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Probiotic viability, physicochemical, and sensory properties of probiotic orange juice

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Abstract

Probiotics can be incorporated into fruit juices as an alternative to milk products. In this study, Lactobacillus acidophilus B103 was used as a probiotic ingredient in an orange juice model at concentration levels of 0, 1, and 2%. The juice was stored at 4 and 25 °C for 21 days, and evaluated for physicochemical stability (pH, titratable acidity, and color), probiotic survival, and sensory acceptance. The probiotic-loaded orange juices stored at 4 °C showed no undesirable alteration in pH and acidity during storage. The juice with 2% L. acidophilus B103 stored at 4 °C had the lowest color changes compared to the other samples, and it also contained the highest count of viable cells at the ends of storage time (~ 7.92 log CFU/ml). The highest sensory acceptance (i.e., color, aroma, and overall acceptance) was also found in L. acidophilus B103-loaded orange juices during storage at 4 °C. Consequently, orange juice could serve as a suitable carrier for L. acidophilus B103.

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References

  1. J.C. Amorim, R.H. Piccoli, W.F. Duarte, Probiotic potential of yeasts isolated from pineapple and their use in the elaboration of potentially functional fermented beverages. Food Res. Int. 107, 518–527 (2018). https://doi.org/10.1016/j.foodres.2018.02.054

    Article  CAS  PubMed  Google Scholar 

  2. H. Barzegar, B. Alizadeh Behbahani, F. Falah, Safety, probiotic properties, antimicrobial activity, and technological performance of Lactobacillus strains isolated from Iranian raw milk cheeses. Food Sci. Nutr. 9(8), 4094–4107 (2021). https://doi.org/10.1002/fsn3.2365

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. W.H. Holzapfel, P. Haberer, J. Snel, U. Schillinger, J.H.J. Huis in’t Veld, Overview of gut flora and probiotics. Int. J. Food Microbiol. 41(2), 85–101 (1998). https://doi.org/10.1016/S0168-1605(98)00044-0

    Article  CAS  PubMed  Google Scholar 

  4. M. Perricone, A. Bevilacqua, C. Altieri, M. Sinigaglia, M.R. Corbo, Challenges for the production of probiotic fruit juices. Beverages (2015). https://doi.org/10.3390/beverages1020095

    Article  Google Scholar 

  5. A. Vasiee, F. Falah, B. Alizadeh Behbahani, F. Tabatabaee-yazdi, Probiotic characterization of Pediococcus strains isolated from Iranian cereal-dairy fermented product: interaction with pathogenic bacteria and the enteric cell line Caco-2. J. Biosci. Bioeng. 130(5), 471–479 (2020). https://doi.org/10.1016/j.jbiosc.2020.07.002

    Article  CAS  PubMed  Google Scholar 

  6. M. Saboktakin-Rizi, B. Alizadeh Behbahani, M. Hojjati, M. Noshad, Identification of Lactobacillus plantarum TW29-1 isolated from Iranian fermented cereal-dairy product (Yellow Zabol Kashk): probiotic characteristics, antimicrobial activity and safety evaluation. J. Food Meas. Charact. 15(3), 2615–2624 (2021). https://doi.org/10.1007/s11694-021-00846-5

    Article  Google Scholar 

  7. A. Vasiee, B. Alizadeh Behbahani, F. Tabatabaei Yazdi, S.A. Mortazavi, H. Noorbakhsh, Diversity and probiotic potential of lactic acid bacteria isolated from horreh, a traditional Iranian fermented food. Probiotics Antimicrob Proteins 10(2), 258–268 (2018). https://doi.org/10.1007/s12602-017-9282-x

    Article  CAS  PubMed  Google Scholar 

  8. M.H. Marhamatizadeh, S. Sayyadi, Mining of lactic acid bacteria from traditional yogurt (Mast) of Iran for possible industrial probiotic use. Italian J. Anim. Sci. 18(1), 663–667 (2019). https://doi.org/10.1080/1828051X.2018.1552541

    Article  CAS  Google Scholar 

  9. M.K. Sharifi Yazdi, A. Davoodabadi, H.R. Khesht Zarin, M. Tajabadi Ebrahimi, M.M. Soltan Dallal, Characterisation and probiotic potential of lactic acid bacteria isolated from Iranian traditional yogurts. Italian J. Anim. Sci. 16(2), 185–188 (2017). https://doi.org/10.1080/1828051X.2016.1222888

