Abstract
Adult central nervous system (CNS) tissue has a limited capacity to recover after trauma or disease. Recent medical cell therapy using polymeric biomaterialloaded stem cells with the capability of differentiation to specific neural population has directed focuses toward the recovery of CNS. Fibers that can provide topographical, biochemical and electrical cues would be attractive for directing the differentiation of stem cells into electro-responsive cells such as neuronal cells. Here we report on the fabrication of an electrospun polypyrrole/polylactide composite nanofiber film that direct or determine the fate of mesenchymal stem cells (MSCs), via combination of aligned surface topography, and electrical stimulation (ES). The surface morphology, mechanical properties and electric properties of the film were characterized. Comparing with that on random surface film, expression of neurofilament-lowest and nestin of human umbilical cord mesenchymal stemcells (huMSCs) cultured on film with aligned surface topography and ES were obviously enhanced. These results suggest that aligned topography combining with ES facilitates the neurogenic differentiation of huMSCs and the aligned conductive film can act as a potential nerve scaffold.
Similar content being viewed by others
References
Liu X, Pi B, Wang H, et al. Self-assembling peptide nanofiber hydrogels for central nervous system regeneration. Frontiers of Materials Science, 2015, 9(1): 1–13
He J, Wang X M, Spector M, et al. Scaffolds for central nervous system tissue engineering. Frontiers of Materials Science, 2012, 6 (1): 1–25
Bagher Z, Ebrahimi-Barough S, Azami M, et al. Cellular activity of Wharton’s Jelly-derived mesenchymal stem cells on electrospun fibrous and solvent-cast film scaffolds. Journal of Biomedical Materials Research Part A, 2016, 104(1): 218–226
Irani S, Zandi M, Salamian N, et al. The study of P19 stem cell behavior on aligned oriented electrospun poly(lactic-co-glycolic acid) nano-fibers for neural tissue engineering. Polymers for Advanced Technologies, 2014, 25(5): 562–567
Wang X, He J, Wang Y, et al. Hyaluronic acid-based scaffold for central neural tissue engineering. Interface Focus, 2012, 2(3): 278–291
Lu P, Blesch A, Tuszynski M H. Induction of bone marrow stromal cells to neurons: differentiation, transdifferentiation, or artifact? Journal of Neuroscience Research, 2004, 77(2): 174–191
Wang Y, Yao S, Meng Q, et al. Gene expression profiling and mechanism study of neural stem cells response to surface chemistry. Regenerative Biomaterials, 2014, 1(1): 37–47
Liu X, Wang X, Wang X, et al. Functionalized self-assembling peptide nanofiber hydrogels mimic stem cell niche to control human adipose stem cell behavior in vitro. Acta Biomaterialia, 2013, 9(6): 6798–6805
Liu X, He J, Zhang S, et al. Adipose stem cells controlled by surface chemistry. Journal of Tissue Engineering and Regenerative Medicine, 2013, 7(2): 112–117
Liu X, Wang Y, He J, et al. Various fates of neuronal progenitor cells observed on several different chemical functional groups. Frontiers of Materials Science, 2011, 5(4): 358–366
Yao S L, Liu X, Wang X M, et al. Directing neural stem cell fate with biomaterial parameters for injured brain regeneration. Progress in Natural Science: Materials International, 2013, 23 (2): 103–112
Zhang J G, Qiu K X, Sun B B, et al. The aligned core–sheath nanofibers with electrical conductivity for neural tissue engineering. Journal of Materials Chemistry B: Materials for Biology and Medicine, 2014, 2(45): 7945–7954
Lanfer B, Hermann A, Kirsch M, et al. Directed growth of adult human white matter stem cell-derived neurons on aligned fibrillar collagen. Tissue Engineering Part A, 2010, 16(4): 1103–1113
Lim S H, Liu X Y, Song H, et al. The effect of nanofiber-guided cell alignment on the preferential differentiation of neural stem cells. Biomaterials, 2010, 31(34): 9031–9039
Çapkin M, Çakmak S, Kurt F O, et al. Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: comparison of neural differentiation of rat AdMSCs and BMSCs. Biomedical Materials, 2012, 7(4): 045013
Yao S, Liu X, Yu S, et al. Co-effects of matrix low elasticity and aligned topography on stem cell neurogenic differentiation and rapid neurite outgrowth. Nanoscale, 2016, 8(19): 10252–10265
Ghasemi-Mobarakeh L, Prabhakaran M P, Morshed M, et al. Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering. Journal of Tissue Engineering and Regenerative Medicine, 2011, 5(4): e17–e35
Park S J, Park J S, Yang H N, et al. Neurogenesis is induced by electrical stimulation of human mesenchymal stem cells cocultured with mature neuronal cells. Macromolecular Bioscience, 2015, 15(11): 1586–1594
Thrivikraman G, Madras G, Basu B. Intermittent electrical stimuli for guidance of human mesenchymal stem cell lineage commitment towards neural-like cells on electroconductive substrates. Biomaterials, 2014, 35(24): 6219–6235
