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Genetic diversity and differentiation of the Dybowski’s frog (Rana dybowskii) in Northeast China

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Abstract

The genetic diversity and population structure of the Dybowski’s frog (Rana dybowskii) were investigated by using 11 polymorphic microsatellite loci. Total 75 individuals were sampled from six populations in Lesser Khingan Mountains and Changbai Mountains, China. Results showed that allele number of the 11 microsatellite loci was in the range of 2–10 in all populations, with the mean of 5.6. The average expected heterozygosity (H E) was 0.572, indicating a moderate polymorphism. The results of genetic differentiation coefficient (F ST) showed that population genetic differentiation was significant between Changbai and Lesser Khingan Mountains (p<0.001). This result was verified further by Nei’s genetic distance (D A) based on UPGMA phylogenetic trees and by AMOVA analysis. In conclusion, the populations distributed in Lesser Khingan Mountains and Changbai Mountain are proposed to be two distinct management units (MUs) for their protection and management.

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Authors and Affiliations

  1. College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, P. R. China

    Ming Zhang, Yi-dan Ma & Jian-zhang Ma

  2. Laboratory of Medical Genetics, Harbin Medical University, Harbin, 150081, P. R. China

    Xue-yuan Jia

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  1. Ming Zhang
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  2. Xue-yuan Jia
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  3. Yi-dan Ma
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  4. Jian-zhang Ma
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Correspondence to Jian-zhang Ma.

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Zhang, M., Jia, Xy., Ma, Yd. et al. Genetic diversity and differentiation of the Dybowski’s frog (Rana dybowskii) in Northeast China. Journal of Forestry Research 21, 239–245 (2010). https://doi.org/10.1007/s11676-010-0039-6

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  • DOI: https://doi.org/10.1007/s11676-010-0039-6

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