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The effect of total inorganic nitrogen and the balance between its ionic forms on adventitious bud formation and callus growth of ‘Węgierka Zwykła’ plum (Prunus domestica L.)

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Abstract

The effect of different ammonium NH +4 and nitrate NO −3 ratios (4:1, 2:1, 1:1, 1:2, 1:4, 1:6) on organogenesis of ‘Węgierka Zwykła’ leaf explants cultivated on media with nitrogen levels equalling full- or half-MS was investigated. On media with total nitrogen equal to ½ MS, explant regeneration increased significantly and was highest on media with 1:2 or 1:4 NH +4 :NO −3 ratio. An excess of ammonium versus nitrate ions had a negative effect on both regeneration and biomass. Addition of potassium to the medium increased the fresh weight of explants and the number of adventitious buds.

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Authors and Affiliations

  1. Department of Botany, Agricultural University of Kraków, 29 Listopada 54, 31-425, Kraków, Poland

    Barbara Nowak & Kazimierz Miczyński

  2. Department of Mechanical Engineering and Agrophysics, Agricultural University of Kraków, Balicka 104, 30-149, Kraków, Poland

    Ludwik Hudy

Authors
  1. Barbara Nowak
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  2. Kazimierz Miczyński
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  3. Ludwik Hudy
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Correspondence to Barbara Nowak.

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Communicated by M. Saniewski.

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Nowak, B., Miczyński, K. & Hudy, L. The effect of total inorganic nitrogen and the balance between its ionic forms on adventitious bud formation and callus growth of ‘Węgierka Zwykła’ plum (Prunus domestica L.). Acta Physiol Plant 29, 479–484 (2007). https://doi.org/10.1007/s11738-007-0058-x

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  • DOI: https://doi.org/10.1007/s11738-007-0058-x

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