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Sleeve Gastrectomy and Roux-en-Y Gastric Bypass Attenuate Pro-inflammatory Small Intestinal Cytokine Signatures

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Abstract

Background

Bariatric surgery rapidly induces improvements in type 2 diabetes (T2D) in concert with reduction in systemic markers of inflammation. The impact of bariatric surgery on local intestinal immunity is not known. We hypothesize that sleeve gastrectomy (SG) and gastric bypass (RYGB) surgeries resolve obesity-induced intestinal inflammation, thereby promoting T2D resolution.

Methods

SG and RYGB, or control surgery was performed in SD rats (n = 4–6/group). Key cytokines involved in insulin resistance (TNF-α, IFN-γ), inflammasome activation (IL-1β, IL-18), inflammation resolution (IL-10, IL-33), and Th17 cell responses (IL-17, IL-23) were measured by qPCR in mucosal scrapings of jejunum at 4 weeks post-surgery. Intestinal cytokine expressions were correlated with weight change, systemic and portal glucose, and insulin levels in response to an enteral glucose load.

Results

SG downregulated IL-17 and IL-23 in both proximal and distal jejunum, and IFN-γ was reduced only in distal jejunum (p < 0.05). Jejunal IL-17 and IL-23 expression correlated positively with weight changes after SG (0.93 and 0.98, respectively; p < 0.05). Changes in IFN-γ correlated strongly with insulin levels in portal and systemic circulation (0.99 and 0.95, respectively, p < 0.05). As with SG, IFN-γ, IL-17, and IL-23 were significantly reduced by RYGB. RYGB also reduced TNF-α and IL-18 and increased IL-33 levels (p < 0.05).

Conclusions

RYGB and SG reduce expression of pro-inflammatory cytokines IL-17, IL-23, and IFN-γ in the jejunum. RYGB showed attenuation of additional pro-inflammatory cytokines and enhanced expression of IL-33. Post-surgical changes in intestinal IL-17, IL-23, and IFN-γ correlate strongly with changes in weight and glucose-triggered insulin responses.

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Acknowledgments

This study was supported by a Pilot and Feasibility Grant from the Boston Area Diabetes Endocrinology Research Center (P30 DK057521) and a KL2 Award from Harvard Catalyst (4KL2TR001100-04).

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Authors and Affiliations

  1. Department of Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, 02115, USA

    Renuka Subramaniam, Hassan Aliakbarian, Hina Y. Bhutta, David A. Harris, Ali Tavakkoli & Eric G. Sheu

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  1. Renuka Subramaniam
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  2. Hassan Aliakbarian
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  3. Hina Y. Bhutta
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  4. David A. Harris
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  5. Ali Tavakkoli
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  6. Eric G. Sheu
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Correspondence to Eric G. Sheu.

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Subramaniam, R., Aliakbarian, H., Bhutta, H.Y. et al. Sleeve Gastrectomy and Roux-en-Y Gastric Bypass Attenuate Pro-inflammatory Small Intestinal Cytokine Signatures. OBES SURG 29, 3824–3832 (2019). https://doi.org/10.1007/s11695-019-04059-0

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