Abstract
High fluorescence quantum yield (QY), excellent fluorescence stability, and low toxicity are essential for a good cellular imaging fluorescent probe. Green-emissive carbon quantum dots (CQDs) with many advantages, such as unique fluorescence properties, anti-photobleaching, low toxicity, fine biocompatibility and high penetration depth in tissues, have been considered as a potential candidate in cell imaging fluorescent probes. Herein, N, S-codoped green-emissive CQDs (QY = 64.03%) were synthesized by the one-step hydrothermal method, with m-phenylenediamine as the carbon and nitrogen source, and L-cysteine as the nitrogen and sulfur dopant, under the optimum condition of 200 °C reaction for 2 h. Their luminescence was found to originate from the surface state. In light of the satisfactory photobleaching resistance and the low cytotoxicity, CQDs were used as a cell imaging probe for HeLa cell imaging. The results clearly indicate that cells can be labeled with CQDs, which can not only enter the cytoplasm, but also enter the nucleus through the nuclear pore, showing their broad application prospect in the field of cell imaging.
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References
Yuan F, Yuan T, Sui L, et al. Engineering triangular carbon quantum dots with unprecedented narrow bandwidth emission for multicolored LEDs. Nature Communications, 2018, 9(1): 2249
Yang D X, Qu D, Miao X, et al. TiO2 sensitized by red-, green-, blue-emissive carbon dots for enhanced H2 production. Rare Metals, 2019, 38(5): 404–412
Huang S, Yang E, Yao J, et al. Red emission nitrogen, boron, sulfur co-doped carbon dots for “on-off-on” fluorescent mode detection of Ag+ ions and l-cysteine in complex biological fluids and living cells. Analytica Chimica Acta, 2018, 1035: 192–202
Song Y, Yan X, Li Z, et al. Highly photoluminescent carbon dots derived from linseed and their applications in cellular imaging and sensing. Journal of Materials Chemistry B: Materials for Biology and Medicine, 2018, 6(19): 3181–3187
Chen W F, Li D J, Tian L, et al. Synthesis of graphene quantum dots from natural polymer starch for cell imaging. Green Chemistry, 2018, 20(19): 4438–4442
Liu Y B, Chao D Y, Zhou L, et al. Yellow emissive carbon dots with quantum yield up to 68.6% from manganese ions. Carbon, 2018, 135: 253–259
Cheng Y, Li C, Mu R, et al. Dynamically long-term imaging of cellular RNA by fluorescent carbon dots with surface isoquinoline moieties and amines. Analytical Chemistry, 2018, 90(19): 11358–11365
Gao W L, Ma Y M, Zhou Y M, et al. High photoluminescent nitrogen-doped carbon dots with unique double wavelength fluorescence emission for cell imaging. Materials Letters, 2018, 216: 84–87
Khan W U, Wang D, Wang Y. Highly green emissive nitrogen-doped carbon dots with excellent thermal stability for bioimaging and solid-state LED. Inorganic Chemistry, 2018, 57(24): 15229–15239
Li H, Shao F Q, Huang H, et al. Eco-friendly and rapid microwave synthesis of green fluorescent graphitic carbon nitride quantum dots for vitro bioimaging. Sensors and Actuators B: Chemical, 2016, 226: 506–511
Zheng J, Xie Y, Wei Y, et al. An efficient synthesis and photoelectric properties of green carbon quantum dots with high fluorescent quantum yield. Nanomaterials, 2020, 10(1): 82
Yuan F, Wang Z, Li X, et al. Bright multicolor bandgap fluorescent carbon quantum dots for electroluminescent light-emitting diodes. Advanced Materials, 2017, 29(3): 1604436
Yuan B, Guan S, Sun X, et al. Highly efficient carbon dots with reversibly switchable green-red emissions for trichromatic white light-emitting diodes. ACS Applied Materials & Interfaces, 2018, 10(18): 16005–16014
Xu J, Wang C L, Li H Z, et al. Synthesis of green-emitting carbon quantum dots with double carbon sources and their application as a fluorescent probe for selective detection of Cu2+ ions. RSC Advances, 2020, 10(5): 2536–2544
Guo Y, Zhang J, Zhang W, et al. Green fluorescent carbon quantum dots functionalized with polyethyleneimine, and their application to aptamer-based determination of thrombin and ATP. Mikrochimica Acta, 2019, 186(11): 717
Liang J Y, Han L, Liu S G, et al. Green fluorescent carbon quantum dots as a label-free probe for rapid and sensitive detection of hematin. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2019, 212: 167–172
Moniruzzaman M, Kim J. N-doped carbon dots with tunable emission for multifaceted application: Solvatochromism, moisture sensing, pH sensing, and solid state multicolor lighting. Sensors and Actuators B: Chemical, 2019, 295: 12–21
Sahu S, Behera B, Maiti T K, et al. Simple one-step synthesis of highly luminescent carbon dots from orange juice: Application as excellent bio-imaging agents. Chemical Communications, 2012, 48(70): 8835–8837
Huang S, Yang E, Liu Y, et al. Low-temperature rapid synthesis of nitrogen and phosphorus dual-doped carbon dots for multicolor cellular imaging and hemoglobin probing in human blood. Sensors and Actuators B: Chemical, 2018, 265: 326–334
Gao Z H, Lin Z Z, Chen X M, et al. Carbon dots-based fluorescent probe for trace Hg2+ detection in water sample. Sensors and Actuators B: Chemical, 2016, 222: 965–971