    Article  Google Scholar 

  10. B. Kos, J. Šušković, S. Vuković, M. Šimpraga, J. Frece, S. Matošić, Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J. Appl. Microbiol. 94(6), 981–987 (2003). https://doi.org/10.1046/j.1365-2672.2003.01915.x

    Article  CAS  PubMed  Google Scholar 

  11. B.T. Nguyen, E. Bujna, N. Fekete, A.T.M. Tran, J.M. Rezessy-Szabo, R. Prasad, Q.D. Nguyen, Probiotic beverage from pineapple juice fermented with Lactobacillus and Bifidobacterium strains. Front. Nutr. (2019). https://doi.org/10.3389/fnut.2019.00054

    Article  PubMed  PubMed Central  Google Scholar 

  12. M.G.M. Costa, T.V. Fonteles, A.L.T. de Jesus, S. Rodrigues, Sonicated pineapple juice as substrate for L. casei cultivation for probiotic beverage development: process optimisation and product stability. Food Chem. 139(1), 261–266 (2013). https://doi.org/10.1016/j.foodchem.2013.01.059

    Article  CAS  PubMed  Google Scholar 

  13. A.L. Fernandes Pereira, S. Rodrigues, Chapter 15 - Turning fruit juice into probiotic beverages, in Fruit Juices. ed. by G. Rajauria, B.K. Tiwari (Academic Press, San Diego, 2018), pp.279–287. https://doi.org/10.1016/B978-0-12-802230-6.00015-1

    Chapter  Google Scholar 

  14. Y. Lu, C.-W. Tan, D. Chen, S.-Q. Liu, Potential of three probiotic lactobacilli in transforming star fruit juice into functional beverages. Food Sci. Nutr. 6(8), 2141–2150 (2018). https://doi.org/10.1002/fsn3.775

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. A. Sohail, M.S. Turner, E.K. Prabawati, A.G.A. Coombes, B. Bhandari, Evaluation of Lactobacillus rhamnosus GG and Lactobacillus acidophilus NCFM encapsulated using a novel impinging aerosol method in fruit food products. Int. J. Food Microbiol. 157(2), 162–166 (2012). https://doi.org/10.1016/j.ijfoodmicro.2012.04.025

    Article  CAS  PubMed  Google Scholar 

  16. W. Ding, N.P. Shah, Survival of free and microencapsulated probiotic bacteria in orange and apple juices. Int. Food Res. J. 15(2), 219–232 (2008)

    Google Scholar 

  17. B. Alizadeh Behbahani, M. Noshad, F. Falah, Inhibition of Escherichia coli adhesion to human intestinal Caco-2 cells by probiotic candidate Lactobacillus plantarum strain L15. Microb. Pathog. 136, 103677 (2019). https://doi.org/10.1016/j.micpath.2019.103677

    Article  CAS  PubMed  Google Scholar 

  18. M. Hojjati, B. Alizadeh Behbahani, F. Falah, Aggregation, adherence, anti-adhesion and antagonistic activity properties relating to surface charge of probiotic Lactobacillus brevis gp104 against Staphylococcus aureus. Microb. Pathog. 147, 104420 (2020). https://doi.org/10.1016/j.micpath.2020.104420

    Article  CAS  PubMed  Google Scholar 

  19. A.L.F. Pereira, T.C. Maciel, S. Rodrigues, Probiotic beverage from cashew apple juice fermented with Lactobacillus casei. Food Res. Int. 44(5), 1276–1283 (2011). https://doi.org/10.1016/j.foodres.2010.11.035

    Article  CAS  Google Scholar 

  20. M.R.A. Porto, V.S. Okina, T.C. Pimentel, S. Garcia, S.H. Prudencio, Beet and orange mixed juices added with. Nutr. Food Sci. 48(1), 76–87 (2018). https://doi.org/10.1108/NFS-06-2017-0108

    Article  Google Scholar 

  21. L.C.R. dos Reis, E.M.P. Facco, S.H. Flôres, A.O. Rios, Stability of functional compounds and antioxidant activity of fresh and pasteurized orange passion fruit (Passiflora caerulea) during cold storage. Food Res. Int. 106, 481–486 (2018). https://doi.org/10.1016/j.foodres.2018.01.019

    Article  CAS  PubMed  Google Scholar 

  22. G.M. da Costa, J.V. de Carvalho Silva, J.D. Mingotti, C.E. Barão, S.J. Klososki, T.C. Pimentel, Effect of ascorbic acid or oligofructose supplementation on L. paracasei viability, physicochemical characteristics and acceptance of probiotic orange juice. LWT 75, 195–201 (2017). https://doi.org/10.1016/j.lwt.2016.08.051

