Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium

Abstract

Objective

To investigate the effect of Jinmaitong (筋脉通, JMT) serum on the proliferation of rat Schwann cells (SCs) primarily cultured in high glucose medium.

Method

SCs were primarily cultured in Dulbecco’s minmum essential medium (DMEM control), 50 mmol/L glucose medium (50 mmol/L Glu), 75 mmol/L glucose medium (75 mmol/L Glu), as well as 50 mmol/L glucose medium, with different concentrations of JMT serum (undiluted, 1:2 diluted and 1:8 diluted) and Neurotropin (Ntp), respectively. The proliferation of SCs under different conditions was detected by MTT.

Result

SCs grew exuberantly in DMEM within 24–72 h, but slowed down at 96 h. The proliferation of SCs was inhibited in 50 mmol/L Glu and 75 mmol/L Glu after cultures of 48, 72 and 96 h, which showed that both were significantly different compared to the control group (P<0.01). The inhibition was more significant in 75 mmol/L Glu than in 50 mmol/L Glu (P<0.05). Spearman’s rho analysis revealed that the proliferation of SCs had a negative correlation with the concentration of glucose (r=−0.471, P<0.01). Excluding the time factor, partial correlation showed similar results (r=−0.679, P<0.01). After 48 h, the proliferation of SCs increased significantly in JMT1:2 and Ntp compared with 50 mmol/L Glu (control 0.437±0.019, 50 mmol/L Glu 0.367±0.035, JMT1:2 0.426±0.024, Ntp 0.422±0.013; P<0.01), and there were no statistically significant differences among the JMT groups, the Ntp group and the control group (P>0.05).

Conclusions

The proliferation of SCs was inhibited in high glucose medium, and the inhibition was reduced by different concentrations of JMT serum, especially at JMT1:2.