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Somatic embryogenesis and plant regeneration through leaf culture in Arachis glabrata (Leguminosae)

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Abstract

Plants of two accessions of Arachis glabrata were regenerated via somatic embryogenesis. Embryogenic calli were initiated from leaflet explants on Murashige and Skoog medium supplemented with picloram alone or picloram in combination with 6-benzylaminopurine. Leaflets of accession A6138 induced the highest percentage of somatic embryos in media composed of 10 mg dm−3 and 15 mg dm−3 picloram. In contrast, 5 mg dm−3 picloram with 0.1 mg dm−3 6-benzylaminopurine was one of the most effective combinations in accession AF385. MS medium supplemented with 2 g dm−3 activated charcoal (AC) used for 30 days was the most effective for embryo maturation. After 20 days of culture on MS medium devoid of growth regulators, 6 % of embryos converted into plantlets in accession A6138.

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Abbreviations

AC:

activated charcoal

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

MS:

Murashige and Skoog (1962) medium

NAA:

α-naphthaleneacetic acid

PIC:

picloram-(4-amino-3,5,6-trichloropicolinic acid)

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Authors and Affiliations

  1. Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste (IBONE), Casilla de Correos 209, 3400, Corrientes, Argentina

    María Laura Vidoz, Hebe Yolanda Rey, Ana María Gonzalez & Luis Amado Mroginski

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  1. María Laura Vidoz
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  2. Hebe Yolanda Rey
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  3. Ana María Gonzalez
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  4. Luis Amado Mroginski
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Correspondence to María Laura Vidoz.

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Vidoz, M.L., Rey, H.Y., Gonzalez, A.M. et al. Somatic embryogenesis and plant regeneration through leaf culture in Arachis glabrata (Leguminosae) . Acta Physiol Plant 26, 59–66 (2004). https://doi.org/10.1007/s11738-004-0045-4

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  • DOI: https://doi.org/10.1007/s11738-004-0045-4

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