Abstract
Synthesis of nanoparticles using naturally occurring biomolecules has become the preferred method due to increased concerns over environmental degradation. In this study, the biosynthesis of manganese dioxide nanoparticles (MnO2NPs) and silver nanoparticles (AgNPs) using extracts and the biomolecule, aralia cerebroside, isolated from the medicinal plant species, Cussonia zuluensis Strey, was investigated. The size and morphology of nanoparticles observed using microscopic techniques indicated an average particle size of 7.43 nm (spherical and polydispersed) for AgNPs and a layer of thin film surrounding the particles, confirming the capping by biomolecules. AgNPs exhibited better antibacterial activity than MnO2NPs and were most active against Escherichia coli and Enterococcus faecalis. MnO2NPs presented as ultrathin nanoflakes with grainy morphology ranging from 11 to 29 nm when capped with biomolecules from the extract, and presented as nanospheres surrounded by nanosheets ranging from 6.99 to 16.57 nm when capped with aralia cerebroside. The radical scavenging activity was found to be MnO2NPs (extract) > MnO2NPs (cerebroside) > AgNPs (extract) > extract > cerebroside, and the ferric reducing antioxidant power was found to be cerebroside > extract > MnO2NPs (cerebroside) > MnO2NPs (extract) > AgNPs (extract). MnO2NPs exhibited better antioxidant activity than AgNPs with size and morphology of nanoparticles being influenced by the capping agent, which, in turn, influenced antioxidant activity as seen with MnO2NPs. This study confirms the significance of the metal or metal oxide core and capping biomolecules for targeted therapeutic activity of nanoparticles using the plant-mediated synthesis route.
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References
Ahmed Y, Rahman S, Akthar P, Islam F, Rahman M, Yaakob Z (2013) Isolation of steroids from n-hexane extract of the leaves of Saurauia roxburghii. Int Food Res J 20:2939–2943
Ahmed S, Saifullah Ahmad M, Swami BL, Ikram S (2016) Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. J Radiat Res Appl Sci 9:1–7. https://doi.org/10.1016/j.jrras.2015.06.006
Athar T, Topnani N, Hakeem A, Ahmed W (2012) Synthesis and characterization of MnO2 and CdO nanoparticles. Adv Sci Lett 7:39–42. https://doi.org/10.1166/asl.2012.2191
Bayoudh A, Etteyeb N, Sediri F (2016) Hydrothermal synthesis, physico-chemical characterization and electrochemical behavior of β-MnO2 nanorods. Am J Nanosci 2:1–7. https://doi.org/10.11648/j.ajn.20160201.11
Birgisson S, Saha D, Iversen B (2018) Formation mechanisms of nanocrystalline MnO2 polymorphs under hydrothermal conditions. Cryst Growth Des 18:827–838. https://doi.org/10.1021/acs.cgd.7b01304
Chen L, Luan G (2017) Morphology control of MnO2 nanoparticles: effect of P123 polymer in ethanol-water system. Chem Ind Chem Eng Q 23:245–249. https://doi.org/10.2298/ciceq160316038c
Chen X, Yan S, Wang N, Peng S, Wang C, Hong Q, Zhang X, Dai S (2017) Facile synthesis and characterization of ultrathin delta-MnO2 nanoflakes. RSC Adv 7:55734–55740. https://doi.org/10.1039/C7RA08962G
Cherian E, Rajan A, Baskar G (2016) Synthesis of manganese dioxide nanoparticles using co-precipitation method and its antimicrobial activity. Int J Mod Sci Technol 1:17–22