    Article  CAS  Google Scholar 

  23. O.N. Donkor, A. Henriksson, T. Vasiljevic, N.P. Shah, Effect of acidification on the activity of probiotics in yoghurt during cold storage. Inter. Dairy J. 16(10), 1181–1189 (2006). https://doi.org/10.1016/j.idairyj.2005.10.008

    Article  CAS  Google Scholar 

  24. S.M.B. Hashemi, D. Jafarpour, M. Jouki, Improving bioactive properties of peach juice using Lactobacillus strains fermentation: antagonistic and anti-adhesion effects, anti-inflammatory and antioxidant properties, and Maillard reaction inhibition. Food Chem. 365, 130501 (2021). https://doi.org/10.1016/j.foodchem.2021.130501

    Article  CAS  PubMed  Google Scholar 

  25. S. Nualkaekul, I. Salmeron, D. Charalampopoulos, Investigation of the factors influencing the survival of Bifidobacterium longum in model acidic solutions and fruit juices. Food Chem. 129(3), 1037–1044 (2011). https://doi.org/10.1016/j.foodchem.2011.05.071

    Article  CAS  PubMed  Google Scholar 

  26. H.S. Lee, G.A. Coates, Effect of thermal pasteurization on Valencia orange juice color and pigments. LWT 36(1), 153–156 (2003). https://doi.org/10.1016/S0023-6438(02)00087-7

    Article  CAS  Google Scholar 

  27. T.C. Pimentel, G.S. Madrona, S. Garcia, S.H. Prudencio, Probiotic viability, physicochemical characteristics and acceptability during refrigerated storage of clarified apple juice supplemented with Lactobacillus paracasei ssp. paracasei and oligofructose in different package type. LWT 63(1), 415–422 (2015). https://doi.org/10.1016/j.lwt.2015.03.009

    Article  CAS  Google Scholar 

  28. Y. Rivera-Espinoza, Y. Gallardo-Navarro, Non-dairy probiotic products. Food Microbiol. 27(1), 1–11 (2010). https://doi.org/10.1016/j.fm.2008.06.008

    Article  PubMed  Google Scholar 

  29. S. Nualkaekul, D. Charalampopoulos, Survival of Lactobacillus plantarum in model solutions and fruit juices. Int. J. Food Microbiol. 146(2), 111–117 (2011). https://doi.org/10.1016/j.ijfoodmicro.2011.01.040

    Article  CAS  PubMed  Google Scholar 

  30. M.E. Parish, G.D. Sadler, L. Wicker, Viability of Lactobacillus plantarum in orange juice under low pH and temperature conditions. J. Food Sci. 55(4), 1023–1025 (1990). https://doi.org/10.1111/j.1365-2621.1990.tb01588.x

    Article  Google Scholar 

  31. T.C. Pimentel, G.S. Madrona, S.H. Prudencio, Probiotic clarified apple juice with oligofructose or sucralose as sugar substitutes: sensory profile and acceptability. LWT 62(1, Part 2), 838–846 (2015). https://doi.org/10.1016/j.lwt.2014.08.001

    Article  CAS  Google Scholar 

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Acknowledgements

The authors would like to express their sincere gratitude to the Vice-chancellor for Research and Technology of Agricultural Sciences and Natural Resources University of Khuzestan for supporting this study.

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Authors and Affiliations

  1. Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, 6341773637, Iran

    Zahra Kardooni, Behrooz Alizadeh Behbahani, Hossein Jooyandeh & Mohammad Noshad

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  1. Zahra Kardooni
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  2. Behrooz Alizadeh Behbahani
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  3. Hossein Jooyandeh
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  4. Mohammad Noshad
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Contributions

ZK: conceptualization, investigation, writing—original draft; BAB: conceptualization, resources, supervision, methodology, writing: review & editing; HJ: conceptualization, resources, supervision, methodology, writing: review & editing; MN: investigation, methodology, writing—review & editing.

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Correspondence to Behrooz Alizadeh Behbahani.

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Kardooni, Z., Alizadeh Behbahani, B., Jooyandeh, H. et al. Probiotic viability, physicochemical, and sensory properties of probiotic orange juice. Food Measure 17, 1817–1822 (2023). https://doi.org/10.1007/s11694-022-01771-x

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  • DOI: https://doi.org/10.1007/s11694-022-01771-x

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