Skip to main content
SpringerLink
Log in

Magnetoelastic biosensor for the detection of Salmonella typhimurium in food products

  • Original Paper
  • Published:
Sensing and Instrumentation for Food Quality and Safety Aims and scope Submit manuscript

Abstract

In this article, a magnetoelastic sensor immobilized with polyclonal antibody for the detection of Salmonella typhimurium in food products is described. The remote query nature of magnetoelastic sensors enables the detection of bacterial species in sealed and opaque containers. Bacterial binding to the antibody on the sensor surfaces changed the resonance parameters, and these changes were quantified by the shift in the sensor’s resonance frequency. Response of the sensors to increasing concentrations (5 × 101–5 × 108 cfu/ml) of S. typhimurium in three different food products (water, fat-free milk and apple juice) was studied and similar responses were observed. These results were also further ascertained by Scanning Electron Microscopy (SEM) studies. A detection limit of 5 × 103 cfu/ml, with a sensitivity of 139 Hz/decade was obtained for the sensors tested in water samples, as compared to 129 Hz/decade in apple juice and 127 Hz/decade in fat free milk. A 2 × 0.4 × 0.015 mm sensor was employed in all the investigations. The dissociation constant K d and the binding valencies for S. typhimurium spiked in water samples was 435 cfu/ml and 2.33 respectively; as compared to 309 cfu/ml and 2.38 for apple juice; and 1389 cfu/ml and 1.85 for fat free milk samples. Bacterial binding was specific and a divalent binding was observed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. P.S. Mead, L. Slutsker, V. Dietz, D. Dietz, L.F. McCaig, Emerging Infectious Diseases 5, 607 (1999)

    Google Scholar 

  2. M.E. Potter, S. Gonzalez-Ayala, N. Silarug, Food Microbiology: Fundamentals and Frontiers (ASM Press, Washington, DC, 1997)

  3. G.W. Beran, H.P. Shoeman, K.F. Anderson, Dairy Food Environ. Sanit. 11, 189 (1991)

    Google Scholar 

  4. B. Swaminathan, P. Feng, Annu. Rev. Microbiol. 48, 401 (1994)

    Google Scholar 

  5. M.L. Ackers, S. Schoenfeld, J. Markman et al., J. Infect. Dis. 181, 1834 (2000)

    Article  Google Scholar 

  6. C.A. Ryan, M.K. Nickels, N.T. Hargrett-Bean et al., JAMA 258, 3269 (1987)

    Article  CAS  Google Scholar 

  7. C.B. Dalton, C.C. Austin, J. Sobel et al., N. Engl. J. Med. 336, 100 (1997)

    Article  CAS  Google Scholar 

  8. MMWR 54, 325 (2005)

  9. F. Jones, D. Rives, J. Carey, Poult. Sci. 74 (1995)

  10. C.M. Schroeder, A.L. Nangle, W.D. Schlosser, A.T. Hogue et al., Emerging Infectious Diseases 11, 113 (2005)

    Google Scholar 

  11. MMWR 48, 582 (1999)

  12. MMWR Morb. Mortal. Wkly. Rep. 24, 413 (1975)

    Google Scholar 

  13. MMWR Morb Mortal Wkly Rep. 28, 117 (1979)

  14. MMWR Morb Mortal Wkly Rep. 33, 505 (1984)

  15. M. Layton, S. Calliste, T. Gomez, C. Patton, S. Brooks, Infect. Control. Hosp. Epidemiol. 18, 115 (1997)

    Article  CAS  Google Scholar 

  16. M. Mahony, H. Barnes, R. Stanwell-Smith, T. Dickens, A. Jephcott, J. Public. Health. Med. 12, 19 (1990)

    Google Scholar 

  17. G.H. Snoeyenbos, C.F. Smyser, H. Van Roekel, Avian. Dis. 13, 668 (1969)

    Article  CAS  Google Scholar 

  18. R. Bokanyi, J. Stephens, D. Foster, Poult. Sci. 69, 592 (1990)

    Google Scholar 

  19. MMWR 55, 180 (2006)

  20. R. Fontaine, M. Cohen, W. Martin, T. Vernon, Am. J. Epidemiol. 111, 247 (1980)

    CAS  Google Scholar 

  21. K.R. Rogers, Analytica. Chimica. Acta. 568, 222 (2006)

    Article  CAS  Google Scholar 

  22. H.J. Beckers, P.D. Tips, P.S.S. Soentoro, E.H.M. Delfgou-Van Asch, R. Peters, Food. Microbiol. 5, 147 (1998)

    Article  Google Scholar 

  23. S.D. Oliveira, L.R. Santos, D.M.T. Schuch et al., Vet. Microbiol. 87, 25 (2002)

    Article  CAS  Google Scholar 

  24. E.V. Olsen, S.T. Pathirana, A.M. Samoylov, J.M. Barbaree, B.A. Chin, W.C. Neely, V. Vodyanoy, J. Microbiol. Methods 53, 273 (2003)

