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Isolation of a cinnamoyl CoA reductase gene involved in formation of stone cells in pear (Pyrus pyrifolia)

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Abstract

Stone cell which appears in most pear cultivars contributes badly to pear internal quality. Lignin is the important component of stone cells and its first biosynthetic but specific step is catalyzed by cinnamoyl CoA reductase (CCR). In the present study, a cDNA encoding CCR, named as PpCCR (GenBank accession no. GU138672), was isolated from pear (Pyrus pyrifolia) fruit. Its full length is 1,247 bp with a putative maximal open reading frame of 1,017 bp, encoding a protein of 339 amino acids. Sequence alignment revealed that the deduced protein contained a typical CCR catalytic site and NADP(H) binding site. Phylogenetic analysis showed that PpCCR belonged to dicot CCR class II in which most CCRs participated in the regulation of lignin biosynthesis. PpCCR transcript could be detected in leaves, flowers and fruits. In addition, PpCCR expression profiles during fruit development were compared between two cultivars with significant difference in stone cell content in the flesh. Although PpCCR expressed in a similar pattern between the two cultivars and their transcript level consisted strictly with the change of lignin content, its transcript level was always higher in the high-lignin content cultivar than in the low-lignin content cultivar during fruit development. Therefore, it could be suggested that PpCCR is involved in the regulation of lignin biosynthesis and plays an important role in stone cell formation in pear flesh.

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References

  • Boerjan W, Ralph J, Baucher M (2003) Lignin biosynthesis. Annu Rev Plant Biol 54:519–546

    Article  CAS  PubMed  Google Scholar 

  • Bruce RJ, West CA (1989) Elicitation of lignin biosynthesis and isoperoxidase activity by pectic fragments in suspension cultures of castor bean. Plant Physiol 91:889–897

    Article  CAS  PubMed  Google Scholar 

  • Cai C, Xu CJ, Li X, Ferguson I, Chen KS (2006) Accumulation of lignin in relation to change in activities of lignification enzymes in loquat fruit flesh after harvest. Postharvest Biol Technol 40:163–169

    Article  CAS  Google Scholar 

  • Capeleti I, Bonini EA, Ferrarese MDL, Teixeira ACN, Krzyzanowski FC, Ferrarese-Filho O (2005) Lignin content and peroxidase activity in soybean seed coat susceptible and resistant to mechanical damage. Acta Physiol Plant 27:103–108

    Article  CAS  Google Scholar 

  • Chabannes M, Barakate A, Lapierre C, Marita JM, Ralph J, Pean M, Danoun S, Halpin C, Grima-Pettenati J, Boudet AM (2001) Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants. Plant J 28:257–270

    Article  CAS  PubMed  Google Scholar 

  • Choi JH, Choi JJ, Hong KH, Kim WS, Lee SH (2007) Cultivar differences of stone cells in pear flesh and their effects on fruit quality. Hortic Environ Biotechnol 48:27–31

    Google Scholar 

  • Crist JW, Batjer LP (1931) The stone cells of pear fruits, especially the kieffer pear. Mich Agric Exp Station Tech Bull 113:1–55

    Google Scholar 

  • Goujon T, Ferret V, Mila I, Pollet B, Ruel K, Burlat V, Joseleau JP, Barriere Y, Lapierre C, Jouanin L (2003) Down-regulation of the AtCCR1 gene in Arabidopsis thaliana: effects on phenotype, lignins and cell wall degradability. Planta 217:218–228

    CAS  PubMed  Google Scholar 

  • Hu CG, Honda C, Kita M, Zhang ZL, Tsuda T, Takaya M (2002) A simple protocol for RNA isolation from fruit trees containing high levels of polysaccharides and polyphenol compounds. Plant Mol Biol Rep 20:69a–69g

    Article  Google Scholar 

  • Kaczkowski J (2003) Structure, function and metabolism of plant cell wall. Acta Physiol Plant 25:287–305

    Article  CAS  Google Scholar 

  • Kozak M (1984) Compilation and analysis of sequences upstream from the translation start site in eukaryotic messenger-RNAs. Nucleic Acids Res 12:857–872

    Article  CAS  PubMed  Google Scholar 

  • Kuc J (1997) Molecular aspects of plant responses to pathogens. Acta Physiol Plant 19:551–559

    Article  CAS  Google Scholar 

  • Lacombe E, Hawkins S, VanDoorsselaere J, Piquemal J, Goffner D, Poeydomenge O, Boudet AM, GrimaPettenati J (1997) Cinnamoyl CoA reductase, the first committed enzyme of the lignin branch biosynthetic pathway: cloning, expression and phylogenetic relationships. Plant J 11:429–441

    Article  CAS  PubMed  Google Scholar 

  • Larsen K (2004a) Molecular cloning and characterization of cDNAs encoding cinnamoyl CoA reductase (CCR) from barley (Hordeum vulgare) and potato (Solanum tuberosum). J Plant Physiol 161:105–112

    Article  CAS  PubMed  Google Scholar 

  • Larsen K (2004b) Cloning and characterization of a ryegrass (Lolium perenne) gene encoding cinnamoyl-CoA reductase (CCR). Plant Sci 166:569–581

    Article  CAS  Google Scholar 

  • Lauvergeat V, Lacomme C, Lacombe E, Lasserre E, Roby D, Grima-Pettenati J (2001) Two cinnamoyl-CoA reductase (CCR) genes from Arabidopsis thaliana are differentially expressed during development and in response to infection with pathogenic bacteria. Phytochemistry 57:1187–1195

