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Surface molecularly imprinted polymers for solid-phase extraction of (–)-epigallocatechin gallate from toothpaste

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Abstract

Surface molecularly imprinted polymers (SMIPs) have been synthesized to selectively determine (–)-epigallocatechin gallate in aqueous media. SMIPs were prepared using a surface grafting copolymerization method on a functionalized silica gel modified with β-cyclodextrin and vinyl groups. The morphology and composition of the SMIPs were investigated by scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analysis. In addition, the molecular binding capacity, recognition properties and selectivity of the SMIPs were evaluated. The imprinted polymers were found to have a highly specific recognition and binding capacity for (–)-epigallocatechin gallate in aqueous media which is the result of the hydrophobic properties of the β-cyclodextrin and the hydrogen-bonding interactions of methacrylic acid. The SMIPs were successfully employed as solid-phase extraction adsorbents prior to the HPLC determination of (–)-epigallocatechin gallate in toothpaste. The HPLC analysis had a linear dynamic range of 0.5–50.0 μg·mL−1 with a correlation coefficient of 0.9998 and the recoveries ranged from 89.4% to 97.0% with relative standard deviations less than 4.8%. The limit of detection and limit of quantification were 0.17 and 0.33 μg·mL−1, respectively. The method provides a promising approach for the preparation of selective materials for the purification and determination of complex samples.

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  1. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, 310032, China

    Yunling Gao, Ying Hu & Kejian Yao

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  1. Yunling Gao
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  2. Ying Hu
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Gao, Y., Hu, Y. & Yao, K. Surface molecularly imprinted polymers for solid-phase extraction of (–)-epigallocatechin gallate from toothpaste. Front. Chem. Sci. Eng. 9, 467–478 (2015). https://doi.org/10.1007/s11705-015-1526-2

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  • DOI: https://doi.org/10.1007/s11705-015-1526-2

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