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A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on endothelial cells

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Abstract

Objective

To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction (血府逐瘀汤) on the endothelial cell line ECV304.

Methods

ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoctioncontaining serum (XFZYD-CS) for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide (MTT), fluorescence activating cell sorter (FACS), migration, adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction (RT-PCR) superarray.

Results

At 48 h, XFZYD-CS induced ECV304 significantly improved cell viability, number in S phase, migration, adhesion and tube formation. At 24 h and 72 h, only cell migration was elevated. Microarray results showed that the expression of 27 angiogenesis-related genes was changed.

Conclusion

XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h.

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Authors and Affiliations

  1. Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, 100700, China

    Jun Song  (宋 军)

  2. Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350108, China

    Wen-yuan Chen  (陈文元), Li-ya Wu  (吴立娅) & Dong Gao  (高 冬)

  3. Fujian Academy of Integrative Medicine, Fuzhou, 350108, China

    Liang-pu Zheng  (郑良朴) & Wei Lin  (林 薇)

  4. Harvard Medical School, Boston, MA, 02215, USA

    Ted J. Kaptchuk

  5. Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China

    Ke-ji Chen  (陈可冀)

Authors
  1. Jun Song  (宋 军)
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  2. Wen-yuan Chen  (陈文元)
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  3. Li-ya Wu  (吴立娅)
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  4. Liang-pu Zheng  (郑良朴)
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  5. Wei Lin  (林 薇)
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  6. Dong Gao  (高 冬)
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  7. Ted J. Kaptchuk
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  8. Ke-ji Chen  (陈可冀)
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Corresponding author

Correspondence to Dong Gao  (高 冬).

Additional information

Supported by the National Natural Science Foundation of China (No. 81072933 and No. 81173431); Fujian Natural Science Foundation (No. 2007J0101); Fujian Academy of Integrative Medicine Foundation (No. CKJ2008001); National Institutes of Health—The National Center for Complementary and Alternative Medicine Foundation of US (NIH-NCCAM No. K24 AT004095)

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Song, J., Chen, Wy., Wu, Ly. et al. A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on endothelial cells. Chin. J. Integr. Med. 18, 502–506 (2012). https://doi.org/10.1007/s11655-012-1143-6

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  • DOI: https://doi.org/10.1007/s11655-012-1143-6

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