    Article  CAS  PubMed  Google Scholar 

  • Lee SH, Choi JH, Kim WS, Han TH, Park YS, Gemma H (2006) Effect of soil water stress on the development of stone cells in pear (Pyrus pyrifolia cv. ‘Niitaka’) flesh. Sci Hortic 110:247–253

    Article  Google Scholar 

  • Leple JC, Dauwe R, Morreel K, Storme V, Lapierre C, Pollet B, Naumann A, Kang KY, Kim H, Ruel K, Lefebvre A, Joseleau JP, Grima-Pettenati J, De Rycke R, Andersson-Gunneras S, Erban A, Fehrle I, Petit-Conil M, Kopka J, Polle A, Messens E, Sundberg B, Mansfield SD, Ralph J, Pilate G, Boerjan W (2007) Downregulation of cinnamoyl-coenzyme A reductase in poplar: multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure. Plant Cell 19:3669–3691

    Article  CAS  PubMed  Google Scholar 

  • Li YH, Kajita S, Kawai S, Katayama Y, Morohoshi N (2003) Down-regulation of an anionic peroxidase in transgenic aspen and its effect on lignin characteristics. J Plant Res 116:175–182

    Article  CAS  PubMed  Google Scholar 

  • Liu L, Li J, Qin WM (2005) Stone cell development and its effects on the sarcocarp of Kuerle fragrant pear. Xibei Zhiwu Xuebao 25:1965–1968

    Google Scholar 

  • Lutcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA (1987) Selection of AUG initiation codons differs in plants and animals. EMBO J 6:43–48

    CAS  PubMed  Google Scholar 

  • Ma QH (2007) Characterization of a cinnamoyl-CoA reductase that is associated with stem development in wheat. J Exp Bot 58:2011–2021

    Article  CAS  PubMed  Google Scholar 

  • McInnes R, Lidgett A, Lynch D, Huxley H, Jones E, Mahoney N, Spangenberg G (2002) Isolation and characterization of a cinnamoyl-CoA reductase gene from perennial ryegrass (Lolium perenne). J Plant Physiol 159:415–422

    Article  CAS  Google Scholar 

  • Pichon M, Courbou I, Beckert M, Boudet AM, Grima-Pettenati J (1998) Cloning and characterization of two maize cDNAs encoding Cinnamoyl-CoA Reductase (CCR) and differential expression of the corresponding genes. Plant Mol Biol 38:671–676

    Article  CAS  PubMed  Google Scholar 

  • Piquemal J, Lapierre C, Myton K, O’Connell A, Schuch W, Grima-Pettenati J, Boudet AM (1999) Down-regulation of Cinnamoyl-CoA Reductase induces significant changes of lignin profiles in transgenic tobacco plants. Plant J 13:71–78

    Article  Google Scholar 

  • Qiao YJ, Zhang SL, Tao ST, Zhang ZM, Liu ZL (2005) Advances in research on developing mechanism of stone cells in pear fruit. J Fruit Sci 22:367–371 (in Chinese with English abstract)

    Google Scholar 

  • Ranadive AS, Haard NF (1973) Chemical nature of stone cells from pear fruit. J Food Sci 38:331–333

    Article  CAS  Google Scholar 

  • Selman-Housein G, Lopezm MA, Hernandez D, Civardi L, Miranda F, Rigau J, Puigdomenech P (1999) Molecular cloning of cDNAs coding for three sugarcane enzymes involved in lignification. Plant Sci 143:163–171

    Article  CAS  Google Scholar 

  • Solecka D (1997) Role of phenylpropanoid compounds in plant responses to different stress factors. Acta Physiol Plant 19:257–268

    Article  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  • Tao ST, Khanizadeh S, Zhang H, Zhang SL (2009) Anatomy, ultrastructure and lignin distribution of stone cells in two Pyrus species. Plant Sci 176:413–419

    Article  CAS  Google Scholar 

  • Wadenback J, von Arnold S, Egertsdotter U, Walter MH, Grima-Pettenati J, Goffner D, Gellerstedt G, Gullion T, Clapham D (2008) Lignin biosynthesis in transgenic Norway spruce plants harboring an antisense construct for cinnamoyl CoA reductase (CCR). Transgenic Res 17:379–392

    Article  PubMed  Google Scholar 

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Acknowledgments

We are grateful to National Sand Pear Germplasm Repository, Wuhan, China, for kindly providing the plant materials. This work was supported by National Natural Science Foundation of China (No. 30921002).

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology (Huazhong Agricultural University), Ministry of Education, Wuhan, 430070, People’s Republic of China

    Xiao-Peng Lu, Yong-Zhong Liu, Ji-Cui An & Shu-Ang Peng

  2. Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, 430209, People’s Republic of China

    Hong-Ju Hu

  3. College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Xiao-Peng Lu, Yong-Zhong Liu, Ji-Cui An & Shu-Ang Peng

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  1. Xiao-Peng Lu
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  2. Yong-Zhong Liu
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  3. Ji-Cui An
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  4. Hong-Ju Hu
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Correspondence to Shu-Ang Peng.

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Communicated by H. Janska.

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Lu, XP., Liu, YZ., An, JC. et al. Isolation of a cinnamoyl CoA reductase gene involved in formation of stone cells in pear (Pyrus pyrifolia). Acta Physiol Plant 33, 585–591 (2011). https://doi.org/10.1007/s11738-010-0583-x

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  • DOI: https://doi.org/10.1007/s11738-010-0583-x

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