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Phytochemical evaluation, antioxidant properties and antibacterial activity of Iranian medicinal herb Galanthus transcaucasicus Fomin

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Abstract

This study was performed to determine bioactive compounds such as flavonoid and phenolic contents by HPLC and gas chromatography–mass spectrophotometry (GC–MS) and evaluate their antioxidant and antibacterial activities in bulb, flower and shoot extracts of Galanthus transcaucasicus Fomin. The overall results indicated that shoot part contained higher amounts of phenolics and flavonoids compared to the bulb and flower with respective values of 4.45and 2.67 mg/g DW. Besides, HPLC analyses showed that the gallic acid and syringic acid were the major phenolic contents in bulb, flower and shoot of G. transcaucasicus extracts. Apart from that, naringin was the main flavonoid compounds in bulb, flower and shoot. Volatile metabolites were detected by GC–MS screening that indicated 2-furancarboxaldehyde, 2,3-butanediol, and acetic acid as the main compounds in the bulb, flower and shoot extracts. The results of antioxidant activity test demonstrated that shoot extracts of G. transcaucasicus exhibited higher antioxidant activities compare to bulb and flower. Furthermore, the shoot, flower and bulb extracts showed high, moderate and weak antibacterial activities against common human and food borne pathogenic bacteria, respectively. The overall results revealed that various phytochemicals and bioactive compound in different parts of G. transcaucasicus could be considered as a source of putative antioxidant and antibacterial properties that could be used as biopreservative in food and cosmetic industries.

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References

  1. T. Seal, Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves. Sonchus arvensis and Oenanthe linearis of North-Eastern region in India. J. App. Pharm. Sci 6, 157–166 (2016)

    Article  Google Scholar 

  2. N.V. Thomas, S.K. Kim, Potential pharmacological applications of polyphenolic derivatives from marine brown algae. Environ. Toxicol. Pharmacol. 32, 325–335 (2011)

    Article  CAS  Google Scholar 

  3. N. Lakshmi, Cytotaxonomical studies in eight genera of Amaryllidaceae. Cytologia 45, 663–673 (1980)

    Article  Google Scholar 

  4. N. Unver, New skeletons and new concepts in Amaryllidaceae alkaloids. Phytochem. Rev. 6, 125–135 (2007)

    Article  CAS  Google Scholar 

  5. E.A. Adewusi, G. Fouche, V. Steenkamp, Cytotoxicity and acetylcholinesterase inhibitory activity of an isolated crinine alkaloid from Boophane disticha (Amaryllidaceae). J. Ethnopharmacol 143, 572–578 (2012)

    Article  Google Scholar 

  6. E.A. Adewusi, V. Steenkamp, In vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from southern Africa. Asian Pac. J. Trop. Med. 4, 829–835 (2011)

    Article  CAS  Google Scholar 

  7. N. Rokbeni, Y. M’rabet, S. Cluzet, T. Richard, S. Krisa, M. Boussaid, A. Boulila, Determination of phenolic composition and antioxidant activities of Pancratium maritimum L. from Tunisia. Ind. Crops Prod. 94, 505–513 (2016)

    Article  CAS  Google Scholar 

  8. X. Li, M. Lu, D. Tang, Y. Shi, Composition of carotenoids and flavonoids in Narcissus cultivars and their relationship with flower color. PLoS ONE. 10, e0142074 (2015)

    Article  Google Scholar 

  9. A. Korkut, Research on variabilities in some important characters of Galanthus elwesii hook. Var. Elwesii grow under natural conditions. Acta Hortic 355, 189–194 (1994)

    Article  Google Scholar 

  10. A.P. Davis, J.R. Barnett, The leaf anatomy of the genus Galanthus L. (Amaryllidaceae J. St.-Hil.). Bot. J. Linn. Soc 123, 333–352 (1997)

    Google Scholar 

  11. E.J. Damme, H. Kaku, F. Perini, I.J. Goldstein, B. Peeters, F. Yagi, B. Decock, W.J. Peumans, Biosynthesis, primary structure and molecular cloning of snowdrop (Galanthus nivalis L.) lectin. Eur. J. Biochem. 202, 23–30 (1991)

    Article  Google Scholar 

  12. F. Yousefbeyk, B. Azadi, G. Amin, M. Salehi Sormaghi, M. Amini, M. Sharifzadeh, Phytochemical investigation of Galanthus transcaucasicus Fomin, as a source of isoquinoline alkaloids. Planta Med. 77, PG33 (2011)

    Google Scholar 

  13. M. Babashpour-Asl, H. Zakizadeh, H. Nazemiyeh, A. Motallebi-Azar, In vitro micropropagation and alkaloid production of Galanthus transcaucasicus Fomin. Pharm. Sci. 22, 267–271 (2016)

    Article  Google Scholar 

  14. A. Crozier, M.E. Lean, M.S. McDonald, C. Black, Quantitative analysis of the flavonoid content of commercial tomatoes, onions, lettuce, and celery. J. Agric. Food Chem. 45, 590–595 (1997)

    Article  CAS  Google Scholar 

  15. R. Hendra, S. Ahmad, E. Oskoueian, A. Sukari, M.Y. Shukor, Antioxidant, anti-inflammatory and cytotoxicity of Phaleria macrocarpa (Boerl.) Scheff fruit. BMC Complement. Altern. Med. 11, 1 (2011)

