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Duodenal-jejunal bypass changes the composition of the gut microbiota

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Abstract

This study investigated the composition of the gut microbiota in rats after duodenal-jejunal bypass (DJB) in comparison to that in rats injected with a glucagon-like peptide-1 (GLP-1) receptor analog. Six male 16-week-old OLETF rats were divided into three groups: a DJB group, a sham operation group, and a daily injection with a GLP-1 receptor agonist (liraglutide) group. The gut microbiota of the three groups was analyzed at postoperative week 8 using the PCR-clone library method targeting the 16S ribosomal RNA gene. The DJB group showed a decrease in Bacteroidia in comparison to the other two groups (DJB, 3.9 %/2.8 %; sham, 10.8 %/11.6 %; liraglutide, 33.2 %/14.1 %). In addition, DJB markedly increased the ratio of Gammaproteobacteria (DJB, 10.8 %/13.7 %; sham, 0.2 %/1.2 %; liraglutide, 0 %/0.1 %). DJB changes the composition of gut microbiota; these changes might be the factors that contribute to the effects of DJB.

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Fig. 1

Abbreviations

DJB:

Duodenal-jejunal bypass

GLP-1:

Glucagon-like peptide-1

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Acknowledgments

We are thankful to the staff of the Department of Surgery, Institute of Health Biosciences, University of Tokushima, for their contributions.

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Authors and Affiliations

  1. Department of Surgery, Institute of Health Biosciences, The University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8503, Japan

    Hideya Kashihara, Mitsuo Shimada, Kozo Yoshikawa, Jun Higashijima, Toshihiro Nakao, Masaaki Nishi & Chie Takasu

Authors
  1. Hideya Kashihara
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  2. Mitsuo Shimada
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  3. Kozo Yoshikawa
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  4. Jun Higashijima
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  5. Toshihiro Nakao
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  6. Masaaki Nishi
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  7. Chie Takasu
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Corresponding author

Correspondence to Hideya Kashihara.

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The authors declare no conflicts of interest in association with the present study.

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Kashihara, H., Shimada, M., Yoshikawa, K. et al. Duodenal-jejunal bypass changes the composition of the gut microbiota. Surg Today 47, 137–140 (2017). https://doi.org/10.1007/s00595-016-1373-x

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