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Micropropagation of Searsia dentata

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Abstract

A method for in vitro regeneration of Searsia dentata from nodal and shoot tip explants derived from mature trees is outlined. Nodal explants produced multiple shoots from the axis when cultured on Murashige and Skoog (MS) medium containing 3% sucrose supplemented with 0, 5, 7.5, 10, or 12.5 μM N 6-benzyladenine (BA). An average of 5.3 shoots was obtained from nodal explants on 10 μM BA. For shoot tip explants, however, supplementation of α-naphthaleneacetic acid (NAA) with BA favored a caulogenic response. A maximum of 6.1 shoots were produced per shoot tip explant on MS containing 7.5 μM BA plus 5.0 μM NAA. The in vitro-regenerated shoots produced roots when transferred to full-strength MS medium containing 3% sucrose and 10 μM indole-3-butyric acid (IBA). The developed plantlets were transferred initially to a mist house. After an initial acclimatization period of 3–4 mo, plantlets were shifted to the greenhouse where they thrived for 9 mo. The standardized protocol for mass propagation of S. dentata should eliminate the dependence on natural stands of plants for traditional medicinal purposes, and will also serve as a means of conservation as the species is heavily overexploited.

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Figure 1.

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Abbreviations

BA:

N 6-benzyladenine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

Kn:

kinetin

MS:

Murashige and Skoog (1962)

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Acknowledgment

Financial assistance was provided by the National Research Foundation (NRF), South Africa.

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Authors and Affiliations

  1. Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa

    Surya Prakash & Johannes Van Staden

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  1. Surya Prakash
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  2. Johannes Van Staden
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Correspondence to Johannes Van Staden.

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Editor: Johannes van Staden

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Prakash, S., Van Staden, J. Micropropagation of Searsia dentata . In Vitro Cell.Dev.Biol.-Plant 44, 338–341 (2008). https://doi.org/10.1007/s11627-008-9129-y

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