Skip to main content
SpringerLink
Log in

Pro-embryos induction from Olea europaea L. isolated microspore culture

  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Studies were undertaken with one olive (Olea europaea L.) cultivar to identify buds with microspores competent to embryogenesis in vitro. Isolated microspore cultures were performed for the induction of gametic embryogenesis. Different pollen development stages and stress conditions (heat or cold shock) were evaluated. The correlation of inflorescence, anther morphology and the suitable stage of microspore development were analysed. The morphology of responsive buds was identified which corresponded with microspores from the late uni-nucleate to early bi-nucleate pollen stages. Symmetrical divisions of microspores as well as resulting multinucleate structures and pro-embryos were observed. In this paper, a new method of isolated microspore culture that leads to cell division and pro-embryos in olive, is reported.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Assani A., Bakry F., Kerbellec., Haícour R., Wenzel G., Foroughi-Wehr B. 2003. Production of haploids from anther culture of banana [Musa balbisiana (BB)]. Plant Cell Rep. 21: 511–516.

    PubMed  CAS  Google Scholar 

  • Bajaj Y.P.S., Dhanju, M.S. 1983. Pollen embryogenesis in three ornamental trees Cassia fistula, Jacaranda acutifolia and Poinciana regia. J. Tree Sci. 2: 16–19.

    Google Scholar 

  • Baldursson S., Nørgaard J.V., Krogstrup P. Andersen S.B. 1993. Microspore embryogenesis in anther culture of three species of Populus, and regeneration of dihaploid plants of Populus trichocarpa. Can. J. For. Res. 23: 1812–1825.

    Google Scholar 

  • Barro F., Martin A., 1999. Response of different genotypes of Brassica carinata to microspore culture. Plant Breeding 118: 79–81

    Article  Google Scholar 

  • Bayliss K.L., Wroth J.M., Cowling W.A, 2004. Pro-embryos of Lupinus spp. produced from isolated microspore culture. Austr. J. Agricult. Res. 55: 589–593.

    Article  Google Scholar 

  • Bueno M.A., Gómez A., Boscaiu M., Manzanera J.A., Vicente O. 1997. Stress-induced formation of haploid plants through anther culture in cork-oak (Quercus suber L.). Physiol. Plant. 99: 335–341.

    Article  CAS  Google Scholar 

  • Chen Z, 1990. Rubber (Hevea brasiliensis (Muell.) Arg.): in vitro production of haploids. In: Biotechnology in Agriculture and Forestry, ed. by Y.P.S. Bajaj. Haploids in crop improvement 1. Vol. 12, Springer, Berlin: 215–236

    Google Scholar 

  • Deutsch F., Kumlehn J., Ziegenhagen B., Fladung M. 2004. Stable haploid poplar callus lines from immature pollen culture. Physiol. Plant. 120: 613–622.

    Article  PubMed  CAS  Google Scholar 

  • Guo Y.D., Pulli S. 2000. An efficient androgenic embryogenesis and plant regeneration method through isolated microspore culture in timothy (Phleum pratense L.). Plant Cell Rep. 19: 761–767.

    Article  CAS  Google Scholar 

  • Herrera J.C., Moreno L.G., Acu a J.R., De la Pe a M., Osorio D. 2002. Colchicine induced microspore embryogenesis in coffee. Plant Cell Tiss. Org. Cult. 71: 89–92.

    Article  CAS  Google Scholar 

  • Höfer M. 2004. In vitro androgenesis in apple — improvement of the induction phase. Plant Cell Rep. 22: 365–370.

    Article  PubMed  CAS  Google Scholar 

  • Höfer M., Touraev A., Heberle-Bors E. 1999. Induction of embryogenesis from isolated apple microspores. Plant Cell Rep. 18: 1012–1017.

    Article  Google Scholar 

  • Hu T., Kasha KJ. 1999. A cytological study of pretreatments used to improve isolated microspore cultures of wheat (Triticum aestivum L.) cv. Chris. Genome 42: 432–441.

    Article  Google Scholar 

  • Indrianto A., Barinova I., Touraev A., Heberle-Bors E. 2001. Tracking individual wheat microspores in vitro: identification of embryogenic microspores and body axis formation in the embryo. Planta 212: 163–174.

    Article  PubMed  CAS  Google Scholar 

  • Jähne A., Lörz H. 1995. Cereals microspore culture. Plant Sci. 109: 1–12.

    Article  Google Scholar 

  • Jörgensen J. 1988. Embryogenesis in Quercus petraea and Fagus sylvatica. J. Plant Physiol. 132: 638–640

    Google Scholar 

  • Kyo M., and Harada H. 1986. Control of the developmental pathway of tobacco pollen in vitro. Planta 168: 427–432.

    Article  CAS  Google Scholar 

  • Lichter R. 1982. Induction of haploids plants from isolated pollen of Brassica napus. Z Pflanzenphysiol. 105:427–434.

    Google Scholar 

  • Radojevic L., Jordgevic N., Tucic B. 1989. In vitro pollen embryos and plantlets in Aesculus carnea Hayne through anther culture. Plant Cell. Tiss. Org. Cult. 17: 21–26.

    Google Scholar 

  • Thanh-Tuyen N.T., De Guzman E.V. 1983. Formation of pollen embryos in cultured anthers of coconut (Cocos nucifera L.). Plant Sci. Lett 29: 81–88.

    Article  Google Scholar 

  • Touraev A., Vicente O., Heberle-Bors E. 1997. Initiation of microspore embryogenesis by stress. Trends Plant Sci. 2:297–302.

    Article  Google Scholar 

  • Zaki M.A.M., Dickinson H.G. 1991. Microspore-derived embryos in Brassica: the significance of division symmetry in pollen mitosis I to embryogenic development. Sex. Plant Reprod. 4: 48–55.

    Article  Google Scholar 

  • Zheng Y.M. 2003. Microspore culture in wheat (Triticum aestivum)-doubled haploid production via induced embryogenesis. Plant Cell Tiss. Org. Cult. 73: 213–230.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Ctra. de la Coru a km 7,5, 28040, Madrid, Spain

    María Angeles Bueno & Beatriz Pintos

  2. Bundessanstat. für Züchtungsforschung an Kulturpfanzen, Institut für Obszüchtung Pillnitzer Platz 3a, D-01326, Dresden, Germany

    Monika Höfer

  3. Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apdo. 4084, 14080, Córdoba, Spain

    Antonio Martin

Authors
  1. María Angeles Bueno
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Beatriz Pintos
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Monika Höfer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Antonio Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to María Angeles Bueno.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bueno, M.A., Pintos, B., Höfer, M. et al. Pro-embryos induction from Olea europaea L. isolated microspore culture. Acta Physiol Plant 27, 695–701 (2005). https://doi.org/10.1007/s11738-005-0073-8

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-005-0073-8

Key words

Search

Navigation