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Effect of the anthocyanic epidermal layer on Photosystem II and I energy dissipation processes in Tradescantia pallida (Rose) Hunt

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Abstract

The effect of anthocyanic cells of the epidermal layer was investigated on photosynthetic activity of the higher plant Tradescantia pallida. To determine the possible indirect role of anthocyanin in photosynthesis, analysis was done on intact leaves and leaves where anthocyanic epidermal layer was removed. Energy dissipation processes related to Photosystem II (PSII) and Photosystem I (PSI) activity was done using simultaneously Chlorophyll a (Chl a) fluorescence and P700 transmittance signals change. In anthocyanic epidermal-less leaves, PSII photochemical activity was more decreased in dependence to increasing light irradiance exposure. We found that photoinhibition of PSII decreased PSI activity by reducing the electron flow toward PSI, especially under high light intensities. Under those conditions, it resulted in the accumulation of oxidized PSI reaction centers, which was stronger in leaves where the anthocyanic epidermal layer was removed. In conclusion, our results showed that the anthocyanic epidermal layer had a photoprotective effect only on the PSII and not on the PSI of T. pallida leaves, supporting the role of anthocyanin pigments in the regulation of photosynthesis for excess absorbed light irradiance.

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Abbreviations

Chl:

Chlorophyll

ETRI:

Electron transport rate of PSI

ETRII:

Electron transport rate of PSII

LHC:

Light-harvesting complexes

PSI:

Photosystem I

PSII:

Photosystem II

UV:

Ultraviolet

ΦMII:

Maximum PSII quantum yield

\( \Upphi_{\text{M}}^{\prime } {\text{I}} \) :

Operational quantum yield of PSI

\( \Upphi_{\text{M}}^{\prime } {\text{II}} \) :

Operational quantum yield of PSII

\( \Upphi_{\text{NPQ}}^{\prime } \) :

Quantum yield for regulated non-photochemical energy dissipation of PSII

\( \Upphi_{\text{NO}}^{\prime } \) :

Quantum yield for non-regulated non-photochemical energy dissipation of PSII

\( \Upphi_{\text{NA}}^{\prime } \) :

Quantum yield for non-photochemical energy dissipation caused by acceptor side limitation of PSI

\( \Upphi_{\text{ND}}^{\prime } \) :

Quantum yield for non-photochemical energy dissipation caused by donor side limitation of PSI

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Acknowledgments

D. Dewez acknowledges financial support from Natural Sciences and Engineering Research Council (NSERC, Canada). F. Perreault was supported by a NSERC doctoral fellowship.

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We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Chemistry, University of Quebec in Montreal, C.P. 8888, Succ. Centre-Ville, Montreal, Quebec, H3C 3P8, Canada

    David Dewez & François Perreault

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  1. David Dewez
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  2. François Perreault
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Correspondence to David Dewez.

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Communicated by Z. Gombos.

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Dewez, D., Perreault, F. Effect of the anthocyanic epidermal layer on Photosystem II and I energy dissipation processes in Tradescantia pallida (Rose) Hunt. Acta Physiol Plant 35, 463–472 (2013). https://doi.org/10.1007/s11738-012-1089-5

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  • DOI: https://doi.org/10.1007/s11738-012-1089-5

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