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Light-enhanced dark respiration in leaves and mesophyll protoplasts of pea in relation to photorespiration, respiration and some metabolites content

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Abstract

The respiration rate of leaves and mesophyll protoplasts of pea (Pisum sativum L.), from plants which were previously kept in darkness for 24 h was doubled following a period of photosynthesis at ambient level of O2 (21 %), whereas the low level of O2 (1 % and 4 % for leaves and protoplasts, respectively) reduced this light-enhanced dark respiration (LEDR) to the rate as noted before the illumination. Similarly to respiration rate, the oxygen at used concentrations had no effect on the ATP/ADP ratio in the dark-treated leaves. However, the ATP/ADP ratio in leaves photosynthesizing at 21 % O2 was higher (up to 40 %, dependence on CO2 concentration in the range 40–1600 1 dm−3) than in those photosynthesizing at 1 % O2 or darkened at air (21 % O2). Also, at 1 % O2 the accumulation of malate was suppressed (by about 40 %), to a value noted for leaves darkened at 21 % O2. The dark-treatment of leaves reduced the ability of isolated mitochondria to oxidize glycine (by about twofold) and succinate, but not malate. Mitochondria from both the light- and dark-treated leaves did not differ in qualitative composition of free amino acids, however, there were significant quantitative differences especially with respect to aspartate, alanine, glutamate and major intermediates of the photorespiratory pathway (glycine, serine). Our results suggest that accumulation of photorespiratory and respiratory metabolites in pea leaves during photosynthesis at 1 % O2 is reduced, hence the suppression of postillumination respiration rate.

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Abbreviations

BSA:

bovine serinum albumin

EDTA:

ethylenediaminetetra acetic acid

GDC:

glycine decarboxylase

HEPES:

N-(2-hydroxyethyl)piperazine-N′-2(-ethanesulfonic acid)

LEDR:

light-enhanced dark respiration

MDH:

malate dehydrogenase

ME:

malic enzyme

MOPS:

3-(N-morpholino)propane sulfonic acid)

PDC:

pyruvate dehydrogenase

PFD:

photosynthetic photon flux density

PVP:

polyvinylpyrrolidone

RCR:

respiratory control rate

TCA:

tricarboxylic acid cycle

Tris:

tri(hydroxymethyl)methyloamine

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Authors and Affiliations

  1. Department of Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warszawa, Poland

    Eugeniusz Parys, Elżbieta Romanowska & Maria Siedlecka

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  1. Eugeniusz Parys
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  2. Elżbieta Romanowska
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  3. Maria Siedlecka
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Correspondence to Eugeniusz Parys.

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Parys, E., Romanowska, E. & Siedlecka, M. Light-enhanced dark respiration in leaves and mesophyll protoplasts of pea in relation to photorespiration, respiration and some metabolites content. Acta Physiol Plant 26, 37–46 (2004). https://doi.org/10.1007/s11738-004-0042-7

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  • DOI: https://doi.org/10.1007/s11738-004-0042-7

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