Abstract
Black pepper (Piper nigrum) is an essential tropical crop which doubled as spice in food industries and medicine in the treatment of free radical related disorders. For this study, microwave reflux method was employed in the extraction of bioactive oleoresin from black peppercorns. Extraction parameters such as irradiation time, microwave power, feed particle size and molar ratio were optimized. The effects of these parameters on the oleoresin yield and antiradical activities were investigated using a multi-level Taguchi methodology. The results obtained placed the optimum extraction condition at 120 min irradiation time, 350 W microwave power, 0.105 mm feed particle size and 12 g/ml molar ratio. Under this optimal condition, the oleoresin yield was obtained as 5.64% (w/w). However, the optimum percentage inhibitions of the extracted spice oleoresins on the stable DPPH and hydrogen peroxide radicals were estimated to be 88.75 and 90.31%, respectively. Moreover, the Fourier transform infrared (FTIR) analysis confirmed the presence of unsaturated amide groups, which validated the antioxidant potential of the black pepper extract. The scanning electronic microscopy (SEM) further elucidated the structural transformation of black pepper from the pulsed microwave heating effect.
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S.K.S. Rani, N. Saxena, Antimicrobial activity of black pepper (Piper nigrum L .). Global J. Pharmacol. 7, 87–90 (2013)
B. Hossein, M.Y. Manap, Z. Solati, Antioxidant activity of piper nigrum l. essential oil extracted by supercritical CO2 extraction and hydro-distillation. Talanta 121, 220–228 (2014)
N.H. Abdurahman, O.A. Olalere, Taguchi-based based optimization technique in reflux microwave extraction of piperine from black pepper (Piper nigrum). Aust. J. Basic. Appl. Sci. 10, 293–299 (2016)
G. Nahak, R.K. Sahu, Phytochemical evaluation and antioxidant activity of Piper cubeba and Piper nigrum. J. Appl. Pharm. Sci. 1, 153–157 (2011)
M. H. Mehmood, A. H. Gilani, Pharmacological basis for the medicinal use of black pepper and piperine in gastrointestinal disorders. J. Med. Food 13,1086–1096 (2010)
G. Raman, V. Gaikar, Microwave-assisted extraction of piperine from Piper nigrum. Ind. Eng. Chem. Res. 41, 2521–2528 (2002)
X. Cao, X. Ye, Y. Lu, W. Mo, Y. Yu, Ionic liquid-based ultrasonic-assisted extraction of piperine from white pepper. Anal. Chim. Acta 640, 47–51 (2009)
K. Vasavirama, M. Upender, Piperine: a valuable alkaloid from piper species. Int. J. Pharm. Pharm. Sci. 6, 34–38 (2014)
S. Devari, S. Jaglan, M. Kumar, R. Deshidi, S. Guru, S. Bhushan, M. Kushwaha, A.P. Gupta, S.G. Gandhi, J.P. Sharma, S.C. Taneja, R.A. Vishwakarma, B.A. Shah, Capsaicin production by Alternaria alternata, an endophytic fungus from Capsicum annum; LC-ESI-MS/MS analysis. Phytochemistry 98, 183–189 (2014)
P.D. Hamrapurkar, K. Jadhav, S. Zine, Quantitative estimation of piperine in Piper nigrum and Piper longum using high performance thin layer chromatography. J. Appl. Pharm. Sci. 1, 117–120 (2011)
S.R.S. Ferreira, M.A. Meireles, Modeling the supercritical fluid extraction of black pepper (Piper nigrum L.) essential oil. J. Food Eng. 54, 263–269 (2002)
B.A.S. Machado, C.G. Pereira, S.B. Nunes, F.F. Padilha, M.A. Umsza-Guez, Supercritical fluid extraction using CO2: main applications and future perspectives. Sep. Sci. Technol. 48, 2741–2760 (2013).
G. Raman, V.G. Gaikar, Micowave-assisted extraction of piperine from Piper nigrumr. Ind. Eng. Chem. Res 41, 2521–2528 (2002).
