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Gene Expression Patterns in Hepatic Tissue and Visceral Adipose Tissue of Patients with Non-Alcoholic Fatty Liver Disease

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Background

Non-alcoholic fatty liver disease (NAFLD) and its progressive form, non-alcoholic steatohepatitis (NASH), are among the least understood metabolic consequences of obesity. Increasingly, omental adipose tissue is recognized as a biologically active organ in the pathogenesis of NAFLD. Differences in transcriptional regulation in omental adipose tissue and liver tissue may provide important insights into the pathogenesis of NAFLD and its progression.

Methods

Transcriptional profiles were obtained for liver and visceral adipose specimens of morbidly obese patients undergoing bariatric surgery. Functional analyses with the Ingenuity Pathways Knowledge Base (IPKB) and IPA 4.0 software identified genes that potentially play hepatoprotective roles as well as those potentially involved in the pathogenesis of NASH.TNF〈 and IL6 were measured in the serum samples.

Results

Tissue from patients with NASH showed prominent adipose-specific deregulation of genes related to inflammation and the immune system. A number of liver and adipose-specific functional networks, including those centered at TNF〈, JUN/JUNB, and IFN© were highlighted as related to the NASH pathogenesis.The results also showed compensatory increases in hepatic detoxification enzymes and decreases in the gene network controlled by transcription factor COUP-TFII.

Conclusion

Our findings support the hypothesis that adipocyte secretion plays an important role in the development of NAFLD.

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Authors and Affiliations

  1. Center for Liver Diseases, Inova Fairfax Hospital, Fairfax, VA, USA

    Ancha Baranova, Karen Schlauch, Hazem Elariny, Chase Bennett, Clare Nugent, Zahra Younoszai, Rochelle Collantes & Zobair M. Younossi

  2. Center for the Study of Genomics in Liver Diseases, Molecular and Microbiology Department, George Mason University, Fairfax, VA, USA

    Ancha Baranova, Mohammed Jarrar, Shobha J. Gowder, Rochelle Collantes, Vikas Chandhoke & Zobair M. Younossi

  3. Department of Genetics and Genomics, Boston University School of Medicine, Boston, MA, USA

    Karen Schlauch

  4. Center for Liver Diseases, Inova Fairfax Hospital, 3300 Gallows Road, Falls Church, VA, 22042, USA

    Zobair M. Younossi

Authors
  1. Ancha Baranova
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  2. Karen Schlauch
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  3. Hazem Elariny
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  4. Mohammed Jarrar
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  5. Chase Bennett
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  6. Clare Nugent
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  7. Shobha J. Gowder
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  8. Zahra Younoszai
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  9. Rochelle Collantes
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  10. Vikas Chandhoke
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  11. Zobair M. Younossi
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Corresponding author

Correspondence to Zobair M. Younossi.

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Baranova, A., Schlauch, K., Elariny, H. et al. Gene Expression Patterns in Hepatic Tissue and Visceral Adipose Tissue of Patients with Non-Alcoholic Fatty Liver Disease. OBES SURG 17, 1111–1118 (2007). https://doi.org/10.1007/s11695-007-9187-y

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  • DOI: https://doi.org/10.1007/s11695-007-9187-y

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