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In vitro induction and identification of autotetraploids of Dioscorea zingiberensis

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Abstract

Dioscorea zingiberensis is an important medicinal plant and a source of diosgenin in China. We report research on the induction, characteristics, and chemical assays of polyploid plants of D. zingiberensis. Immersing calli in 0.3% colchicine solution for 16 h prior to culture induced a high number of autotetraploid plants. The induction rate reached as high as 36.7% of treated calli. More than 50 lines of autotetraploid plants were obtained. All tetraploid plants showed typical polyploidy characteristics. Twenty selected tetraploid lines were transferred to the field for determination of morphological characteristics and for chemical assays. Six elite lines have been selected for further selection and breeding into new varieties for commercial production.

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Acknowledgment

This study was carried out with the financial assistance of Science and Technology Bureau of Jiangsu, P.R. China.

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Authors and Affiliations

  1. Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, Jiangsu, 210038, People’s Republic of China

    Huang Heping, Gao Shanlin, Chen Lanlan & Jiao Xiaoke

  2. School of Traditional Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei, Anhui, 230038, People’s Republic of China

    Huang Heping

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  1. Huang Heping
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  2. Gao Shanlin
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  3. Chen Lanlan
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  4. Jiao Xiaoke
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Correspondence to Gao Shanlin.

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Editor: A. Altman

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Heping, H., Shanlin, G., Lanlan, C. et al. In vitro induction and identification of autotetraploids of Dioscorea zingiberensis . In Vitro Cell.Dev.Biol.-Plant 44, 448–455 (2008). https://doi.org/10.1007/s11627-008-9177-3

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  • DOI: https://doi.org/10.1007/s11627-008-9177-3

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