Abstract
Protocols elaborated with the objective of achieving valuable material for selection procedure of variants with virusresistance traits in tomato genotypes are presented. Preliminary results are demonstrated in the domain of testing for variability in somaclones obtained through indirect adventitous organogenesis initiated on leaf explants of cultivated tomato (Lycopersicon esculentum Mill.). Somaclones were grown in greenhouse conditions and variation of their symptoms upon infection with tomato mosaic (ToMV) or cucumber mosaic (CMV) respectively was observed. Tests for resistance to the local isolates of the above cited viruses were performed using enzyme linked immunosorbent assay and back inoculation onto diagnostic plants. Screening data are presented. Desirable variants were selected from cultivars ‘Moneymaker’, ‘Potentat’ and ‘Rutgers’. Some of the ‘Moneymaker’ somaclones exhibited increased tolerance to cucumber mosaic virus, a few seemed to be even fully resistant though most were susceptible as donor plants. The most favourable somaclonal lines are actually further tested and monitored for changes in horticultural characteristics. The described procedure of searching for resistance trait in specific pathogen-free (SPF) plants regenerated from infected tissue looks promising and thus can serve as aid in attaining appropriate objectives of breeding programme.
Additionaly experiments were initiated to obtain somaclones from cultivars ‘Beta’, ‘Krakus’ and Stevens Rodade hybrid via regeneration of isolated protoplasts. To this end the callus stage was obtained from all donors.
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Abbreviations
- BA:
-
6-benzylaminopurine
- MES:
-
2-[N-Morpholino]ethane-sulfonic acid
- CMV:
-
cucumber mosaic virus
- ToMV:
-
tomato mosaic virus
- DAS ELISA:
-
double antibody sandwich procedure of enzyme-linked immunosorbent assay
- SPF plants:
-
specific pathogen-free plants
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Hanus-Fajerska, E., Lech, M., Pindel, A. et al. Selection for virus resistance in tomato exposed to tissue culture procedures. Acta Physiol Plant 22, 317–324 (2000). https://doi.org/10.1007/s11738-000-0045-y
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DOI: https://doi.org/10.1007/s11738-000-0045-y