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Protein differential expression in the latex from Hevea brasiliensis between self-rooting juvenile clones and donor clones

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Abstract

To better understand molecular mechanism underlying the difference between self-rooting juvenile clones and donor clones, a proteomic approach was used to profile protein changes in the latex between self-rooting juvenile clones and donor clones. Total soluble proteins were extracted from latex in self-rooting juvenile clones and donor clones. Two-dimensional gel electrophoresis (2-DE) was used to identify proteins that were differentially expressed in self-rooting juvenile clones and donor clones and image analysis was used to determine which proteins were up- or down-regulated. Twenty-four differentially expressed protein spots were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Among the 24 proteins identified, 13 proteins were up-regulated, 11 proteins were decreased in self-rooting juvenile clones. These proteins were classified as carbohydrate and energy metabolism, secondary metabolism, signal translocation, transcriptional regulation-related, protein synthesis and degradation, transport, nucleoside acid process, lipid metabolism. Perhaps, the present study contributes towards an understanding of the molecular mechanism underlying the difference between self-rooting juvenile clones and donor clones.

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Abbreviations

CBB:

Coomassie brilliant blue

DCs:

Donor clones

GAPDH:

Glyceraldehyde 3-phosphate dehydrogenase

pI:

Isoelectric point

JCs:

Juvenile clones

MALDI-TOF MS:

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry

1-DE:

One-dimensional gel electrophoresis

PAGE:

Polyacrylamide gel electrophoresis

REF:

Rubber elongation factor

SOD:

Superoxide dismutase

2-DE:

Two-dimensional gel electrophoresis

References

  • Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 53:1331–1341

    Article  PubMed  CAS  Google Scholar 

  • Carron MP, Enjalric F, Lardet L, Deschamps A (1989) Rubber (Hevea brasiliensis Muell. Arg.). In: Bajaj (ed) Biotechnology in agriculture and forestry, vol 5. Springer, Germany, pp 222–245

  • Carron MP, Granent F, Keli J (2007) Budding from rejuvenated clones: a good compromise between micropropagation and conventional budding. International rubber Conference, Siem Reap, Cambodia, pp 367–373

  • Chandrashekar TR, Mydin KK, Alice J, Varghese YA, Saraswathyamma CK (1997) Intraclonal variability for yield in rubber (Hevea brasiliensis). Ind J Nat Rubb Res 10:43–47

    Google Scholar 

  • Chen XT, Wang ZY, Wu HD, Xie YP, Zheng WR (1998) In vitro micropropagation of juvenile self-root clones of Hevea brasiliensis. Acta Agron Sin 24:225–230

    Google Scholar 

  • Chen XT, Wang ZY, Wu HD, Zhang XJ (2001) Selection of optimum planting material of Hevea brasiliensis: self-rooting juvenile clone. In: Sainte-Beuve J (ed) Annual IRRDB meeting CIRAD, Montpellier, France

  • Chen XT, Wang ZY, Wu HD, Zhang XJ (2002) A new planting material of Hevea brasiliensis—self-rooting juvenile-type clone. Chin J Trop Crops 23:1923

    Google Scholar 

  • Chow KS, Wan KL, Mat IMN, Bahari A, Tan SH, Harikrishna K, Yeang HY (2007) Insights into rubber biosynthesis from transcriptome analysis of Hevea brasiliensis latex. J Exp Bot 58:2429–2440

    Article  PubMed  CAS  Google Scholar 

  • Chrestin H, Gidrol X, Kush A (1997) Towards a latex molecular diagnostic of yield potential and the genetic engineering of the rubber tree. Euphytica 96:77–82

    Article  CAS  Google Scholar 

  • Clément-Demange A, Priyadarshan PM, Hoa TTT, Venkatachalam P (2007) Hevea rubber breeding and genetics. Plant Breed Rev 29:177–281

    Article  Google Scholar 

  • d’Auzac J (1989) Factors involved in the stopping of latex flow after tapping. In: d’Auzac J, Chrestin (eds) Physiology of the rubber tree latex. CRC Press, Inc., Boca Raton, pp 257–280

  • Dennis MS, Light DR (1989) Rubber elongation factor from Hevea brasiliensis: identification, characterization and role in rubber biosynthesis. J Biol Chem 264:18608–18617

    PubMed  CAS  Google Scholar 

  • Hao BZ, Wu JL (1996) The high-yield characteristics of rubber tree juvenile clone. Trop Agric Sci 2:1–8

    Google Scholar 

  • Holtgrefe S, Gohlke J, Starmann J, Druce S, Klocke S, Altmann B, Wojtera J, Lindermayr C, Scheibe R (2008) Regulation of plant cytosolic glyceraldehyde 3-phosphate dehydrogenase isoforms by thiol modifications. Physiol Plant 133:211–228

    Article  PubMed  CAS  Google Scholar 

  • Hua YW, Huang TD, Huang HS (2010) Micropropagation of self-rooting juvenile clones by secondary somatic embryogenesis in Hevea brasiliensis. Plant Breed 129:202–207

