Skip to main content
SpringerLink
Log in

Effects of exogenous hydrogen sulfide on the ascorbate and glutathione metabolism in wheat seedlings leaves under water stress

  • Short Communication
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

This study investigated the effects of exogenous hydrogen sulfide on the ascorbate and glutathione metabolism in wheat seedlings leaves under water stress. The results showed that pretreatment with sodium hydrosulfide (NaHS), hydrogen sulfide donor, increased the activities of ascorbate peroxidase, glutathione reductase, dehydroascorbate reductase and gamma-glutamylcysteine synthetase, and the contents of reduced ascorbic acid, reduced glutathione, total ascorbate and total glutathione under water stress, compared to control and water stress without NaHS. Meanwhile, pretreatment with NaHS decreased the malondialdehyde content and electrolyte leakage induced by water stress in plants, compared to control and water stress without NaHS. Our results suggested that exogenous hydrogen sulfide alleviated oxidative damage by regulating the ascorbate and glutathione metabolism in wheat seedlings under water stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Abbreviations

APX:

Ascorbate peroxidase

GR:

Glutathione reductase

MDHAR:

Monodehydroascorbate reductase

DHAR:

Dehydroascorbate reductase

GalLDH:

l-Galactono-1,4-lactone dehydrogenase

γ-ECS:

Gamma-glutamylcysteine synthetase

GSH:

Reduced glutathione

AsA:

Reduced ascorbic acid

NaHS:

Sodium hydrosulfide

H2S:

Hydrogen sulfide

References

  • Ai L, Li ZH, Xie ZX, Tian XL, Eneji AE, Duan LS (2008) Coronatine alleviates polyethylene glycol-induced water stress in two rice (Oryza sativa L.) cultivars. J Agron Crop Sci 194:360–368

    Article  CAS  Google Scholar 

  • Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399

    Article  PubMed  CAS  Google Scholar 

  • Arasimowicz M, Floryszak-Wieczorek J (2007) Nitric oxide as a bioactive signalling molecule in plant stress responses. Plant Sci 172:876–887

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Dalton DA, Russell SA, Hanus FJ, Pascoe GA, Evans HJ (1986) Enzymatic reactions of ascorbate and glutathione that prevent peroxide damage in soybean root nodules. Proc Natl Acad Sci USA 83:3811–3815

    Article  PubMed  CAS  Google Scholar 

  • Dringen R (2000) Glutathione metabolism and oxidative stress in neurodegeneration. Eur J Biochem 267:4903

    Article  PubMed  CAS  Google Scholar 

  • Grace SC, Logan BA (1996) Acclimation of foliar antioxidant systems to growth irradiance in three broad-leaved evergreen species. Plant Physiol 112:1631–1640

    PubMed  CAS  Google Scholar 

  • Griffith OW (1980) Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem 106:207–212

    Article  PubMed  CAS  Google Scholar 

  • Hodges DM, Andrews CJ, Johnson DA, Hamilton RI (1996) Antioxidant compound responses to chilling stress in differentially sensitive inbred maize lines. Plant Physiol 98:685–692

    Article  CAS  Google Scholar 

  • Hodges MD, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611

    Article  CAS  Google Scholar 

  • Hosoki R, Matsuki N, Kimura H (1997) The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide. Biochem Biophys Res Commun 237:527–531

    Article  PubMed  CAS  Google Scholar 

  • Hossain MA, Asada K (1984) Purification of dehydroascorbate reductase from spinach and its characterization as a thiol enzyme. Plant Cell Physiol 25:85–92

    CAS  Google Scholar 

  • Hu X, Wang W, Li C, Zhang J, Lin F, Zhang A, Jiang M (2008) Cross-talks between Ca2+/CaM and H2O2 in abscisic acid-induced antioxidant defense in leaves of maize plants exposed to water stress. Plant Growth Regul 55:183–198

    Article  Google Scholar 

  • Jiang MY, Zhang JH (2002) Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves. J Exp Bot 53:2401–2410

    Article  PubMed  CAS  Google Scholar 

  • Jiang M, Zhang J (2003) Cross-talk between calcium and reactive oxygen species originated from NADPH oxidase in abscisic acid-induced antioxidant defence in leaves of maize seedlings. Plant Cell Environ 26:929–939

    Article  PubMed  CAS  Google Scholar 

  • Li D, Xiao Z, Liu L, Wang J, Song G, Bi Y (2010) Effects of exogenous hydrogen sulfide (H2S) on the root tip and root border cells of Pisum sativum. Chin Bull Bot 3:354–362

    Google Scholar 

  • Li L, Van Staden J, Jäger AK (1998) Effects of plant growth regulators on the antioxidant system in seedlings of two maize cultivars subjected to water stress. Plant Growth Regul 25:81–87

    Article  CAS  Google Scholar 

  • Ma J (2007) Effects of sodium hydrosulfide on the antioxidative systems and cyanide-resistant respiration in rice suspension cell. Dissertation, Lanzhou University

  • Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410

    Article  PubMed  CAS  Google Scholar 

  • Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498

    Article  PubMed  CAS  Google Scholar 

  • Miyake C, Asada K (1992) Thylakoid-bound ascorbate peroxidase in spinach chloroplasts and photoreduction of its primary oxidation product monodehydroascorbate radicals in thylakoids. Plant Cell Physiol 33:541–553

