg M (1997) The induction of kin genes in cold-acclimating Arabidopsis thaliana. Evidence of a role for calcium. Planta 203:442–447

    Article  PubMed  Google Scholar 

  • Trewavas AJ, Malho R (1998) Ca2+ signaling in plant cells: the big network! Curr Opin Plant Biol 1:428–433

    Article  PubMed  CAS  Google Scholar 

  • Wilkinson S, Davies WJ (2002) ABA-based chemical signalling: the co-ordination of responses to stress in plants. Plant, Cell Environ 25:195–210

    Article  CAS  Google Scholar 

  • Wu SJ, Wu JY (2008) Extracellular ATP-induced NO production and its dependence on membrane Ca2+ flux in Salvia miltiorrhiza hairy roots. J Exp Bot 59:4007–4016

    Article  PubMed  CAS  Google Scholar 

  • Xiang Y, Huang YM, Xiong LZ (2007) Characterization of stress-responsive CIPK genes in rice for stress tolerance improvement 1. Plant Physiol 144:1416–1428

    Article  PubMed  CAS  Google Scholar 

  • Xiong J, Fu GF, Yang YG, Zhu C, Tao LX (2012) Tungstate: is it really a specific nitrate reductase inhibitor in plant nitric oxide research? J Exp Bot 63:33–41

    Article  PubMed  CAS  Google Scholar 

  • Xu J, Yin HX, Liu XJ, Yuan T, Mi Q, Yang LL, Xie ZX, Wang WY (2009) Nitric oxide alleviates Fe deficiency-induced stress in Solanum nigrum. Biol Plantarum 53:784–788

    Article  CAS  Google Scholar 

  • Ye NH, Zhu GH, Liu YG, Li YX, Zhang JH (2011) ABA controls H2O2 accumulation through the induction of OsCATB in rice leaves under water stress. Plant Cell Physiol 52:689–698

    Article  PubMed  CAS  Google Scholar 

  • Zhao JF, Sun ZF, Zheng J, Guo XY, Dong ZG, Huai JL, Gou MY, He JG, Jin YS, Wang JH, Wang GY (2009) Cloning and characterization of a novel CBL-interacting protein kinase from maize. Plant Mol Biol 69:661–674

    Article  PubMed  CAS  Google Scholar 

  • Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273

    Article  PubMed  CAS  Google Scholar 

  • Zou JJ, Wei FJ, Wang C, Wu JJ, Ratnasekera D, Liu WX, Wu WH (2010) Arabidopsis calcium-dependent protein kinase CPK10 functions in abscisic acid- and Ca2+-mediated stomatal regulation in response to drought stress. Plant Physiol 154:1232–1243

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (31100200) and the Natural Science Foundation of Henan Educational Committee (2011B180022).

Author information

Authors and Affiliations

  1. Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, College of Life Science, Henan Agricultural University, 450002, Zhengzhou, China

    Fuju Tai, Qi Wang, Zuli Yuan, Zhiheng Yuan, Huiyun Li & Wei Wang

Authors
  1. Fuju Tai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qi Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zuli Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhiheng Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Huiyun Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Wei Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wei Wang.

Additional information

Communicated by P. Sowinski.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tai, F., Wang, Q., Yuan, Z. et al. Characterization of five CIPK genes expressions in maize under water stress. Acta Physiol Plant 35, 1555–1564 (2013). https://doi.org/10.1007/s11738-012-1197-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-012-1197-2

Keywords

Search

Navigation