Corra S, Shoshan M, Wennemers H (2017) Peptide mediated formation of noble metal nanoparticles—controlling size and spatial arrangement. Curr Opin Chem Biol 40:138–144. https://doi.org/10.1016/j.cbpa.2017.09.005
De Villiers BJ, Van Vuuren SF, Van Zyl RL, Van Wyk BE (2010) Antimicrobial and antimalarial activity of Cussonia species (Araliaceae). J Ethnopharmacol 129:189–196. https://doi.org/10.1016/j.jep.2010.02.014
Deljou A, Goudarzi S (2016) Green extracellular synthesis of the silver nanoparticles using thermophilic Bacillus Sp. AZ1 and its antimicrobial activity against several human pathogenetic bacteria. Iran J Biotechnol 14:25–32. https://doi.org/10.15171/ijb.1259
Demirbas A, Büyükbezirci K, Celik C, Kislakci E, Karaagac Z, Gokturk E, Kati A, Cimen B, Yilmaz V, Ocsoy I (2019) Synthesis of long-term stable gold nanoparticles benefiting from red raspberry (Rubus idaeus), strawberry (Fragaria ananassa), and blackberry (Rubus fruticosus) extracts − gold ion complexation and investigation of reaction conditions. ACS Omega 4:18637–18644. https://doi.org/10.1021/acsomega.9b02469
Dogru E, Demirbas A, Altinsoy B, Duman F, Ocsoy I (2017) Formation of Matricaria chamomilla extract-incorporated Ag nanoparticles and size-dependent enhanced antimicrobial property. J Photochem Photobiol B 174:78–83. https://doi.org/10.1016/j.jphotobiol.2017.07.024
Ebede GR, Ndongo JT, Mbing JN, Kenfack HCM, Pegnyemb DE, Bochet CG (2019) Contortamide, a new anti-colon cancer cerebroside and other constituents from Tabernaemontana contorta Stapf (Apocynaceae). Nat Prod Res. https://doi.org/10.1080/14786419.2019.1636243
Elgorban A, El-Samawaty A, Yassin M, Sayed S, Adil S, Elhindi K, Bakri M, Khan M (2016) Antifungal silver nanoparticles: synthesis, characterization and biological evaluation. Biotechnol Biotechnol Equip 30:56–62. https://doi.org/10.1080/13102818.2015.1106339
Guilger-Casagrande M, Germano-Costa T, Pasquoto-Stigliani T, Fraceto L, de Lima R (2019) Biosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorum. Sci Rep 9:1–9. https://doi.org/10.1038/s41598-019-50871-0
Guzman M, Dille J, Godet S (2012) Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria. Nanomedicine 8:37–45. https://doi.org/10.1016/j.nano.2011.05.007
Haneefa MM, Jayandran M, Balasubramanian V (2017) Green synthesis characterization and antimicrobial activity evaluation of manganese oxide nanoparticles and comparative studies with salicylalchitosan functionalized nanoform. Asian J Pharm 11:65–74. https://doi.org/10.22377/ajp.v11i01.1045
Hankey A (2005) Cussonia transvaalensis Reyneke. pza.sanbi.org/cussonia-transvaalensis%0D. Accessed 18 Jul 2019
Hazarika M, Borah D, Bora P, Silva A, Das P (2017) Biogenic synthesis of palladium nanoparticles and their applications as catalyst and antimicrobial agent. PLoS ONE 12:1–19. https://doi.org/10.1371/journal.pone.0184936
Hoseinpour V, Souri M, Ghaemi N (2018) Green synthesis, characterisation, and photocatalytic activity of manganese dioxide nanoparticles. Micro Nano Lett 13:1560–1563. https://doi.org/10.1049/mnl.2018.5008
Iravani S (2011) Green synthesis of metal nanoparticles using plants. Green Chem 13:2638–2650. https://doi.org/10.1039/c1gc15386b
