Abstract
Objective
To observe the effect of Xuefu Zhuyu Decoction (血府逐瘀汤)-containing serum (XFZYD-CS) on endothelial progenitor cell (EPC) tube formation in vitro.
Methods
Mononuclear cells from rat bone marrow were prepared in a Ficoll density gradient centrifuge. EPCs were separated by the differential attachment method, and observed with inverted microscope for the effect of XFZYD-CS on EPC tube formation.
Results
After one day, EPCs exposed to the serum containing 5%, 10% and 15% XFZYD-CS formed typical tubes or vessel networks. The tube formation time was two days ahead of the control group and the size of most tubes in the serum groups was smaller than in the control group.
Conclusion
XFZYD-CS could induce EPC angiogenesis and hasten tube formation, especially in capillary vessels. The study provides experimental evidence for the plausibility of Xuefu Zhuyu Decoction in the treatment of ischemic diseases.
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Supported by the National Natural Science Foundation of China (No. 30772877); Basic Research Fund of China Academy of Chinese Medical Sciences (No. ZZ2006039); Fujian Academy of Integrative Medicine Foundation (No. 3000-905010805)
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Gao, D., Wu, Ly., Jiao, Yh. et al. The effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on in vitro endothelial progenitor cell tube formation. Chin. J. Integr. Med. 16, 50–53 (2010). https://doi.org/10.1007/s11655-010-0050-y
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DOI: https://doi.org/10.1007/s11655-010-0050-y