    Article  CAS  Google Scholar 

  • Kostyal DA, Hickey VL, Noti JD, Sussman GL, Beezhold DH (1998) Cloning and characterization of a latex allergen (Hev b 7): homology to patatin, a plant PLA2. Clin Exp Immunol 112:355–362

    Google Scholar 

  • Kush A (1994) Isoprenoid biosynthesis: the Hevea factory. Plant Physiol Biochem 32:761–767

    CAS  Google Scholar 

  • Li DJ, Yan J, Deng Z, Chen CL, He P, Wu M, Chen SC (2009) Evaluation of three methods for protein extraction suitable for 2-DE of Hevea brasiliensis C-serum. Chin Agric Sci Bull 25:273–279

    Google Scholar 

  • Li HL, Lu HZ, Guo D, Tian WM, Peng SQ (2011) Molecular characterization of a thioredoxin h gene (HbTRX1) from Hevea brasiliensis showing differential expression in latex between self-rooting juvenile clones and donor clones. Mol Biol Rep 38:1989–1994

    Google Scholar 

  • Liang XL, Li HL, Peng SQ (2009) Cloning and expression of HbTCTP from Hevea brasiliensis. Mol Plant Breed 7:194–198

    Google Scholar 

  • Liu SQ, Yuan XH, Huang X, Xu LY (1985) Comparative studies on yield and properties of juvenile type- and its mature type clones. Trop Crop Res 3:1–5

    Google Scholar 

  • Lu HZ, Li HL, Guo D, Peng SQ (2010) Cloning and expression of a HbHDT1 gene from Hevea brasiliensis. Chinese J Trop Crops 31:59–64

    Google Scholar 

  • Priya P, Venkatachalam P, Thulaseedharan A (2007) Differential expression pattern of rubber elongation factor (REF) mRNA transcripts from high and low yielding clones of rubber tree (Hevea brasiliensis Muell. Arg.). Plant Cell Rep 26:1833–1838

    Article  PubMed  CAS  Google Scholar 

  • Venkatachalam P, Thulaseedharan A, Raghothama K (2007) Identification of expression profiles of tapping panel dryness (TPD) associated genes from the latex of rubber tree (Hevea brasiliensis Muell. Arg.). Planta 226:499–515

    Article  PubMed  CAS  Google Scholar 

  • Wang Z, Zeng X, Chen C, Wu H, Li Q, Fan G, Lu W (1980) Induction of rubber plantlets from anther of Hevea brasiliensis in vitro. Chinese J Trop Crops 1:25–26

    Google Scholar 

  • Wang X, Shi M, Lu X, Ma R, Wu C, Guo A, Peng M, Tian W (2010) A method for protein extraction from different subcellular fractions of laticifer latex in Hevea brasiliensis compatible with 2-DE and MS. Proteome Sci 8:35

    Article  PubMed  Google Scholar 

  • Yang SQ, Mo YY (1994) Some physiological properties of latex from somatic plants of Hevea brasiliensis. Chinese J Trop Crops 15:13–20

    Google Scholar 

  • Yuan XH, Yang SQ, Xu LY, Wu JL, Hao BZ (1998) Characteristics related to higher rubber yield of Hevea brasiliensis juvenile-type clone Gl1. J Rubber Res 1:125–132

    CAS  Google Scholar 

  • Zaffagnini M, Michelet L, Marchand C, Sparla F, Decottignies P, Maréchal PL, Miginiac-Maslow M, Noctor G, Trost P, Lemaire SD (2007) The thioredoxin-independent isoform of chloroplastic glyceraldehyde-3-phosphate dehydrogenase is selectively regulated by glutathionylation. FEBS J 274:212–226

    Google Scholar 

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (nos. 30960307) and a Chinese National Nonprofit Institute Research Grant of CATAS-ITBB (ITBB110205). We deeply thank Professor Xiong-Ting Chen (Institute of Tropical Biosciences and Biotechnology, CATAS) for his collaboration in treatment of plant materials. We appreciate technical assistance from Dr Xu-chu Wang of Institute of Tropical Biosciences and Biotechnology at CATAS.

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Authors and Affiliations

  1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Hui-Liang Li, Dong Guo, Fang-Ying Lan & Shi-Qing Peng

  2. Key Laboratory of Rubber Biology, Ministry of Agriculture, Institute of Rubber, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, China

    Wei-Min Tian

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  1. Hui-Liang Li
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  2. Dong Guo
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  3. Fang-Ying Lan
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  4. Wei-Min Tian
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  5. Shi-Qing Peng
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Corresponding author

Correspondence to Shi-Qing Peng.

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Communicated by S. Abe.

H.-L. Li and D. Guo contributed equally to this work.

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Li, HL., Guo, D., Lan, FY. et al. Protein differential expression in the latex from Hevea brasiliensis between self-rooting juvenile clones and donor clones. Acta Physiol Plant 33, 1853–1859 (2011). https://doi.org/10.1007/s11738-011-0727-7

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  • DOI: https://doi.org/10.1007/s11738-011-0727-7

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