    Article  Google Scholar 

  16. H.I. Ismail, K.W. Chan, A.A. Mariod, M. Ismail, Phenolic content and antioxidant activity of cantaloupe (Cucumis melo) methanolic extracts. Food Chem. 119, 643–647 (2010)

    Article  CAS  Google Scholar 

  17. E. Karimi, H.Z. Jaafar, A. Ghasemzadeh, M. Ebrahimi, Fatty acid composition, antioxidant and antibacterial properties of the microwave aqueous extract of three varieties of Labisia pumila Benth. Biol. Res. 48, 9 (2015)

    Article  Google Scholar 

  18. M.A. Hossain, A. Rahman, Chemical composition of bioactive compounds by GC–MS screening and anti-fungal properties of the crude extracts of cabbage samples. Asian J. Biotechnol. 3, 68–76 (2011)

    Article  CAS  Google Scholar 

  19. I. Gulcin, I.G. Sat, S. Beydemir, M. Elmastas, O.I. Kufrevioglu, Comparison of antioxidant activity of clove (Eugenia caryophylata Thunb.) buds and lavender (Lavandula stoechas L.). Food Chem. 3, 393–400 (2004)

    Article  Google Scholar 

  20. G.C. Yen, P.D. Duh, Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species. J. Agric. Food Chem. 42, 629–632 (1994)

    Article  CAS  Google Scholar 

  21. E. Karimi, E. Oskoueian, R. Hendra, H.Z. Jaafar, Evaluation of Crocus sativus L. stigma phenolic and flavonoid compounds and its antioxidant activity. Molecules 5, 6244–6256 (2010)

    Article  Google Scholar 

  22. G.C. Yen, H.Y. Chen, Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agric. Food Chem. 43, 27–32 (1995)

    Article  CAS  Google Scholar 

  23. O. Boussaada, J. Chriaa, R. Nabli, S. Ammar, D. Saidana, M.A. Mahjoub, I. Chraeif, A.N. Helal, Z. Mighri, Antimicrobial and antioxidant activities of methanol extracts of Evax pygmaea (Asteraceae) growing wild in Tunisia. World J. Microbiol. Biotechnol. 24, 1289–1296 (2008)

    Article  Google Scholar 

  24. S. Khodadadi, T. Nejadsattari, A. Naqinezhad, M.A. Ebrahimzadeh, Diversity in antioxidant properties and mineral contents of Allium paradoxum in the Hyrcanian forests, Northern Iran. Biodiversitas 16, 281–287 (2015)

    Article  Google Scholar 

  25. M. Nikolova, R. Gevrenova, Determination of phenolic acids in amaryllidaceae species by high performance liquid chromatography. Pharm. Biol. 43, 289–291 (2005)

    Article  CAS  Google Scholar 

  26. D. Prakash, B.N. Singh, G. Upadhyay, Antioxidant and free radical scavenging activities of phenols from onion (Allium cepa). Food Chem. 102, 1389–1393 (2007)

    Article  CAS  Google Scholar 

  27. O. Ceylan, H. Alıc, Antibiofilm, antioxidant, antimutagenic activities and phenolic compounds of Allium orientale BOISS. Braz. Arch. Biol. Technnol. 58, 935–943 (2015)

    Article  CAS  Google Scholar 

  28. L. Jin, Y. Zhang, L. Yan, Y. Guo, L. Niu, Phenolic compounds and antioxidant activity of bulb extracts of six Lilium species native to China. Molecules 17, 9361–9378 (2012)

    Article  CAS  Google Scholar 

  29. R.M. Perez-Gregorio, M.S. García-Falcón, J. Simal-Gándara, A.S. Rodrigues, D.P. Almeida, Identification and quantification of flavonoids in traditional cultivars of red and white onions at harvest. J. Food Comp. Anal. 23, 592–598 (2010)

    Article  CAS  Google Scholar 

  30. M. Daglia, Polyphenols as antimicrobial agents. Curr. Opin. Biotechnol. 23, 174–181 (2012)

    Article  CAS  Google Scholar 

  31. T.P.T. Cushnie, A.J. Lamb, Antimicrobial activity of flavonoids. Int. J. Antimicrob. Agents 26, 343–356 (2005)

    Article  CAS  Google Scholar 

  32. A.I. Nazer, A. Kobilinsky, J.L. Tholozana, F. Dubois-Brissonneta, Combinations of food antimicrobials at low levels to inhibit the growth of Salmonella sv. Typhimurium: a synergistic effect? Food Micobiol. 22, 391–398 (2005)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Mashhad Branch, Islamic Azad University, Mashhad, Iran

    Ehsan Karimi & Masoud Homayouni-Tabrizi

  2. Department of Basic Science, Faculty of Crop Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

    Pooyan Mehrabanjoubani

  3. Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran

    Ahmad Abdolzadeh

  4. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

    Mozhgan Soltani

Authors
  1. Ehsan Karimi
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  2. Pooyan Mehrabanjoubani
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  3. Masoud Homayouni-Tabrizi
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  4. Ahmad Abdolzadeh
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  5. Mozhgan Soltani
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Correspondence to Ehsan Karimi.

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Karimi, E., Mehrabanjoubani, P., Homayouni-Tabrizi, M. et al. Phytochemical evaluation, antioxidant properties and antibacterial activity of Iranian medicinal herb Galanthus transcaucasicus Fomin. Food Measure 12, 433–440 (2018). https://doi.org/10.1007/s11694-017-9656-5

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  • DOI: https://doi.org/10.1007/s11694-017-9656-5

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