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Phylogenetic and spectroscopic analysis of Alicyclobacillus isolates by 16S rDNA sequencing and mid-infrared spectroscopy

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Abstract

Alicyclobacillus spp. are a group of thermophilic, acidophilic, spore-forming bacteria, some of which cause spoilage in commercially pasteurized fruit and vegetable juice products including apple, orange, tomato and carrot juice. In this study, we characterized seven isolates of Alicyclobacillus (14-2, KF, A-Gala 2-1, C-Fuji 6, Gala 9-2, 1016, 18-1) and a reference strain A. acidiphilus DSM 14558 by a 16S rDNA sequencing technique and Fourier transform infrared (FT-IR) spectral features. The degree of similarity between Alicyclobacillus isolates based upon phylogenetic analysis of sequence data and multivariate statistical analysis of FT-IR spectral data was determined. Results from both methods were consistent, suggesting that a combination of methods targeting both 16S rDNA and mid-infrared spectroscopic signatures may be a suitable and more accurate approach to either identify or discriminate Alicyclobacillus isolates from fruit and vegetable juice products.

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References

  1. S.-Y. Lee, R.H. Dougherty, D.-H. Kang, Appl. Environ. Microbiol. 68, 4158 (2002)

    Article  CAS  Google Scholar 

  2. H. Matsubara, K. Goto, T. Matsumura, et al., Int. J. Syst. Evol. Microbiol. 52, 1681 (2002)

    Article  CAS  Google Scholar 

  3. G.L. Pettipher, M.E. Osmundson, J.M. Murphy, Lett. Appl. Microbiol. 24, 185 (1997)

    Article  CAS  Google Scholar 

  4. F.M. Silva, P. Gibbs, M.C. Vieira, C.L.M. Silva, Int. J. Food Microbiol. 51, 95 (1999)

    Article  CAS  Google Scholar 

  5. M. Sinigaglia, M.R. Corbo, C. Altieri, D. Campaniello, D. D’Amato, A. Bevilacque, J. Food Prot. 66, 2216 (2003)

    Google Scholar 

  6. J.D. Wisotzkey, P. Jurtshuk, G.E. Fox, G. Deinhard, K. Poralla, Int. J. Syst. Bacteriol. 44, 263 (1992)

    Google Scholar 

  7. T.A. Eisele, M.J. Semon, J. Food Sci. 70, 267 (2005)

    Article  Google Scholar 

  8. G.L. Pettipher, M.E. Osmundson, Food Australia 57, 293 (2000)

    Google Scholar 

  9. M. Murakami, H. Tedzuka, K. Yamazaki, Food Microbiol. 15, 577 (1998)

    Article  Google Scholar 

  10. K. Yamazaki, T. Okubo, N. Inoue, H. Shinano, Biosci. Biotechnol. Biochem. 61, 1016 (1997)

    Article  CAS  Google Scholar 

  11. C.-K. Lin, C.-L. Hung, S.-C. Hsu, C.-C. Tsai, H.-Y. Tsen, J. Food Prot. 67, 1335 (2004)

    CAS  Google Scholar 

  12. H. Oberreuter, H. Seiler, S. Scherer, Int. J. Syst. Evol. Microbiol. 52, 91 (2002)

    CAS  Google Scholar 

  13. H. Oberreuter, J. Charzinski, S. Scherer, Microbiol. 148, 1523 (2002)

    CAS  Google Scholar 

  14. D. Xu, J.-C. Côté, Int. J. Syst. Evol. Microbiol. 53, 695 (2003)

    Article  CAS  Google Scholar 

  15. F.V. von Stetten, K.P. Francis, S. Lechner, K. Neuhaus, S. Scherer, J. Microbiol. Meth. 34, 99 (1998)

    Article  Google Scholar 

  16. A.H. Farnleitner, F. Zibuschka, M.M. Burtscher, G. Lindner, G. Reischer, R.L. Mach, Int. J. Food Microbiol. 92, 333 (2004)