Jaganyi D, Altaf M, Wekesa I (2013) Synthesis and characterization of whisker-shaped MnO2 nanostructure at room temperature. Appl Nanosci 3:329–333. https://doi.org/10.1007/s13204-012-0135-3
Joshi N, Joshi E, Singh A (2020) Biological synthesis, characterisations and antimicrobial activities of manganese dioxide (MnO2) nanoparticles. Res J Pharm Technol 13:135–140. https://doi.org/10.5958/0974-360X.2020.00027.X
Julin S, Nummelin S, Kostiainen M, Linko V (2018) DNA nanostructure-directed assembly of metal nanoparticle superlattices. J Nanopart Res 20:1–11. https://doi.org/10.1007/s11051-018-4225-3
Kaczmarek H, Metzler M, Wegrzynowska-Drzymalska K (2016) Effect of stabilizer type on the physicochemical properties of poly (acrylic acid)/silver nanocomposites for biomedical applications. Polym Bull 73:2927–2945. https://doi.org/10.1007/s00289-016-1617-3
Kang SS, Kim JS, Xu YN, Kim YH (1999) Isolation of a new cerebroside from the root bark of Aralia elata. J Nat Prod 62:1059–1060. https://doi.org/10.1021/np990018r
Kedi P, Meva E, Kotsedi L, Nguemfo E, Ntoumba A, Ahmed N, Mohamed Dongmo A, Maaza M (2018) Eco-friendly synthesis, characterization, in vitro and in vivo anti-inflammatory activity of silver nanoparticle-mediated Selaginella myosurus aqueous extract. Int J Nanomed 13:8537–8548. https://doi.org/10.2147/IJN.S174530
Khan MZH, Tareq FK, Hossen MA, Roki MNAM (2018) Green synthesis and characterization of silver nanoparticles using Coriandrum savitum leaf extract. J Eng Sci Technol 13:158–166
Khatun M, Billah M, Quader A (2012) Sterols and sterol glucoside from Phyllanthus species. Dhaka Univ J Sci 60:5–10. https://doi.org/10.3329/dujs.v60i1.10327
Kumar BMP, Shivaprasad KH, Raveendra RS, Krishna RH, Karikkat S, Nagabhushana BM (2014) Preparation of MnO2 nanoparticles for the adsorption of environmentally hazardous malachite green dye. Int J Appl Innov Eng Manag 3:102–106
Lee JM, Lee DG, Lee KH, Cho SH, Nam K, Lee S (2013) Isolation and identification of phytochemical constituents from the fruits of Acanthopanax senticosus. Afr J Pharm Pharmacol 7:294–301. https://doi.org/10.5897/AJPP12.898
Leng Y, Fu L, Li B, Xu X, Xing X, He J, Song Y, Leng C, Guo Y, Ji X, Lu Z (2016) Protein-directed synthesis of highly monodispersed, spherical gold nanoparticles and their applications in multidimensional sensing. Sci Rep 6:1–11. https://doi.org/10.1038/srep28900
Ling T, Xia T, Wan X, Li D, Wei X (2006) Cerebrosides from the roots of Serratula chinensis. Molecules 11:677–683. https://doi.org/10.3390/11090677
Liu Z, Xu K, Sun H, Yin S (2015) One-step synthesis of single-layer MnO2 nanosheets with multi-role sodium dodecyl sulfate for high-performance pseudocapacitors. Small 11:2182–2191. https://doi.org/10.1002/smll.201402222
Logeswari P, Silambarasan S, Abraham J (2015) Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property. J Saudi Chem Soc 19:311–317. https://doi.org/10.1016/j.jscs.2012.04.007
Loo Y, Rukayadi Y, Nor-Khaizura M, Kuan C, Chieng B, Nishibuchi M, Radu S (2018) In vitro antimicrobial activity of green synthesized silver nanoparticles against selected gram-negative foodborne pathogens. Front Microbiol 9:1–7. https://doi.org/10.3389/fmicb.2018.01555
