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Effects of Iso-osmotic Concentrations of NaCl and Mannitol on some Metabolic Activity in Calluses of Two Salicornia species

  • Plant Physiology
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Abstract

The comparative effects of salinity and drought on activity of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), and polyphenol oxidase (PPO) and contents of protein, proline, hydrogen peroxide, malondialdehyde (MDA), and carotenoids were studied in calluses of Salicornia persica and Salicornia europaea. Calluses were cultured on Murashige and Skoog medium (MS medium) containing iso-osmotic concentrations of NaCl (100, 300, and 600 mM) and mannitol (200, 500, and 1000 mM). Proline content increased in calluses of both species under NaCl- and mannitol-induced stresses. Under both stresses, S. persica accumulated higher amounts of protein as compared to S. europaea. Under both stresses, protein content in S. persica decreased in comparison to control. Antioxidant enzyme activities showed different trends in the two Salicornia species under salt and drought stresses but were higher in S. europaea than in S. persica. Changes of hydrogen peroxide and MDA contents of calluses subjected to salt stress followed a pattern different from that observed under drought stress. MDA content showed similar changes to hydrogen peroxide content. Carotenoid content decreased in both species under both stresses. Overall, at the cellular level, S. europaea calluses were more tolerant to both NaCl- and mannitol-induced stresses than calluses of S. persica.

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Acknowledgments

The financial support of this research was provided by College of Science, University of Tehran. The authors thank Dr. H. Akhani for providing the seeds of S. persica.

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  1. Department of Plant Sciences, School of Biology, College of Science, University of Tehran, Tehran, 14155, Iran

    Somayeh Torabi & Vahid Niknam

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  1. Somayeh Torabi
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  2. Vahid Niknam
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Correspondence to Vahid Niknam.

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Editor: John Finer

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Torabi, S., Niknam, V. Effects of Iso-osmotic Concentrations of NaCl and Mannitol on some Metabolic Activity in Calluses of Two Salicornia species. In Vitro Cell.Dev.Biol.-Plant 47, 734–742 (2011). https://doi.org/10.1007/s11627-011-9371-6

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