Wang J L, Zhang F, Wang Y L, et al. Efficient resistance against solid-state quenching of carbon dots towards white light emitting diodes by physical embedding into silica. Carbon, 2018, 126: 426–436
Song Z Q, Quan F Y, Xu Y H, et al. Multifunctional N, S co-doped carbon quantum dots with pH- and thermo-dependent switchable fluorescent properties and highly selective detection of glutathione. Carbon, 2016, 104: 169–178
Shu Y, Lu J, Mao Q X, et al. Ionic liquid mediated organophilic carbon dots for drug delivery and bioimaging. Carbon, 2017, 114: 324–333
Liu J, Li D, Zhang K, et al. One-step hydrothermal synthesis of nitrogen-doped conjugated carbonized polymer dots with 31% efficient red emission for in vivo imaging. Small, 2018, 14(15): 1703919
Wang J, Wang S, Wei Y, et al. Rapid synthesis of nitrogen doped carbon dots with green fluorescent for bio-imaging. Optical Materials, 2019, 98: 109486
Hua X W, Bao Y W, Wu F G. Fluorescent carbon quantum dots with intrinsic nucleolus-targeting capability for nucleolus imaging and enhanced cytosolic and nuclear drug delivery. ACS Applied Materials & Interfaces, 2018, 10(13): 10664–10677
Li F, Li Y, Yang X, et al. Highly fluorescent chiral N-S-doped carbon dots from cysteine: Affecting cellular energy metabolism. Angewandte Chemie International Edition, 2018, 57(9): 2377–2382
Hu L, Li H, Liu C A, et al. Chiral evolution of carbon dots and the tuning of laccase activity. Nanoscale, 2018, 10(5): 2333–2340
Wang W, Lu Y C, Huang H, et al. Facile synthesis of N, S-codoped fluorescent carbon nanodots for fluorescent resonance energy transfer recognition of methotrexate with high sensitivity and selectivity. Biosensors & Bioelectronics, 2015, 64: 517–522
Yang J, Gao G, Zhang X, et al. Ultrasmall and photostable nanotheranostic agents based on carbon quantum dots passivated with polyamine-containing organosilane molecules. Nanoscale, 2017, 9(40): 15441–15452
Xu H B, Zhou S H, Xiao L L, et al. Fabrication of a nitrogen-doped graphene quantum dot from MOF-derived porous carbon and its application for highly selective fluorescence detection of Fe3+. Journal of Materials Chemistry C: Materials for Optical and Electronic Devices, 2015, 3(2): 291–297
Hou Y, Lu Q, Deng J, et al. One-pot electrochemical synthesis of functionalized fluorescent carbon dots and their selective sensing for mercury ion. Analytica Chimica Acta, 2015, 866: 69–74
Cui X, Zhu L, Wu J, et al. A fluorescent biosensor based on carbon dots-labeled oligodeoxyribonucleotide and graphene oxide for mercury(II) detection. Biosensors & Bioelectronics, 2015, 63: 506–512
Gao Y, Jiao Y, Lu W, et al. Carbon dots with red emission as a fluorescent and colorimeteric dual-readout probe for the detection of chromium(vi) and cysteine and its logic gate operation. Journal of Materials Chemistry B: Materials for Biology and Medicine, 2018, 6(38): 6099–6107
Ding H, Wei J S, Zhang P, et al. Solvent-controlled synthesis of highly luminescent carbon dots with a wide color gamut and narrowed emission peak widths. Small, 2018, 14(22): 1800612
Niu F S, Ying Y L, Hua X, et al. Electrochemically generated green-fluorescent N-doped carbon quantum dots for facile monitoring alkaline phosphatase activity based on the Fe3+-mediating on-off-on-off fluorescence principle. Carbon, 2018, 127: 340–348
Ding H, Yu S B, Wei J S, et al. Full-color light-emitting carbon dots with a surface-state-controlled luminescence mechanism. ACS Nano, 2016, 10(1): 484–491
Wang Y, Zhuang Q, Ni Y. Facile microwave-assisted solid-phase synthesis of highly fluorescent nitrogen-sulfur-codoped carbon quantum dots for cellular imaging applications. Chemistry: A European Journal, 2015, 21(37): 13004–13011
Guo L, Li L, Liu M, et al. Bottom-up preparation of nitrogen doped carbon quantum dots with green emission under microwave-assisted hydrothermal treatment and their biological imaging. Materials Science and Engineering C: Materials for Biological Applications, 2018, 84: 60–66
Liu Y, Duan W, Song W, et al. Red emission B, N, S-co-doped carbon dots for colorimetric and fluorescent dual mode detection of Fe3+ ions in complex biological fluids and living cells. ACS Applied Materials & Interfaces, 2017, 9(14): 1266–12672
Jung Y K, Shin E, Kim B S. Cell nucleus-targeting zwitterionic carbon dots. Scientific Reports, 2015, 5(1): 18807
Pandit S, Behera P, Sahoo J, et al. In situ synthesis of amino acid functionalized carbon dots with tunable properties and their biological applications. ACS Applied Bio Materials, 2019, 2(8): 3393–3403
Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant Nos. 51972221 and 51803148), Central Government Guides Local Science and Technology Development Projects (YDZX20201400001722), the Shanxi Provincial Excellent Talents Science and Technology Innovation Project (201805D211001), and the Natural Science Foundation of Shanxi Province (201901D211502 and 201901D211501).
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Wei, Y., Chen, L., Zhao, S. et al. Green-emissive carbon quantum dots with high fluorescence quantum yield: Preparation and cell imaging. Front. Mater. Sci. 15, 253–265 (2021). https://doi.org/10.1007/s11706-021-0544-x
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DOI: https://doi.org/10.1007/s11706-021-0544-x