Skip to main content
SpringerLink
Log in

Methyl jasmonate stimulates the formation and the accumulation of anthocyanin in Kalanchoe blossfeldiana

  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Methyl jasmonate (JA-Me) at concentrations of 0.1, 0.5 and 1.0 % (w/w) greatly stimulated anthocyanins accumulation in shoots of young plants of Kalanchoe blossfeldiana when it was applied around the stem as a lanolin paste. Stimulatory effect of JA-Me was evidently observed as early as two days after treatment. Anthocyanins were formed in the main and lateral shoots, including petioles, both below and above portions of the treatment. When leaves were removed from the plant, almost no anthocyanin formation was observed. It should be mentioned that leaves are necessary for the anthocyanin accumulation in stems induced by JA-Me.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aerts R.J., Gisi D., De Carolis E., De Luca V., Banmann T.W., 1994. Methyl jasmonate vapor increases the developmentally controlled synthesis of alkaloids in Catharanthus and Cinchona seedlings. Plant J., 5: 635–643.

    Article  CAS  Google Scholar 

  • Blechert R.P., Brodshelm W., Hoylder S., Kammerer L., Kutchan T.M., Mueller M.J., Xia Z.Q., Zenk M.H., 1995. The octadecanoic pathway: Signal molecules for the regulation of secondary pathways. Proc. Natl. Acad. Sci. USA, 92: 4099–4105.

    Article  PubMed  CAS  Google Scholar 

  • Brulfert J., Guclu S., Taybi T., Pierre J.-N., 1993. Enzymatic responses to water-stress in detached leaves of the CAM plant Kalanchoe blossfeldiana Poelln. Plant Physiol. Biochem., 31: 491–497.

    CAS  Google Scholar 

  • Creelman R.A., Mullet J.E., 1995. Jasmonic acid distribution and action in plants: Regulation during development and response to biotic and abiotic stress. Proc. Natl. Acad. Sci. USA, 92: 4114–4119.

    Article  PubMed  CAS  Google Scholar 

  • Creelman R.A., Tierney M.L., Mullet J.E., 1992. Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. Proc. Natl. Acad. Sci. USA, 89: 4938–4941.

    Article  PubMed  CAS  Google Scholar 

  • Dittrich H., Kutchan T., Zenk M.H., 1992. The jasmonate precursor, 12-oxo-phytodienoic acid, induces phytoalexin synthesis in Petroselinum hortense cell cultures. FEBS Lett., 309: 33–36.

    Article  PubMed  CAS  Google Scholar 

  • Fang Y., Smith M.A.L., Pepin M.-F., 1999. Effects of exogenous methyl jasmonate in elicited anthocyanin-producing cell cultures of ohelo (Vaccinum pahalae). In Vitro Cell Dev. Biol.-Plant, 35: 106–113.

    Article  CAS  Google Scholar 

  • Feys B.J.F., Benedetti C.E., Penfold C.N., Turner J.G., 1994. Arabidopsis mutants selected for resistance to the phytotoxin coronatine are male sterile, insensitive to methyl jasmonate, and resistant to a bacterial pathogens. Plant Cell, 6: 751–759.

    Article  PubMed  CAS  Google Scholar 

  • Franceschi V.R., Grimes H.D., 1991. Induction of soybean vegetative proteins and anthocyanin by low-level atmospheric methyl jasmonate. Proc. Natl. Acad. Sci. USA, 88: 6745–6749.

    Article  PubMed  CAS  Google Scholar 

  • Groenewald E.G., Van der Westhuizen A.J., 1997. Prostaglandins and related substances in plants. Bot. Rev., 63: 199–220.

    Article  Google Scholar 

  • Groenewald E.G., Van der Westhuizen A.J., 2001. The flowering stimulus and possible involvement of prostaglandins in flowering of Pharbitis nil. S. Afr. J. Bot., 97: 313–317.

    CAS  Google Scholar 

  • Groenewald E.G., Visser J.H., 1974. The effect of certain inhibitors of prostaglandin biosynthesis of flowering Pharbitis nil. Z. Pflanzenphysiol., 71: 67–70.