Loza K, Epple M (2018) Silver nanoparticles in complex media: an easy procedure to discriminate between metallic silver nanoparticles, reprecipitated silver chloride, and dissolved silver species. RSC Adv 8:24386–24391. https://doi.org/10.1039/C8RA04500C
Luo Y (2007) Preparation of MnO2 nanoparticles by directly mixing potassium permanganate and polyelectrolyte aqueous solutions. Mater Lett 61:1893–1895. https://doi.org/10.1016/j.matlet.2006.07.165
Makarov VV, Love AJ, Sinitsyna OV, Makarova SS, Yaminsky IV, Taliansky ME, Kalinina NO (2014) “Green” nanotechnologies: synthesis of metal nanoparticles using plants. Acta Naturae 6:35–44. https://doi.org/10.4135/9781452231631.n3
Malik P, Shankar R, Malik V, Sharma N, Mukherjee TK (2014) Green chemistry based benign routes for nanoparticle synthesis. J Nanoparticles 2014:1–14. https://doi.org/10.1155/2014/302429
Moon J-H, Munakata H, Kajihara K, Kanamura K (2013) Hydrothermal synthesis of manganese dioxide nanoparticles as cathode material for rechargeable batteries. Electrochemistry 81:2–6. https://doi.org/10.5796/electrochemistry.81.2
Moon SA, Salunke BK, Alkotaini B, Sathiyamoorthi E, Beom Soo K (2015) Biological synthesis of manganese dioxide nanoparticles by Kalopanax pictus plant extract. IET Nanobiotechnol 9:220–225. https://doi.org/10.1049/iet-nbt.2014.0051
Nasrollahzadeh M, Yek S, Motahharifar N, Gorab M (2019) Recent developments in the plant-mediated green synthesis of Ag-based nanoparticles for environmental and catalytic applications. Chem Rec 19:1–45. https://doi.org/10.1002/tcr.201800202
Nitie-Kang F, Onguéne PA, Lifongo LL, Ndom JC, Sippl W, Mbaze LM (2014) The potential of anti-malarial compounds derived from African medicinal plants, part II: a pharmacological evaluation of non-alkaloids and non-terpenoids. Malar J 13:1–20. https://doi.org/10.1186/1475-2875-13-81
Ocsy I, Demirbas A, McLamore E, Altinsoy B, Ildiz N, Baldemir A (2017) Green synthesis with incorporated hydrothermal approaches for silver nanoparticles formation and enhanced antimicrobial activity against bacterial and fungal pathogens. J Mol Liq 238:263–269. https://doi.org/10.1016/j.molliq.2017.05.012
Oladimeji AO, Oladosu IA, Jabeen A, Faheem A, Mesaik MA, Ali MS (2017) Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea. Pharm Biol 55:2240–2247. https://doi.org/10.1080/13880209.2017.1400078
Parveen K, Banse V, Ledwani L (2016) Green synthesis of nanoparticles: Their advantages and disadvantages. In: Sharma NN, Gaol FL, Akthar J (eds) 2nd International Conference on Emerging Technologies: Micro to Nano 2015 (ETMN-2015). AIP Publishing, Rajasthan, pp 1–7
Perni S, Hakala V, Prokopovich P (2013) Biogenic synthesis of antimicrobial silver nanoparticles capped with L-cysteine. Colloids Surf A 460:219–224. https://doi.org/10.1016/j.colsurfa.2013.09.034
Saeed N, Khan MR, Shabbir M (2012) Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med 12:1–12. https://doi.org/10.1186/1472-6882-12-221
Sana S, Dogiparthi L (2018) Green synthesis of silver nanoparticles using Givotia moluccana leaf extract and evaluation of their antimicrobial activity. Mater Lett 226:47–51. https://doi.org/10.1016/j.matlet.2018.05.009
Sangaonkar GM, Pawar KD (2018) Garcinia indica mediated biogenic synthesis of silver nanoparticles with antibacterial and antioxidant activities. Colloids Surf B Biointerfaces 164:210–217. https://doi.org/10.1016/j.colsurfb.2018.01.044
