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adventitious shoot regeneration from petiole explants of Heracleum candicans wall

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Summary

A method for adventitious shoot induction from petiole explants of Heracleum candicans is reported. Shoot buds were induced on Murashige and Skoog (MS) medium with 4.4μM 6-benzylaminopurine (BA) and 1.1 μM 2,4-dichlorophen-oxyacetic acid (2,4-D). A wound response in the presence of BA and 2,4-D at the time of culture was necessary for inducing shoot buds. The shoot bud regeneration was significantly influenced by size, type and orientation of explants on the culture medium. These shoot buds developed into 4–5 cm shoots upon transfer to a medium containing 1.1μM BA and 0.5 μM α-naphthaleneacetic acid (NAA). The regenerated shoots formed rooted plantlets on MS medium supplemented with 4.9 μM indole-3-butyric acid (IBA). About 15 plants were established in the field for further evaluation.

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Authors and Affiliations

  1. Department of Botany, University of Jammu, 180 006, Jammu, India

    R. K. Sharma & A. K. Wakhlu

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  1. R. K. Sharma
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  2. A. K. Wakhlu
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Sharma, R.K., Wakhlu, A.K. adventitious shoot regeneration from petiole explants of Heracleum candicans wall. In Vitro Cell.Dev.Biol.-Plant 37, 794–797 (2001). https://doi.org/10.1007/s11627-001-0131-x

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  • DOI: https://doi.org/10.1007/s11627-001-0131-x

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