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Effects of Huanshuai Recipe Oral Liquid (缓衰口服液) on restructuring glomerular microvasculature and expression of vascular endothelial growth factor in subtotal nephrectomized rats

  • Experimental Research
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Abstract

Objectives

To explore the effects and significance of Huanshuai Recipe Oral Liquid (缓衰口服液, HSR), a formula with supplementing qi, nourishing blood and activating blood on restructuring glomerular microvasculature and expression of vascular endothelial growth factor (VEGF) in subtotal nephrectomized (SNX) rats.

Methods

A total of 76 male Wistar rats were randomly divided into four groups: 16 in the sham-operated group and fed with tap water 10 mL/kg per day; 20 in the model group were operated with 5/6 SNX and fed with tap water 10 mL/ kg per day; 20 SNX rats in the HSR group were treated with HSR 10 mL/kg per day; 20 SNX rats in the losartan group were treated with losartan 40 mg/kg per day. Serum creatinine (SCr) and urinary protein excretion (Upro) were examined at the 2nd, 4th, 8th, and 12th weeks of the treatment, and the remnant kidneys were harvested. Changes in histological microstructure were evaluated using light microscopy, and the expression of VEGF was detected by using ELISA.

Results

Upro, microvasculature injury and glomerulosclerosis were found to be alleviated in HSR and Losartan groups, respectively. The change of VEGF expression showed positive correlation with glomerular capillary area and peritubular capillary number (r=0.448, r=0.422, P<0.01), but negative correlation with that of SCr and Upro (r=−0.592, r=−0.481, P<0.01).

Conclusions

HSR could regulate the VEGF expression, reduce the loss of microvasculature, which demonstrated similar renal protective effects to losartan in SNX rats. Examination of Chinese herbal medicine influence on VEGF signaling and restructuring renal microvasculature may elucidate the molecular mechanism of renal protection to a certain degree.

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Authors and Affiliations

  1. Guang’ anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, 100053, China

    Shen Li  (李 深) & Xiang-rong Rao  (饶向荣)

  2. Department of Renal Nephrology, Peking University First Hospital, Institute of Nephrology, Peking University and Key Lab of Public Health Ministry of China, Beijing, 100034, China

    Jin-ye Song  (宋锦叶), Li-qiang Meng  (孟立强), Lei Qu  (屈 磊) & Xiao-mei Li  (李晓玫)

Authors
  1. Shen Li  (李 深)
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  2. Xiang-rong Rao  (饶向荣)
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  3. Jin-ye Song  (宋锦叶)
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  4. Li-qiang Meng  (孟立强)
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  6. Xiao-mei Li  (李晓玫)
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Correspondence to Shen Li  (李 深).

Additional information

Supported by Special Projects of Chinese Medicine Science and Technology Research, State Administration of Traditional Chinese Medicine (No. 04-05JQ07)

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Li, S., Rao, Xr., Song, Jy. et al. Effects of Huanshuai Recipe Oral Liquid (缓衰口服液) on restructuring glomerular microvasculature and expression of vascular endothelial growth factor in subtotal nephrectomized rats. Chin. J. Integr. Med. 16, 239–246 (2010). https://doi.org/10.1007/s11655-010-0239-4

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  • DOI: https://doi.org/10.1007/s11655-010-0239-4

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