Park J S, Yang H N, Woo D G, et al. Exogenous Nurr1 gene expression in electrically-stimulated human MSCs and the induction of neurogenesis. Biomaterials, 2012, 33(29): 7300–7308
Prabhakaran M P, Ghasemi-Mobarakeh L, Jin G, et al. Electrospun conducting polymer nanofibers and electrical stimulation of nerve stem cells. Journal of Bioscience and Bioengineering, 2011, 112(5): 501–507
Piacentini R, Ripoli C, Mezzogori D, et al. Extremely lowfrequency electromagnetic fields promote in vitro neurogenesis via upregulation of Cav1-channel activity. Journal of Cellular Physiology, 2008, 215(1): 129–139
Huang Y J, Wu H C, Tai N H, et al. Carbon nanotube rope with electrical stimulation promotes the differentiation and maturity of neural stem cells. Small, 2012, 8(18): 2869–2877
Gunewardene N, Dottori M, Nayagam B A. The convergence of cochlear implantation with induced pluripotent stem cell therapy. Stem Cell Reviews, 2012, 8(3): 741–754
Sauer H, Rahimi G, Hescheler J, et al. Effects of electrical fields on cardiomyocyte differentiation of embryonic stem cells. Journal of Cellular Biochemistry, 1999, 75(4): 710–723
Balint R, Cassidy N J, Cartmell S H. Electrical stimulation: a novel tool for tissue engineering. Tissue Engineering Part B: Reviews, 2013, 19(1): 48–57
Sheikh F A, Ju H W, Moon B M, et al. A comparative mechanical and biocompatibility study of poly(e-caprolactone), hybrid poly(e- caprolactone)-silk, and silk nanofibers by colloidal electrospinning technique for tissue engineering. Journal of Bioactive and Compatible Polymers, 2014, 29(5): 500–514
Zhang H L. Effects of electrospinning parameters on morphology and diameter of electrospun PLGA/MWNTs fibers and cytocompatibility in vitro. Journal of Bioactive and Compatible Polymers, 2011, 26(6): 590–606
Shi G, Rouabhia M, Wang Z, et al. A novel electrically conductive and biodegradable composite made of polypyrrole nanoparticles and polylactide. Biomaterials, 2004, 25(13): 2477–2488
Pelto J, Björninen M, Pälli A, et al. Novel polypyrrole-coated polylactide scaffolds enhance adipose stem cell proliferation and early osteogenic differentiation. Tissue Engineering Part A, 2013, 19(7–8): 882–892
Shi G, Zhang Z, Rouabhia M. The regulation of cell functions electrically using biodegradable polypyrrole–polylactide conductors. Biomaterials, 2008, 29(28): 3792–3798
Lee J Y, Bashur C A, Goldstein A S, et al. Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications. Biomaterials, 2009, 30(26): 4325–4335
Xie J, Macewan M R, Willerth S M, et al. Conductive core–sheath nanofibers and their potential application in neural tissue engineering. Advanced Functional Materials, 2009, 19(14): 2312–2318
Sudwilai T, Ng J J, Boonkrai C, et al. Polypyrrole-coated electrospun poly(lactic acid) fibrous scaffold: effects of coating on electrical conductivity and neural cell growth. Journal of Biomaterials Science: Polymer Edition, 2014, 25(12): 1240–1252
El Omar R, Beroud J, Stoltz J F, et al. Umbilical cord mesenchymal stem cells: the new gold standard for mesenchymal stem cell-based therapies? Tissue Engineering Part B: Reviews, 2014, 20(5): 523–544
Zheng R, Sun X. Influence of template agent and oxidant on morphology and electrical conductivity of polypyrrole nano particles. Polymer Materials Science and Engineering, 2012, 28 (12): 72–75, 80
Ghasemi-Mobarakeh L, Prabhakaran M P, Morshed M, et al. Electrical stimulation of nerve cells using conductive nanofibrous scaffolds for nerve tissue engineering. Tissue Engineering Part A, 2009, 15(11): 3605–3619
Chung TW, Liu D Z, Wang S Y, et al. Enhancement of the growth of human endothelial cells by surface roughness at nanometer scale. Biomaterials, 2003, 24(25): 4655–4661
Wu L P, You M L, Wang D Y, et al. Fabrication of carbon nanotube (CNT)/poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) nanocomposite films for human mesenchymal stem cell (hMSC) differentiation. Polymer Chemistry, 2013, 4 (16): 4490–4498
Yang A, Huang Z, Yin G, et al. Fabrication of aligned, porous and conductive fibers and their effects on cell adhesion and guidance. Colloids and Surfaces B: Biointerfaces, 2015, 134: 469–474
Au H T H, Cheng I, Chowdhury M F, et al. Interactive effects of surface topography and pulsatile electrical field stimulation on orientation and elongation of fibroblasts and cardiomyocytes. Biomaterials, 2007, 28(29): 4277–4293
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Zhou, J., Cheng, L., Sun, X. et al. Neurogenic differentiation of human umbilical cord mesenchymal stem cells on aligned electrospun polypyrrole/polylactide composite nanofibers with electrical stimulation. Front. Mater. Sci. 10, 260–269 (2016). https://doi.org/10.1007/s11706-016-0348-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11706-016-0348-6