Abstract
The effects of microorganisms on the health and growth of tissue-cultured plants is not well studied. In the current study, treatment of tissue culture-raised Musa acuminata cv. Grand Naine plants with culture supernatants of Pseudomonas putida, Pseudomonas flourescens, and Bacillus sp. had beneficial effects on acclimatization and hardening. At the hardening phase, there was an enhancement of various growth parameters of plants treated with microbial culture supernatants from the first week onwards. Interestingly, after a period of 5 wk, the biohardened plants showed significant improvement in number of roots, length of roots, number of root branches, length of shoot, number of leaves, leaf length, and leaf width. Most remarkably, microbial treatments shortened the hardening period to 5 wk, which otherwise could have taken about 10 wk. The results indicate that selected plant-associated microorganisms, particularly Bacillus sp., have the potential to augment acclimatization and to improve the quality of plants during the hardening process. Reduction in the length of the hardening period is very important as it translates into process cost reduction and a resultant reduction in per plant cost. Hence, the outcome of the study has commercial and agricultural applications.
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Acknowledgments
EK Radhakrishnan is thankful to the Department of Biotechnology (DBT), Govt. of India under DBT-RGYI and DBT-BUILDER/MSUB schemes (BT/PR4800/INF/22/152/2012 dated 23/3/2012), and to the Kerala State Council for Science, Technology & Environment (KSCSTE), Govt. of Kerala, under the KSCSTE-SARD support programme for financial assistance. A Remakanthan is thankful to KSCSTE, Government of Kerala, for funding for a research project in YIPB scheme.
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Editor: Pamela Weathers
E P Suada, B Jasim and C J Jimtha contributed equally to this work.
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Suada, E.P., Jasim, B., Jimtha, C.J. et al. Phytostimulatory and hardening period-reducing effects of plant-associated bacteria on micropropagated Musa acuminata cv. Grand Naine. In Vitro Cell.Dev.Biol.-Plant 51, 682–687 (2015). https://doi.org/10.1007/s11627-015-9721-x
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DOI: https://doi.org/10.1007/s11627-015-9721-x