    CAS  Google Scholar 

  • Groenewald E.G., Visser J.H., 1978. The effect of arachidonic acid, prostaglandins and inhibitors of prostaglandin synthetase, on the flowering of excised Pharbitis nil shoot apices under different photoperiods. Z. Pflanzenphysiol., 88: 423–429.

    CAS  Google Scholar 

  • Groenewald E.G., Visser J.H., Grobbelaar N., 1983. The occurrence of prostaglandins (PG) F2a in Pharbitis nil grown under short days or long days. S. Afr. J. Bot., 2: 82.

    Google Scholar 

  • Gundlach H., Muller M.J., Kutchan T.M., Zenk M.H., 1992. Jasmonic acid is a signal transducer in elicitor-induced plant cell culture. Proc. Natl. Acad. Sci. USA, 89: 2389–2393.

    Article  PubMed  CAS  Google Scholar 

  • Hamberg M., Gardner H.G., 1992. Oxylipin pathway to jasmonates: biochemistry and biological significance. Biochim. Biophys. Acta, 1165: 1–18.

    PubMed  CAS  Google Scholar 

  • Hayashi T., Konishi K., 1991. Inflorescence development and composition of Kalanchoe (Kalanchoe blossfeldiana Poelln.). J. Japan. Soc. Hort. Sci., 60: 167–173.

    Article  Google Scholar 

  • Janistyn B., 1982. Gas chromatographic-mass spectroscopic identification of prostaglandin F2a in flowering Kalanchoe blossfeldiana v. Poelln. Planta, 154: 485–487.

    Article  CAS  Google Scholar 

  • Mizukami H., Tabira Y., Ellis B.E., 1993. Methyl jasmonate-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension culture. Plant Cell Reports, 12: 706–709.

    Article  CAS  Google Scholar 

  • Mueller M.J., Brodschelm W., Spannagl E., Zenk M.H., 1993. Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid. Proc. Natl. Acad. Sci. USA, 90: 7490–7494.

    Article  PubMed  CAS  Google Scholar 

  • Murofushi N., Yamane H., Sakagami Y., Imaseki H., Kamiya Y., Iwamura H., Hirai N., Tsuji H., Yokota T., Ueda J., 1999. Plant Hormones. In: Comprehensive Natural Products Chemistry (Editors: Derek Barton, Sir Koji Nakanishi, Executive Editor: Otto Meth-Cohn), Vol. 8, Natural products, Pheromones, Plant hormones, and Aspects of Ecology (Volume Editor: Kenji Mori), Elsevier, Amsterdam, pp. 19–136.

    Google Scholar 

  • Neyland M., Ng Y.L., Thimann K.V., 1963. Formation of anthocyanin in leaves of Kalanchoe blossfeldiana — a photoperiodic response. Plant Physiol., 38: 447–451.

    Article  PubMed  CAS  Google Scholar 

  • Noijri H., Suguimori M., Yamane H., Nishimura Y., Yamada A., Shibuya N., Kodama O., Murofushi N., Omari T., 1996. Involvement of jasmonic acid in elicitor-induced phytoalexin production in suspension-cultured rice cells. Pant Physiol., 110: 387–392.

    Google Scholar 

  • Perez A.G., Sanz C., Olias R., Olias J.M., 1997. Effect of methyl jasmonate on in vitro strawberry ripening. J. Agric. Food Chem., 45: 3733–3737.

    Article  CAS  Google Scholar 

  • Saniewski M., 1989. Występowanie i aktywność fizjologiczna prostaglandyn w roślinach. Kosmos, 38: 211–223.

    Google Scholar 

  • Saniewski M., 1997. The role of jasmonates in ethylene biosynthesis. In: A.K. Kanellis, C. Chang, H. Kende, D. Grierson (eds.) Biology and Biotechnology of the Plant Hormone Ethylene, Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 39–45.

