Skip to main content
SpringerLink
Log in

Nanosecond flash studies of the absorption spectrum of the Photosystem I primary acceptor Ao

  • Regular Papers
  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

Photosystem I particles devoid of the secondary electron acceptor A1 were studied by nanosecond flash absorption. The primary radical pair (P-700+, A0 -) decays with a half-time of 35 ns. The difference spectrum was measured (400–870 nm). After subtraction of the P-700+/P-700 difference spectrum, the A0 -/A0 was obtained. It includes bleachings centered at 690 and 430 nm, and broad positive bands in the near infra-red and the blue-green. This spectrum is consistent with A0 being chlorophyll a absorbing at 690 nm.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Biggins J and Mathis M (1987) The functional role of vitamin K1 in the Photosystem I of the cyanobacterium Synechocystis 6803. Biochemistry, in press

  • Bonnerjea J and Evans MCW (1982) Identification of multiple components in the intermediary electron carrier complex of photosystem I. Febs Lett 148: 313–316

    Google Scholar 

  • Fujita I, Davis MS and Fajer J (1978) Anion Radicals of Pheophytin and Chlorophyll a: Their Role in the Primary Charge Separations of Plant Photosynthesis. J Am Chem Soc 100: 6280–6282

    Google Scholar 

  • Ikegami I and Itoh (1986) Chlorophyll organization in P-700-enriched particles isolated from spinach chloroplasts. CD and absorption spectroscopy. Biochim Biophys Acta 851: 75–85

    Google Scholar 

  • Ikegami I and Katoh S (1975) Enrichment of photosystem I reaction center chlorophyll from spinach chloroplasts. Biochim Biophys Acta 376: 588–592

    Google Scholar 

  • Ikegami I and Ke B (1984) A 160-kilodalton photosystem-I reaction-center complex. Low-temperature absorption and EPR spectroscopy of the early electron acceptors. Biochim Biophys Acta 764: 70–79

    Google Scholar 

  • Ikegami I, Sétif P and Mathis P (1987) Absorption studies of Photosystem I photochemistry in the absence of vitamin K1. Biochim Biophys Acta 894: 414–422

    Google Scholar 

  • Itoh S, Iwaki M and Ikegami I (1987) Extraction of vtiamin K1 in photosystem I particles by the treatment with diethylether and its effects on A1 EPR signal and photochemistry. Biochim Biophys Acta 893: 503–516

    Google Scholar 

  • Ke B (1973) The Primary Electron Acceptor of Photosystem I. Biochim Biophys Acta 301: 1–33.

    Google Scholar 

  • Mansfield RW and Evans MCW (1985) Optical difference spectrum of the electron acceptor Ao in Photosystem I. Febs Lett 190: 237–241

    Google Scholar 

  • Mathis P and Rutherford AW (1987) The primary reactions of photosystems I and II of algae and higher plants. In: Amesz J (ed.) Photosynthesis. pp. 63–96. Amsterdam: Elsevier.

    Google Scholar 

  • Nuijs AM, Shuvalov VA, Van Gorkom HJ, Plijter JJ and Duysens LNM (1986) Picosecond absorbance difference spectroscopy on the primary reactions and the antenna excited states in Photosystem I particles. Biochim Biophys Acta 850: 310–318

    Google Scholar 

  • Sétif P, Bottin H and Mathis P (1985) Absorption studies of primary reactions in Photosystem I; yield and rate of formation of the P700 triplet state. Biochim Biophys Acta 808: 112–122

    Google Scholar 

  • Sétif P, Ikegami I and Biggins J (1987) Light-induced charge separation in Photosystem I at low temperature is not influenced by vitamin K1. Biochim Biophys Acta 894: 146–156

    Google Scholar 

  • Shuvalov VA, Ke B and Dolan E (1979a) Kinetic and spectral properties of the intermediary electron acceptor A1 in Photosystem I. Febs Lett 100: 5–8

    Google Scholar 

  • Shuvalov VA, Klevanik AV, Sharkov AV, Kryukov PG and Ke B (1979b) Picosecond spectroscopy of Photosystem I reaction centers. Febs Lett 107: 313–316

    Google Scholar 

  • Shuvalov VA, Nuijs AM, van Gorkom HJ, Smith HWJ and Duysens LNM (1986) Picosecond absorbance changes upon selective excitation of the primary electron donor P-700 in Photosystem I. Biochim Biophys Acta 850: 319–323

    Google Scholar 

  • Swarthoff T, Gast P, Amesz J and Buisman HP (1982) Photoaccumulation of reduced primary electron acceptors of Photosystem I of photosynthesis. Febs Lett 146: 129–132

    Google Scholar 

  • Takahashi Y, Hansson O, Mathis P and Satoh K (1987) Primary radical pair in the photosystem II reaction center. Biochim Biophys Acta 893: 49–59

    Google Scholar 

  • Wasielewski MR, Fenton JM and Govindjee (1987) The rate of formation of P700+ Ao - in photosystem I particles from spinach as measured by picosecond transient absorption spectroscopy. Photosynth Res 12: 181–190

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Service de Biophysquie, Département de Biologie, CEN Saclay, 91191, Gif-sur-Yvette Cedex, France

    P. Mathis & P. Setif

  2. Faculty of Pharmaceutical Sciences, Teikyo University, 199-01, Sagamiko, Kanagawa, Japan

    I. Ikegami

Authors
  1. P. Mathis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Ikegami
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Setif
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mathis, P., Ikegami, I. & Setif, P. Nanosecond flash studies of the absorption spectrum of the Photosystem I primary acceptor Ao . Photosynth Res 16, 203–210 (1988). https://doi.org/10.1007/BF00028839

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00028839

Key words

Search

Navigation