    CAS  Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880

    CAS  Google Scholar 

  • Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol 49:249–279

    Article  CAS  Google Scholar 

  • Papadakis AK, Roubelakis-Angelakis KA (2005) Polyamines inhibit NADPH oxidase-mediated superoxides generation and putrescine prevents programmed cell death syndrome induced by the polyamine oxidase generated hydrogen peroxide. Planta 220:826–837

    Article  PubMed  CAS  Google Scholar 

  • Rao MV, Paliyath G, Ormrod DP (1996) Ultraviolet-B- and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiol 110:125–136

    Article  PubMed  CAS  Google Scholar 

  • Rüegsegger A, Brunold C (1992) Effect of cadmium on γ-glutamylcysteine synthesis in maize seedlings. Plant Physiol 99:428–433

    Article  PubMed  Google Scholar 

  • Schafer FQ, Buettner GR (2001) Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. Free Rad Biol Med 30:1191–1212

    Article  PubMed  CAS  Google Scholar 

  • Shanker AK, Djanaguiraman M, Sudhagar R, Chandrashekar CN, Pathmanabhan G (2004) Differential antioxidative response of ascorbate glutathione pathway enzymes and metabolites to chromium speciation stress in green gram (Vigna radiata (L.) R.Wilczek. cv CO 4) roots. Plant Sci 166:1035–1043

    Article  CAS  Google Scholar 

  • Smirnoff N (1996) The function and metabolism of ascorbic acid in plants. Ann Bot 78:661–669

    Article  CAS  Google Scholar 

  • Tabata K, Oba K, Suzuki K, Esaka M (2001) Generation and properties of ascorbic acid-deficient transgenic tobacco cells expressing antisense RNA of l-galactono-1, 4-lactone dehydrogenase. Plant J 27:139–148

    Article  PubMed  CAS  Google Scholar 

  • Wendehenne D, Dumer J, Klessing DF (2004) Nitric oxide: a new player in plant signaling and defense responses. Curr Opin Plant Biol 7:449–455

    Article  PubMed  CAS  Google Scholar 

  • Wheeler GL, Jones MA, Smirnoff N (1998) The biosynthetic pathway of vitamin C in higher plants. Nature 393:365–369

    Article  PubMed  CAS  Google Scholar 

  • Zhang H, Hu LY, Hu KD, He YD, Wang SH, Luo JP (2008) Hydrogen sulfide promotes wheat seed germination and alleviates the oxidative damage against copper stress. J Integr Plant Biol 50:1518–1529

    Article  PubMed  CAS  Google Scholar 

  • Zhang H, Tang J, Liu XP, Wang Y, Yu W, Peng WY, Fang F, Ma DF, Wei ZJ, Hu LY (2009a) Hydrogen sulfide promotes root organogenesis in Ipomoea batatas, Salix matsudana and Glycine max. J Integr Plant Biol 51:1086–1094

    Article  PubMed  CAS  Google Scholar 

  • Zhang H, Ye YK, Wang SH, Luo JP, Tang J, Ma DF (2009b) Hydrogen sulfide counteracts chlorophyll loss in sweetpotato seedling leaves and alleviates oxidative damage against osmotic stress. Plant Growth Regul 58:243–250

    Article  CAS  Google Scholar 

  • Zhang H, Wang MJ, Hu LY, Wang SH, Hu KD, Bao LJ, Luo JP (2010a) Hydrogen sulfide promotes wheat seed germination under osmotic stress. Russ J Plant Physiol 57:532–539

    Article  CAS  Google Scholar 

  • Zhang H, Hu LY, Li P, Hu KD, Jiang CX, Luo JP (2010b) Hydrogen sulfide alleviated chromium toxicity in wheat. Biol Plant 54:743–747

    Article  CAS  Google Scholar 

  • Zhang H, Jiao H, Jiang CX, Wang SH, Wei ZJ, Luo JP, Jones RL (2010c) Hydrogen sulfide protects soybean seedlings against drought-induced oxidative stress. Acta Physiol Plant 32:849–857

    Article  CAS  Google Scholar 

  • Zhang H, Tan ZQ, Hu LY, Wang SH, Luo JP, Jones RL (2010d) Hydrogen sulfide alleviates aluminum toxicity in germinating wheat seedlings. J Integr Plant Biol 52:556–567

    Article  PubMed  CAS  Google Scholar 

  • Zhao LQ, Zhang F, Guo JK, Yang YL, Li BB, Zhang LX (2004) Nitric oxide functions as a signal in salt resistance in the calluses from two ecotypes of reed. Plant Physiol 134:849–857

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the Important Scientific Research Project of Henan Institute of Science and Technology (040124).

Author information

Authors and Affiliations

  1. Henan Institute of Science and Technology, Xinxiang, Henan, 453003, China

    Chang-juan Shan, Sheng-li Zhang, Dong-fang Li, Yuan-zeng Zhao, Xue-liang Tian, Xin-liang Zhao, Ying-xia Wu, Xiu-ying Wei & Run-qiang Liu

Authors
  1. Chang-juan Shan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sheng-li Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dong-fang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuan-zeng Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xue-liang Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xin-liang Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ying-xia Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xiu-ying Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Run-qiang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chang-juan Shan.

Additional information

Communicated by L. A. Kleczkowski.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shan, Cj., Zhang, Sl., Li, Df. et al. Effects of exogenous hydrogen sulfide on the ascorbate and glutathione metabolism in wheat seedlings leaves under water stress. Acta Physiol Plant 33, 2533–2540 (2011). https://doi.org/10.1007/s11738-011-0746-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-011-0746-4

Keywords

Search

Navigation