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Practical methodology for gametophyte proliferation and sporophyte production in green penny fern (Lemmaphyllum microphyllum C. Presl) using mechanical fragmentation

  • Micropropagation
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Abstract

Propagation of gametophytes and sporophytes using mechanical fragmentation has been considered a suitable method for mass production of ferns. This study aimed to develop a practical propagation method for Lemmaphyllum microphyllum C. Presl, which is a fern of significant ornamental and medicinal value. Gametophytes were obtained through in vitro spore germination and used for propagation experiments. The gametophyte was mechanically fragmented using a scalpel into small fragments, which were then used to investigate gametophyte proliferation. In addition, the gametophyte was fragmented using a blender and then used to study sporophyte formation. Optimal proliferation conditions of the gametophyte were determined using Murashige and Skoog (MS) basal medium (double-, full-, half-, quarter-strength), Knop medium, and medium components (sucrose, nitrogen sources, activated charcoal), at various concentrations. The fresh weight of the gametophyte was 14-fold higher than that of gametophytes (300 mg) used as culture material, when cultured on double-strength MS. Moreover, 1 g of the gametophyte fragmented in 25 mL of distilled water formed more than 430 sporophytes in a soil mixture in an area of 7.5 cm2. The sporophytes were successfully cultivated in the greenhouse after acclimation. A large-scale production method for L. microphyllum that can be easily implemented in a fern production farm is outlined.

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Acknowledgments

This study was supported by the “useful plant collection and development of mass propagation protocol for the establishment of the foundation of convergence platform using forest plants (KNA1-2-25, 16-3)” project funded by Korea National Arboretum.

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Author notes

  1. Bo Kook Jang and Ju Sung Cho have contributed equally to this work.

Authors and Affiliations

  1. Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, Republic of Korea

    Bo Kook Jang, Ju Sung Cho, Kyungtae Park & Cheol Hee Lee

  2. Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University, Cheongju, Republic of Korea

    Bo Kook Jang, Ju Sung Cho, Kyungtae Park & Cheol Hee Lee

Authors
  1. Bo Kook Jang
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  2. Ju Sung Cho
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  3. Kyungtae Park
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  4. Cheol Hee Lee
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Correspondence to Cheol Hee Lee.

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Editor: Masaru Nakano

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Jang, B.K., Cho, J.S., Park, K. et al. Practical methodology for gametophyte proliferation and sporophyte production in green penny fern (Lemmaphyllum microphyllum C. Presl) using mechanical fragmentation. In Vitro Cell.Dev.Biol.-Plant 56, 318–324 (2020). https://doi.org/10.1007/s11627-020-10055-2

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  • DOI: https://doi.org/10.1007/s11627-020-10055-2

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