Seoudi R, Shabaka A, El Sayed ZA, Anis B (2011) Effect of stabilizing agent on the morphology and optical properties of silver nanoparticles. Phys E Low-Dimens Syst Nanostruct 44:440–447. https://doi.org/10.1016/j.physe.2011.09.018
Shaabani A, Tavasoli-Rad F, Lee D (2005) Potassium permanganate oxidation of organic compounds. Synth Commun 35:571–580. https://doi.org/10.1081/SCC-200049792
Shen B, Linko V, Tapio K, Pikker S, Lemma T, Gopinath A, Gothelf K, Kostiainen M, Toppari J (2018) Plasmonic nanostructures through DNA-assisted lithography. Sci Adv 4:1–7. https://doi.org/10.1126/sciadv.aap8978
Singh A, Kaur K (2019) Biological and physical applications of silver nanoparticles with emerging trends of green synthesis. In: Silver Nanoparticles-Health and Safety. IntechOpen, pp 1–25. https://doi.org/10.5772/intechopen.88684
Sivanesan K, Jayakrishnan P, Razack SA, Sellaperumal P, Ramakrishnan G, Sahadevan R (2017) Biofabrication of manganese nanoparticle using Aegle marmelos fruit extract and assessment of its biological actvities. Nanomed Res J 2:171–178. https://doi.org/10.22034/nmrj.2017.03.005
Some S, Bulut O, Biswas K, Kumar A, Roy A, Sen K, Mandal A, Franco O, Ince I, Neog K, Das S, Pradhan S, Dutta S, Bhattacharjya D, Saha S, Mohapatra P, Bhuimali A, Unni B, Kati A, Mandal A, Yilmaz M, Ocsoy I (2019) Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae). Sci Rep 9:1–13. https://doi.org/10.1038/s41598-019-50906-6
Tetyana P, Prozesky EA, Jäger AK, Meyer JJM, van Staden J (2002) Some medicinal properties of Cussonia and Schefflera species used in traditional medicine. South Afr J Bot 68:51–54. https://doi.org/10.1016/S0254-6299(16)30454-9
Wang H, Lu Z, Qian D, Li Y, Zhang W (2007) Single-crystal α-MnO2 nanorods: synthesis and electrochemical properties. Nanotechnology 18:115616. https://doi.org/10.1088/0957-4484/18/11/115616
Wang M, Zhang L, Huang W, Zhou Y, Zhao H, Lv J, Tian J, Kan X, Shi J (2017) Pt/MnO2 nanosheets: facile synthesis and highly efficient catalyst for ethylene oxidation at low temperature. RSC Adv 7:14809–14815. https://doi.org/10.1039/c6ra26529d
Zhai T, Wang F, Yu M, Xie M, Liang C, Li C, Xiao F, Tang R, Wu Q, Lu X, Tong Y (2013) 3D MnO2–graphene composites with large areal capacitance for high-performance asymmetric supercapacitors. Nanoscale 5:6790–6796. https://doi.org/10.1039/c3nr01589k
Zheng R, Xu X, Tian Z, Yang J (2009) Chemical constituents from the fruits of Hippophae rhamnoides. Nat Prod Res 23:1451–1456. https://doi.org/10.1080/14786410903075457
Acknowledgements
The authors acknowledge the Microscopy and Microanalysis Unit, Instrumental Laboratory, NMR Unit, and UKZN Nanotechnology Platform, UKZN. This research was funded, in part, by the National Research Foundation through Dr Roshila Moodley (14008) and Dr Nomfundo T Mahlangeni (106490).
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Mahlangeni, N.T., Magura, J., Moodley, R. et al. Biogenic synthesis, antioxidant and antimicrobial activity of silver and manganese dioxide nanoparticles using Cussonia zuluensis Strey. Chem. Pap. 74, 4253–4265 (2020). https://doi.org/10.1007/s11696-020-01244-9
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DOI: https://doi.org/10.1007/s11696-020-01244-9