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Circulating ANGPTL8/Betatrophin Concentrations Are Increased After Surgically Induced Weight Loss, but Not After Diet-Induced Weight Loss

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Abstract

Background

ANGPTL8/betatrophin is a secreted protein reported to be involved in β-cell replication that has recently been shown to be more related to lipid metabolism. Weight loss represents a clinical situation of improvement of glucose homeostasis and overall metabolic control. The aim of the present study was to analyze the impact of weight loss induced by either a conventional dietary treatment or bariatric surgery on ANGPTL8/betatrophin concentrations.

Methods

Serum concentrations of ANGPTL8/betatrophin were measured by ELISA in 158 subjects before and 1 year after weight loss induced either by conventional dietary treatment (n = 38) or bariatric surgery (sleeve gastrectomy, n = 20, or Roux-en-Y gastric bypass, n = 100).

Results

Massive surgery-induced weight loss after SG or RYGB was accompanied by a statistically significant increase in circulating levels of ANGPTL8/betatrophin (28.1 ± 13.9 to 40.3 ± 22.8 ng/mL, P = 0.001 after SG; 24.6 ± 10.9 to 41.7 ± 19.4 ng/mL, P < 0.001 after RYGB), while remaining unchanged 25.6 ± 13.3 to 25.4 ± 10.7 ng/mL (P = 0.891) after diet-induced weight loss. The change in ANGPTL8/betatrophin levels was positively correlated with the change in HDL-C concentrations.

Conclusions

Our study showed that serum ANGPTL8/betatrophin concentrations were increased in obese subjects after surgically induced weight loss, but not after weight loss achieved by conventional dietary treatment. The change in ANGPTL8/betatrophin concentrations emerged as a significant predictor of the change in HDL-C levels after weight loss.

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Abbreviations

ANGPTL8:

Angiopoietin-like protein 8

BMI:

Body mass index

SBP:

Systolic blood pressure

DBP:

Diastolic blood pressure

OGTT:

Oral glucose tolerance test

HOMA:

Homeostatic model assessment

PAL:

Physical activity level

QUICKI:

Quantitative insulin sensitivity check index

CRP:

C-reactive protein

ALT:

Alanine aminotransferase

γ-GT:

γ-Glutamyltransferase

WBC:

White blood cells

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Acknowledgments

The authors gratefully acknowledge the valuable collaboration of all the members of the Nutrition Unit for their technical support. The authors also wish to thank all subjects who participated in this study.

This work was supported by grants from the Fondo de Investigación Sanitaria-FEDER, Instituto de Salud Carlos III (ISCIII) (PI12/00515, PI13/00460 and PI/14/00950) and the ISCIII, Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y Nutrición, CIBEROBN, Spain. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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    Authors and Affiliations

    1. Department of Endocrinology and Nutrition, Clínica Universidad de Navarra, Avda. Pío XII 36, 31008, Pamplona, Spain

      Eider Pascual-Corrales, Camilo Silva, Javier Salvador & Gema Frühbeck

    2. Metabolic Research Laboratory, University of Navarra, Pamplona, Spain

      Javier Gómez-Ambrosi, Victoria Catalán, Amaia Rodríguez, Beatriz Ramírez & Gema Frühbeck

    3. CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Pamplona, Spain

      Javier Gómez-Ambrosi, Rafael Moncada, Víctor Valentí, Victoria Catalán, Amaia Rodríguez, Beatriz Ramírez, Camilo Silva, María Jesús Gil, Javier Salvador & Gema Frühbeck

    4. Obesity & Adipobiology Group, Instituto de Investigaciones Sanitarias de Navarra (IdiSNA), Pamplona, Spain

      Javier Gómez-Ambrosi, Rafael Moncada, Víctor Valentí, Victoria Catalán, Amaia Rodríguez, Beatriz Ramírez, Camilo Silva, María Jesús Gil, Javier Salvador & Gema Frühbeck

    5. Department of Anesthesia, Clínica Universidad de Navarra, Pamplona, Spain

      Rafael Moncada

    6. Department of Surgery, Clínica Universidad de Navarra, Pamplona, Spain

      Víctor Valentí

    7. Department of Biochemistry, Clínica Universidad de Navarra, Pamplona, Spain

      María Jesús Gil

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    1. Eider Pascual-Corrales
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    Correspondence to Gema Frühbeck.

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    Conflict of Interest

    E.P.-C., J.G.-A., R.M., V.V., V.C., A.R., B.R., C.S., M.J.G, J.S., and G.F. declare that they have no conflict of interest.

    Additional information

    E. Pascual-Corrales and J. Gómez-Ambrosi contributed equally to this work.

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    Effect of weight loss on ANGPTL8/betatrophin concentrations in obese patients matched by body adiposity change and gender before (Pre) and after (Post) a conventional dietary (Conv Diet) or surgical [sleeve gastrectomy (SG) or Roux-en-Y gastric bypass (RYGB)] intervention. Box represents interquartile range and median inside, with whiskers showing 10/90 percentiles. Differences between pre and post weight loss were analyzed by paired two-tailed Student’s t tests. *P<0.05 and **P<0.01 vs pre weight loss values (PDF 18 kb)

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    Pascual-Corrales, E., Gómez-Ambrosi, J., Moncada, R. et al. Circulating ANGPTL8/Betatrophin Concentrations Are Increased After Surgically Induced Weight Loss, but Not After Diet-Induced Weight Loss. OBES SURG 26, 1881–1889 (2016). https://doi.org/10.1007/s11695-015-2026-7

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