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Determination of Fipronil toxicity by different biomarkers in gill and liver tissue of rainbow trout (Oncorhynchus mykiss)

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Abstract

Insecticides that disrupt the healthy functioning of the ecosystem have toxic potential on non-target organisms. Fish, an important component of the aquatic ecosystem, are exposed to these pesticides in different ways. The stress response is regarded as an adaptive mechanism that allows the fish to cope with the perceived stressor to maintain its normal or homeostatic state. This mechanism is determined by antioxidant parameters and oxidative stress indicators measured in gill and liver tissue of rainbow trout. Accordingly, the effects of fipronil (FP) insecticide on the physiology of rainbow trout (Oncorhynchus mykiss) were determined with using the different biomarkers (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), malondialdehyde (MDA), paraoxonase (PON), arylesterase (ARE), myeloperoxidase (MPO), 8-hydroxy-2-deoxyguanosine (8-OHdG)), and caspase 3 activity) in this study. Different doses of FP inhibited antioxidant enzyme activities in rainbow trout liver and gill tissues while inducing oxidative stress parameter (MDA, MPO, and 8-OHdG) levels. Also, caspase-3 activity was increased in liver and gill tissue, but this increase was statistically significant only in gill tissue (p < 0.05). When the results of the study were taken into consideration, it was concluded that different doses of FP insecticide caused physiological changes in rainbow trout and the studied parameters were usable biomarkers in explaining the adaptive response of stress factor.

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Acknowledgments

We thank for all authors for assistance this research and Application Centre and the Laboratories of the Atatürk University Fisheries Faculty.

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Authors and Affiliations

  1. Department of Aquaculture, Faculty of Fisheries, Ataturk University, Erzurum, Turkey

    Arzu Uçar, Gonca Alak & Muhammed Atamanalp

  2. Department of Basic Sciences, Faculty of Fisheries, Ataturk University, Erzurum, Turkey

    Veysel Parlak

  3. Department of Nutrition and Dietetics, Faculty of Health Sciences, Ataturk University, Erzurum, Turkey

    Fatma Betül Özgeriş

  4. Department of Chemistry, Faculty of Science, University of Yüzüncü Yıl, Van, Turkey

    Aslı Çilingir Yeltekin

Authors
  1. Arzu Uçar
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  2. Veysel Parlak
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  3. Fatma Betül Özgeriş
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  4. Aslı Çilingir Yeltekin
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  5. Gonca Alak
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  6. Muhammed Atamanalp
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Correspondence to Arzu Uçar.

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Conflict of interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All conditions (stocking density, water parameters etc.) were designed according to the welfare-related assessments, suitable for O. mykiss.

Ethics committee

The trials with the rainbow trout were approved by Ataturk University Local Ethical Committee for Animal Studies (approved in 27/02/2020, Acceptance No:13).

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Editor: Tetsuji Okamoto

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Uçar, A., Parlak, V., Özgeriş, F.B. et al. Determination of Fipronil toxicity by different biomarkers in gill and liver tissue of rainbow trout (Oncorhynchus mykiss). In Vitro Cell.Dev.Biol.-Animal 56, 543–549 (2020). https://doi.org/10.1007/s11626-020-00480-3

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