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Physicochemical and antibacterial effect of Soy Protein Isolate/Gelatin electrospun nanofibres incorporated with Zataria multiflora and Cinnamon zeylanicum essential oils

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Abstract

Recently, combination of nanotechnology and active food packaging provided new horizon to extend the shelf life of food. This study aimed to fabricate natural nanofibrous film incorporated with essential oils using the nanospinning technique and examine its antimicrobial activity. Constituents of Zataria multiflora and Cinnamon zeylanicum essential oils (EOs) were investigated by Gas Chromatography–Mass Spectrometry and their antimicrobial properties were examined. Subsequently, electrospun Soy Protein Isolate (SPI)/Gelatin was embedded with Zataria multiflora and Cinnamon zeylanicum EOs. Scanning Electron Microscope (SEM), Fourier Transform Infrared Spectrometry (FTIR), and Thermogravimetric analysis (TGA) were done to evaluate the properties of electrospun fibers. The antimicrobial activity of nanofibrous films was evaluated. SEM revealed that the addition of 30% gelatin neutralized SPI charge, representing a significant increase in the diameter of nanofibers by 314.08 nm. Furthermore, TGA showed stability for Soy/Gel nanofibers. The fabricated nanofibrous film exhibited exquisite antimicrobial activity against Gram-negative and Gram-positive pathogens. For example, SPI-gelatin nanofibrous film incorporated with 20% Z. multiflora reduced 100% of S. aureus, B. cereus, and L.monocytogenes. The reduction for E. coli and S. typhimurium were 70% and 63%, respectively. We suggest that incorporating EOs with nanofibrous films can be a promising method to extend the shelf life of food and can be used in production for active food packaging.

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Acknowledgements

The authors would like to appreciate National Institute for Medical Research Development (NIMAD) for financially supporting this study (Project Number 964459).

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Authors and Affiliations

  1. Food, Drug and Natural Products Health Research Center, Golestan University of Medical Sciences, Gorgan, Iran

    Mojtaba Raeisi

  2. Student Research Committee, Department of Food Technology, Faculty of Nutrition Science and Food Technology, Nutritional and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Masoud Aman Mohammadi

  3. Department of Fish Processing and Technologies, Faculty of Fisheries, Firat University, Elazig, Turkey

    Ozlem Emir Coban

  4. Trita Nanomedicine Research Center (TNRC), Trita Third Millennium Pharmaceuticals, 4533155681, Zanjan, Iran

    Soghra Ramezani

  5. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Marjan Ghorbani

  6. Nutrition Research Center and Department of Food Science and Technology, Faculty of Nutrition and Food Science, Tabriz University of Medical Sciences, Tabriz, Iran

    Mahnaz Tabibiazar

  7. Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

    Rahem khoshbakht

  8. Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Seyyed Mohammad Ali Noori

  9. Department of Nutrition, School of Allied Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Seyyed Mohammad Ali Noori

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  1. Mojtaba Raeisi
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Raeisi, M., Mohammadi, M.A., Coban, O.E. et al. Physicochemical and antibacterial effect of Soy Protein Isolate/Gelatin electrospun nanofibres incorporated with Zataria multiflora and Cinnamon zeylanicum essential oils. Food Measure 15, 1116–1126 (2021). https://doi.org/10.1007/s11694-020-00700-0

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