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Co-transformation of gene expression cassettes via particle bombardment to generate safe transgenic plant without any unwanted DNA

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Abstract

The presence of resistant selectable marker genes and other added DNAs such as the vector backbone sequence in transgenic plant might be an unpredictable hazard to the ecosystem as well as to human health, which have affected the safe assessment of transgenic plants seriously. Using minimal gene expression cassette (containing the promoter, coding region, and terminator) without vector backbone sequence for particle bombardment is the new trend of plant genetic transformation. In the present paper, we co-transformed the selectable marker bar gene cassette and non-selected cecropinB gene cassette into rice (Oryza sativa L.) by particle bombardment, then eliminated the selectable marker bar gene in R1 generation applying the hereditary segregation strategy and attained two safe transgenic plants only harboring cecropinB gene cassettes without any superfluous DNA. This is the fist report indicating that the combination of minimal gene cassettes transformation with the co-transformation and segregation strategy can generate selectable marker-free transgenic plants, which will promote the advancement in plant genetic engineering greatly.

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Acknowledgments

This project was carried out in the State Key Laboratory of Rice Biology in China National Rice Research Institute and supported by the grants from the National Nature Science Foundation of China (Nos. 30425034 and 30370132).

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Authors and Affiliations

  1. College of Food, Biological and Environmental Engineering, Zhejiang Gongshang University, Hangzhou, 310035, China

    Yan Zhao

  2. State Key Laboratory of Rice Biology, China National Rice Research Institute, No.359, Tiyuchang Road, Hangzhou City, Zhejiang Province, 310006, People’s Republic of China

    Qian Qian & Da-Nian Huang

  3. Life Science Department of Hangzhou Teacher’s College, Hangzhou, 310036, China

    Hui-Zhong Wang

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  1. Yan Zhao
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  2. Qian Qian
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  3. Hui-Zhong Wang
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  4. Da-Nian Huang
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Correspondence to Qian Qian.

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Editor: D. Dutis

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Zhao, Y., Qian, Q., Wang, HZ. et al. Co-transformation of gene expression cassettes via particle bombardment to generate safe transgenic plant without any unwanted DNA. In Vitro Cell.Dev.Biol.-Plant 43, 328–334 (2007). https://doi.org/10.1007/s11627-007-9051-8

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