    Article  CAS  Google Scholar 

  25. V. Nanduri, I.B. Sorokulova, A.M. Samoylov et al., Biosensors and Bioelectronics 22, 986 (2007)

    Article  CAS  Google Scholar 

  26. T.B. Tims, D.V. Lim, J. Microbiol. Methods 59, 127 (2004)

    Article  CAS  Google Scholar 

  27. H.L. Bandey, R.W. Cernosek, W.E. Lee, L.E. Ondrovic, Biosens. Bioelectrons. 19, 1657 (2004)

    Article  CAS  Google Scholar 

  28. N. Bouropoulos, D. Kouzoudis, C.A. Grimes, Sens. Actuators B 109, 227 (2005)

    Article  CAS  Google Scholar 

  29. K. Shankar, K. Zeng, C. Ruan, C.A. Grimes, Sens. Actuators B 107, 640 (2005)

    Article  CAS  Google Scholar 

  30. S. Wu, Y. Zhu, Q. Cai, K. Zeng, C.A. Grimes, Sens. Actuators B doi: 10.1016/j.snb.2006.04.095 (2006)

  31. K.G. Ong, J.M. Leland, K. Zeng, G. Barrett, M. Zourob, C.A. Grimes, Biosens. Bioelectrons. 21, 2270–2274 (2006)

    Article  CAS  Google Scholar 

  32. http://metglas.com/products/page5_1_2_7.htm, September, 2006

  33. S.T. Pathirana, J. Barbaree, B.A. Chin, M.G. Hartell, W.C. Neely, V. Vodyanoy, Biosens. Bioelectron 15, 135 (2000)

    Article  CAS  Google Scholar 

  34. P.G. Stoyanov, C.A. Grimes, Sen. Actuators A 80, 8 (2000)

    Article  CAS  Google Scholar 

  35. Q.Y. Cai, A. Cammers-Goodwin C.A. Grimes, J. Environ. Monit. 2, 556 (2000)

    Article  CAS  Google Scholar 

  36. V. Nanduri, G. K. Mark, T. Morgan et. al., Sensors 6, 808 (2006)

  37. I.H. Segel, A.H. Segel, Biochemical Calculations (Wiley, New York, 1976)

  38. V.A. Petrenko, V.J. Vodyanoy, J. Microbiol. Methods 53, 253 (2003)

    Article  CAS  Google Scholar 

  39. R. Guntupalli, J. Hu, R.S. Lakshmanan, T.S. Huang, J.M. Barbaree, B.A. Chin, Biosens. Bioelectrs. doi:10.1016/j.bios.2006.06.037 (2006)

  40. L. Ye, S.V. Letcher, A.G. Rand, J. Food Sci. 62, 1067 (1997)

    Article  CAS  Google Scholar 

  41. J.C. Pyun, H. Beutel, J.U. Meyer, H.H. Ruf, Biosens. Bioelectrons. 13, 839 (1998)

    Article  Google Scholar 

  42. I.S. Park, N. Kim, Biosens. Bioelectrons. 13, 1091 (1998)

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This project was supported by USDA grant 2005-3439415674A.

Author information

Authors and Affiliations

  1. Materials Research and Education center, Auburn University, Auburn, AL, 36849, USA

    Rajesh Guntupalli, Ramji S. Lakshmanan, Michael L. Johnson, Jing Hu & Bryan A. Chin

  2. Department of Nutrition and Food Science, Auburn University, Auburn, AL, 36849, USA

    Tung-Shi Huang

  3. Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA

    James M. Barbaree

  4. Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL, 36849, USA

    Vitaly J. Vodyanoy

Authors
  1. Rajesh Guntupalli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ramji S. Lakshmanan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michael L. Johnson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jing Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tung-Shi Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. James M. Barbaree
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Vitaly J. Vodyanoy
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Bryan A. Chin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bryan A. Chin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Guntupalli, R., Lakshmanan, R.S., Johnson, M.L. et al. Magnetoelastic biosensor for the detection of Salmonella typhimurium in food products. Sens. & Instrumen. Food Qual. 1, 3–10 (2007). https://doi.org/10.1007/s11694-006-9003-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11694-006-9003-8

Keywords

Search

Navigation