R. Vittal, A modified extraction and estimation method of oleoresin and piperine i black pepper (Piper nigrum le) berrie. Cent. Plant. Crop. Res. 3, 56–66 (1990)
A. Gupta, M. Gupta, S. Gupta, Isolation of piperine and few sesquiterpenes from the cold petroleum ether extract of Piper nigrum (black pepper) and its antibacterial activity. Int. J. Pharmacol. Phytochem. Res. 5, 3–7 (2013)
L.L. Zhang, J.G. Xu, Comparative study on antioxidant activity of essential oil. Eur. J. Food Sci. Technol. 3, 10–16 (2015)
N.H. Abdurahman, O.A. Olalere, A comparative review of conventional and microwave assisted extraction in capsaicin isolation from chili pepper. Aust. J. Basic. Appl. Sci. 10, 263–275 (2016)
O.A. Olalere, N.H. Abdurahman, R.O. Alara, O.A. Habeeb, Parametric optimization of microwave reflux extraction of spice oleoresin from white pepper (Piper nigrum). J. Analyt. Sci. Technol. 8, 1–8 (2017)
M.A. Desai, J. Parikh, Extraction of essential oil from leaves of lemongrass using microwave radiation: optimization, comparative, kinetic, and biological studies. Sustain. Chem. Eng. 3, 421–431 (2015)
C. Chan, J. Lim, R. Yusoff, G. Ngoh, A generalized energy-based kinetic model for microwave-assisted extraction of bioactive compounds from plants. Sep. Purif. Technol. 143, 152–160 (2015)
J. Anwar, U. Shafique, R. Waheed-uz-Zaman, Rehman, M. Salman, A. Dar, J.M. Anzano, U. Ashraf, S. Ashraf, Microwave chemistry: effect of ions on dielectric heating in microwave ovens. Arab. J. Chem. 8, 100–104 (2015)
R. Lokhande, A. Deshpande, A. Mache, Taguchi analysis of bonded single-lap joint in hemp fiber composite. Int. J. Eng. Tech. Sci. 5, 27–33 (2016)
V. Mandal, Y. Mohan, S. Hemalatha, Microwave assisted extraction of curcumin by sample-solvent dual heating mechanism using Taguchi L9 orthogonal design. J. Pharm. Biomed. Anal. 46, 322–327 (2008)
O. Odeja, C.E. Ogwuche, E.E. Elemike, G. Obi, Phytochemical screening, antioxidant and antimicrobial activities of Acalypha ciliata plant. Clin. Phytosci. 2, 12 (2017)
S. Singh, I.P.S. Kapoor, G. Singh, C. Schuff, M.P. De Lampasona, C.A.N. Catalan, Chemistry, antioxidant and antimicrobial potentials of white pepper (Piper nigrum L.) essential oil and oleoresins. Proc. Natl. Acad. Sci. India Sect. B 83, 357–366 (2013)
M.R. Thakker, J.K. Parikh, M.A. Desai, Microwave assisted extraction of essential oil from the leaves of Palmarosa: multi-response optimization and predictive modelling. Ind. Crops Prod. 86, 311–319 (2016)
S. Maghsoodloo, G. Ozdemir, V. Jordan, C.H. Huang, Strengths and limitations of Taguchi’s contributions to quality, manufacturing, and process engineering. J. Manuf. Syst. 23, 73–126 (2004)
E. Subroto, R. Manurung, H.J. Heeres, A.A. Broekhuis, Optimization of mechanical oil extraction from Jatropha curcas L. kernel using response surface method. Ind. Crops Prod. 63, 294–302 (2015)
M. Gallo, Quantitative methods in regional science measuring passenger satisfaction: a strategy based on rasch analysis and the anom. J. Appl. Quant. Methods 6, 27–35 (2011)
M.K. Abbass, S.K. Hussein, A.A. Khudhair, Optimization of mechanical properties of friction stir spot welded joints for dissimilar aluminum alloys (AA2024-T3 and AA 5754-H114). Arab. J. Sci. Eng. 4,1–4 (2016)
Y. Yuan, D. Macquarrie, Microwave assisted extraction of sulfated polysaccharides (fucoidan) from Ascophyllum nodosum and its antioxidant activity. Carbohydr. Polym. 129, 101–107 (2015)
L. S. Badwaik, P.K. Borah, S.C. Deka, Optimization of microwave assisted extraction of antioxidant extract from Garcinia pedunculata robx. Sep. Sci. Technol. 50, 1814–1822 (2015)
G. Raman, V.G. Gaikar, Extraction of piperine from Piper nigrum (black pepper) by hydrotropic solubilization. Ind. Eng. Chem. Res. 41, 2966–2976 (2002).
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The authors wish to acknowledge the financial support through the research grant (PGRS-160320) from the Universiti Malaysia Pahang, Malaysia.
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Olalere, O.A., Abdurahman, N.H. & Alara, O.R. Extraction, radical scavenging activities and physicochemical fingerprints of black pepper (Piper nigrum) extract. Food Measure 11, 2195–2201 (2017). https://doi.org/10.1007/s11694-017-9604-4
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DOI: https://doi.org/10.1007/s11694-017-9604-4