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Duodenal-Jejunal Bypass Maintains Gut Permeability by Suppressing Gut Inflammation

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Abstract

Background

The aim of this study was to investigate gut inflammation and permeability in rats after duodenal-jejunal bypass (DJB) and in rats injected with a glucagon-like peptide-1 (GLP-1) receptor analog.

Methods

Twelve male 16-week-old obese diabetic rats were divided into three groups: the DJB group, the sham group, and the group injected daily with a GLP-1 receptor agonist (liraglutide). Gut inflammation and the expression of tight junction protein (claudin-1) were analyzed in the three groups at 8 weeks after surgery.

Results

The DJB group showed significantly lower levels of gut inflammatory cytokines than the liraglutide group. Claudin-1 showed stronger intensity on immunofluorescent staining in the DJB group than that in the liraglutide group.

Conclusions

In summary, DJB surgery might maintain gut permeability via suppression of gut inflammation.

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Abbreviations

DJB:

Duodenal-jejunal bypass

GLP-1:

Glucagon-like peptide-1

HFD:

High-fat diet

RNA:

Ribonucleic acid

RT:

Reverse transcription

PBS:

Phosphate-buffered salts

DAPI:

4′,6-Diamidino-2-phenylindole

LPS:

Lipopolysaccharide

NASH:

Nonalcoholic steatohepatitis

References

  1. Goldfine AB, Shoelson SE, Aguirre V. Expansion and contraction: treating diabetes with bariatric surgery. Nat Med. 2009;15(6):616–7.

    Article  CAS  PubMed  Google Scholar 

  2. Ohta M, Seki Y, Wong SK, et al. Bariatric/metabolic surgery in the Asia-Pacific region: APMBSS 2018 survey. Obes Surg. 2019;29(2):534–41.

    Article  PubMed  Google Scholar 

  3. Rubino F, Forgione A, Cummings DE, et al. The mechanism of diabetes control after gastrointestinal bypass surgery reveals a role of the proximal small intestine in the pathophysiology of type 2 diabetes. Ann Surg. 2006;244:741–9.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Kashihara H, Shimada M, Kurita N, et al. Duodenal-jejunal bypass improves diabetes and liver steatosis via enhanced glucagon-like peptide-1 elicited by bile acids. J Gastroenterol Hepatol. 2015;30(2):308–15.

    Article  CAS  PubMed  Google Scholar 

  5. Kashihara H, Shimada M, Yoshikawa K, et al. Duodenal-jejunal bypass changes the composition of the gut microbiota. Surg Today. 2017;47(1):137–40.

    Article  CAS  PubMed  Google Scholar 

  6. Gulhane M, Murray L, Lourie R, et al. High fat diets induce colonic epithelial cell stress and inflammation that is reversed by IL-22. Sci Rep. 2016;6:28990.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Borst SE. The role of TNF-alpha in insulin resistance. Endocrine. 2004;23(2–3):177–82.

    Article  CAS  PubMed  Google Scholar 

  8. Wellen KE, Hotamisligil GS. Inflammation, stress, and diabetes. J Clin Invest. 2005;115(5):1111–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Jager J, Grémeaux T, Cormont M, et al. Interleukin-1beta-induced insulin resistance in adipocytes through down-regulation of insulin receptor substrate-1 expression. Endocrinology. 2007;148(1):241–51.

    Article  CAS  PubMed  Google Scholar 

  10. Šestan M, Marinović S, Kavazović I, et al. Virus-induced interferon-γ causes insulin resistance in skeletal muscle and derails glycemic control in obesity. Immunity. 2018;49(1):164–77.

    Article  PubMed  CAS  Google Scholar 

  11. Kawano Y, Nakae J, Watanabe N, et al. Colonic pro-inflammatory macrophages cause insulin resistance in an intestinal Ccl2/Ccr2-dependent manner. Cell Metab. 2016;24(2):295–310.

    Article  CAS  PubMed  Google Scholar 

  12. Guo Y, Liu CQ, Liu GP, et al. Roux-en-Y gastric bypass decreases endotoxemia and inflammatory stress in association with improvements in gut permeability in obese diabetic rats. J Diabetes. 2019; https://doi.org/10.1111/1753-0407.12906.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Surgery, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8503, Japan

    Hideya Kashihara, Mitsuo Shimada, Kozo Yoshikawa, Jun Higashijima, Tomohiko Miyatani, Takuya Tokunaga, Masaaki Nishi & Chie Takasu

Authors
  1. Hideya Kashihara
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  2. Mitsuo Shimada
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  3. Kozo Yoshikawa
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  4. Jun Higashijima
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  5. Tomohiko Miyatani
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  6. Takuya Tokunaga
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  7. Masaaki Nishi
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  8. Chie Takasu
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Corresponding author

Correspondence to Hideya Kashihara.

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All applicable institutional and/or national guidelines for the care and use of animals were followed. Informed consent statement does not apply to this study.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Kashihara, H., Shimada, M., Yoshikawa, K. et al. Duodenal-Jejunal Bypass Maintains Gut Permeability by Suppressing Gut Inflammation. OBES SURG 29, 2745–2749 (2019). https://doi.org/10.1007/s11695-019-03922-4

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  • DOI: https://doi.org/10.1007/s11695-019-03922-4

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