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Screening Luffa germplasm and advanced breeding lines for resistance to Tomato leaf curl New Delhi virus

  • Viral and Viroid Diseases
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Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

The two cultivated Luffa species can be severely infected by Tomato leaf curl New Delhi virus (ToLCNDV) with up to 100% yield loss. Here, 52 Luffa genotypes were screened for ToLCNDV resistance after natural field infection. Mean vulnerability index (VI) ranged from 0.00 to 75.33; genotypes IIHR-137 and IIHR-138 had no symptoms (VI 0), 16 genotypes were resistant (VI 0–25), 15 were moderately resistant (VI 26–50), and 19 were moderate to susceptible (VI > 50). Ten of the most resistant genotypes and five susceptible checks were then challenge-inoculated using whiteflies or sap in an insect-proof net house; only IIHR-137 [L. cylindrica (L.) Roem.] was symptomless (VI 0.00), and 3–5% of plants of IIHR-138 [L. cylindrica (L.) Roem.] and IIHR-Sel-1 [L. acutangula (L.) Roxb.] had only mild symptoms; genotype Arka Prasan was most susceptible (VI 80.96). Asymptomatic plants were confirmed as infected using polymerase chain reaction. Susceptible genotypes rapidly developed leaf curling, then a severe mosaic 10 days post-inoculation. The resistant inbred lines identified are good candidates for a breeding program for ToLCNDV-resistant cultivars.

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Acknowledgements

The authors thank Dr A. D. Munshi for providing seeds of sponge gourd lines DSG-6 and DSG-7. The first author gratefully acknowledges Dr M. K. Reddy for his valuable guidance.

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Authors and Affiliations

  1. Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Bengaluru, Karnataka, 560089, India

    Manpreet Kaur, B. Varalakshmi & M. Pitchaimuthu

  2. Division of Plant Pathology, ICAR-Indian Institute of Horticultural Research, Bengaluru, Karnataka, 560089, India

    B. Mahesha

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  1. Manpreet Kaur
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  2. B. Varalakshmi
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  3. M. Pitchaimuthu
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  4. B. Mahesha
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Correspondence to Manpreet Kaur.

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Kaur, M., Varalakshmi, B., Pitchaimuthu, M. et al. Screening Luffa germplasm and advanced breeding lines for resistance to Tomato leaf curl New Delhi virus. J Gen Plant Pathol 87, 287–294 (2021). https://doi.org/10.1007/s10327-021-01010-z

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