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Variation in the enzyme activity and gene expression of myo-inositol-3-phosphate synthase and phytate accumulation during seed development in common bean (Phaseolus vulgaris L.)

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Abstract

The most abundant form in which phosphorus occurs in seeds is phytate (myo-inositol hexakisphosphate), mostly known as an antinutrient for animals, given its ability to complex proteins and minerals, despite its antioxidant and anticarcinogenic properties. However, phytate synthesis is still poorly understood, both in terms of its regulation and metabolic route, and relatively few works have addressed the control mechanism of phytate accumulation during seed development. Aiming at understanding the control mechanism of phytate synthesis, we examined myo-inositol-3-phosphate synthase (MIPS) (EC 5.5.1.4) activity and gene expression during seed development of common bean. Phytate concentration was low at the initial stage of seed development, coinciding with a period of the most intense seed metabolism, but followed by a period of high enzymatic activity and gene expression of MIPS when a decrease in its specific activity and transcription was detected throughout seed development until 20 days after flowering; however, the specific activity of MIPS dropped more expressively than the gene expression, matching with higher phytate concentration. Hence, we show that there is evidence of one control point regulating phytate synthesis with MIPS enzyme.

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Abbreviations

DAF:

Days after flowering

IP:

Inositol phosphates

Ins(3)P, MIPS, EC 5.5.1.4:

Myo-inositol-3-phosphate synthase

cDNA:

Deoxyribonucleic acid stand

PCR:

Polymerase chain reaction

RT:

Reverse transcriptase

P:

Phosphorus

MI-3P:

Myo-inositol 3-P

MIK:

Myo-inositol kinase

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Acknowledgments

This work was supported by The State of Sao Paulo Research Foundation (FAPESP) and The National Council for Scientific and Technological Development (CNPq), Brazil.

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Authors and Affiliations

  1. Centro Agroveterinário, Universidade do Estado de Santa Catarina, Av. Luiz de Camões, 2090, Lages, SC, CEP 88520-000, Brazil

    Cileide Maria Medeiros Coelho

  2. Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brazil

    Vagner Augusto Benedito, Antonio Figueira & Victor Alexandre Vitorello

  3. Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, Brazil

    Ricardo Antunes Azevedo

Authors
  1. Cileide Maria Medeiros Coelho
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  2. Vagner Augusto Benedito
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  3. Antonio Figueira
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  4. Victor Alexandre Vitorello
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  5. Ricardo Antunes Azevedo
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Corresponding author

Correspondence to Cileide Maria Medeiros Coelho.

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Communicated by G. Klobus.

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Coelho, C.M.M., Benedito, V.A., Figueira, A. et al. Variation in the enzyme activity and gene expression of myo-inositol-3-phosphate synthase and phytate accumulation during seed development in common bean (Phaseolus vulgaris L.) . Acta Physiol Plant 29, 265–271 (2007). https://doi.org/10.1007/s11738-007-0033-6

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  • DOI: https://doi.org/10.1007/s11738-007-0033-6

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