    Article  CAS  Google Scholar 

  17. Y. Song, C. Liu, M. Bolanos, J. Lee, M. McTeague, S.M. Finegold, J. Clin. Microbiol. 43, 1531 (2005)

    Article  CAS  Google Scholar 

  18. M. Kansiz, P. Heraud, B. Wood, F. Burden, J. Beardall, D. McNaughton, Phytochemistry 52, 407 (1999)

    Article  CAS  Google Scholar 

  19. V. Lucia, B. Daniela, L. Rosalba, Int. J. Food Microbiol. 69, 113 (2001)

    Article  CAS  Google Scholar 

  20. S.F. Lin, J.H. Schraft, M.W. Griffiths, J. Food Prot. 61, 921 (1998)

    CAS  Google Scholar 

  21. M. Al-Holy, M. Lin, A.G. Cavinato, B.A. Rasco, Food Microbiol. 23, 162 (2006)

    Article  CAS  Google Scholar 

  22. D. Naumann, in Proceedings of SPIE, vol. 3257, 1998, p. 245, ed. by H. H. Mantsch, M. Jackson

  23. M. Lin, M. Al-Holy, S.-S. Chang, A.G. Cavinato, D.-H. Kang, B.A. Rasco, Int. J. Food Microbiol. 105, 369 (2005)

    Article  CAS  Google Scholar 

  24. S.-S. Chang, Master thesis, Washington State University (2003)

  25. M. Lin, A.G. Cavinato, Y. Huang, B.A. Rasco, Food Res. Int. 36, 761 (2003)

    Article  CAS  Google Scholar 

  26. E. Stackebrandt, B.M. Goebel, Int. J. Syst. Bacteriol. 44, 846 (1994)

    Article  CAS  Google Scholar 

  27. K. Goto, K. Mochida, M. Asahara, M. Suzuki, H. Kasai, A. Yokota, Int. J. Syst. Evol. Microbiol. 53, 1537 (2003)

    Article  CAS  Google Scholar 

  28. L.P. Choo-Smith, K. Maquelin, T.V. Vreeswijk, et al., Appl. Environ. Microbiol. 67, 1461 (2001)

    Article  CAS  Google Scholar 

  29. J. Schmitt, H.-C. Flemming, Int. Biodeterior. Biodegrad. 41, 1 (1998)

    Article  CAS  Google Scholar 

  30. K. Maquelin, C. Kirschner, L.-P. Choo-Smith, et al., J. Microbiol. Meth. 51, 255 (2002)

    Article  CAS  Google Scholar 

  31. W. Zeroual, C. Choisy, S.M. Doglia, J.H. Bobichon, J.R. Angiboust, M. Manfait, Biochim. Biophys. Acta 112, 171 (1994)

    Google Scholar 

  32. K.C. Schuster, E. Urlaub, J.R. Gapes, J. Microbiol. Meth. 42, 29 (2000)

    Article  CAS  Google Scholar 

  33. D.J.M. Mouwen, M.J.B.M. Weijtens, R. Capita, C. Alonso-Calleja, M. Prieto, Appl. Environ. Microbiol. 71, 4318 (2005)

    Article  CAS  Google Scholar 

  34. A.F. Schmalreck, P. Trankle, E. Vanca, R. Blaschke-Hellmessen, Mycoses 41, 71 (1998)

    Article  CAS  Google Scholar 

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Acknowledgment

Special thanks are given to Tovah Kerr in the Department of Animal Science, and to Peter Gray and Dewi Sediaty in the Department of Food Science and Human Nutrition at Washington State University for their assistance in this study.

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Authors and Affiliations

  1. Food Science Program, University of Missouri, 256 William Stringer Wing, Eckles Hall, Columbia, MO, 65211, USA

    Mengshi Lin

  2. Department of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite University, Zarqa, Jordan

    Murad Al-Holy

  3. Department of Nutrition and Food Technology, The University of Jordan, Amman, Jordan

    Hamzah Al-Qadiri

  4. Department of Food Science and Human Nutrition, Washington State University, Box 646376, Pullman, WA, 99164, USA

    Su-Sen Chang, Dong-Hyun Kang & Barbara A. Rasco

  5. Department of Animal Science, Washington State University, Box 646310, Pullman, WA , 99164, USA

    Buel D. Rodgers

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  1. Mengshi Lin
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  2. Murad Al-Holy
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  3. Hamzah Al-Qadiri
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  4. Su-Sen Chang
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  7. Barbara A. Rasco
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Correspondence to Mengshi Lin.

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Lin, M., Al-Holy, M., Al-Qadiri, H. et al. Phylogenetic and spectroscopic analysis of Alicyclobacillus isolates by 16S rDNA sequencing and mid-infrared spectroscopy. Sens. & Instrumen. Food Qual. 1, 11–17 (2007). https://doi.org/10.1007/s11694-006-9000-y

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  • DOI: https://doi.org/10.1007/s11694-006-9000-y

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