    Google Scholar 

  • Saniewski M., Miszczak A., Kawa-Miszczak L., Węgrzynowicz-Lesiak E., Miyamoto K., Ueda J., 1998. Effect of methyl jasmonate on anthocyanin accumulation, ethylene production, and CO2 evolution in uncooled and cooled tulip bulbs. J. Plant Growth Regul., 17: 33–37.

    Article  CAS  Google Scholar 

  • Saniewski M., Ueda J., Miyamoto K., 1999. Interaction of ethylene with jasmonates in the regulation of some physiological processes in plants. In: A.K. Kanellis, C. Chang, H. Klee, A.B. Bleecker, J.C. Pech, D. Grierson (eds.) Biology and Biotechnology of the Plant Hormone Ethylene II, Kluwer Academic Publishers, Dordrecht, Boston, pp. 173–180.

    Google Scholar 

  • Saniewski M., Ueda J., Miyamoto K., 2000. Methyl jasmonate induces the formation of secondary abscission zone in stem of Bryophyllum calycinum Salisb. Acta Physiol. Plant., 22: 17–23.

    Article  CAS  Google Scholar 

  • Saniewski M., Ueda J., Miyamoto K., 2002. Relationships between jasmonates and auxin in regulation of some physiological processes in higher plants. Acta Physiol. Plant., 24: 211–220.

    Article  CAS  Google Scholar 

  • Saniewski M., Węgrzynowicz-Lesiak E., 1995. Methyl jasmonate-induced leaf abscission in Kalanchoe blossfeldiana. Acta Hortic., 394: 315–324.

    CAS  Google Scholar 

  • Seo H.S., Song J.T., Cheong J.-J., Lee Y.-W., Hwang I., Choi Y.D., 2001. Jasmonic acid carboxyl methyltransferase: A key enzyme for jasmonate-regulated responses. Proc. Natl. Acad. Sci. USA, 98: 4788–4793.

    Article  PubMed  CAS  Google Scholar 

  • Swain T., 1965. Analytical methods for flavonoids. Chapter 19. In: Biochemistry of Plant Pigments. T.W. Goodwin (ed)., Academic Press, London, New York, pp. 533–549.

    Google Scholar 

  • Tamari G., Borochov A., Atzorn R., Weiss D., 1995. Methyl jasmonate induces pigmentation and flavonoid gene expression in petunia corollas: A possible role in wound response. Physiol. Plant., 94: 45–50.

    Article  CAS  Google Scholar 

  • Ueda J., Kato J., 1980. Isolation and identification of a senescence-promoting substances from wormwood (Artemisia absinthium L.) Plant Physiol., 66: 246–249.

    PubMed  CAS  Google Scholar 

  • Weiler E.W., 1997. Octadecanoid-mediated signal transduction in higher plants. Naturwissenschaften, 84: 340–349.

    Article  CAS  Google Scholar 

  • Zhang W., Curtin C., Kikuchi M., Franco C., 2002. Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures. Plant Sci., 162: 459–468.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Institute of Pomology and Floriculture, Pomologiczna 18, 96-100, Skierniewice, Poland

    Marian Saniewski

  2. Research Institute of Vegetable Crops, Konstytucji 3 Maja 1/3, 96-100, Skierniewice, Poland

    Marcin Horbowicz

  3. Botanical Garden - Center for Biological Diversity Conservation of the Polish Academy of Sciences, Prawdziwka 2, 02-973, Warszawa, Poland

    Marian Saniewski & Jerzy Puchalski

  4. College of Integrated Arts and Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, 599-8531, Sakai, Osaka, Japan

    Junichi Ueda

Authors
  1. Marian Saniewski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marcin Horbowicz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jerzy Puchalski
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Junichi Ueda
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saniewski, M., Horbowicz, M., Puchalski, J. et al. Methyl jasmonate stimulates the formation and the accumulation of anthocyanin in Kalanchoe blossfeldiana . Acta Physiol Plant 25, 143–149 (2003). https://doi.org/10.1007/s11738-003-0047-7

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-003-0047-7

Key words

Search

Navigation