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Effect of EGF and FGF on the expansion properties of human umbilical cord mesenchymal cells

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Abstract

Mesenchymal stem cells have been increasingly introduced to have great potential in regenerative medicine, immunotherapy, and gene therapy due to their unique properties of self-renewal and differentiation into multiple cell lineages. Studies have shown that these properties may be limited and changed by senescence-associated growth arrest under different culture conditions. This study aimed to present the ability of some growth factors on human umbilical cord mesenchymal (hUCM) cells expansion and telomerase activity. To optimize hUCM cell growth, epidermal growth factor (EGF) and fibroblast growth factor (FGF) were utilized in culture media, and the ability of these growth factors on the expression of the telomerase reverse transcriptase (TERT) gene and cell cycle phases was investigated. TERT mRNA expression increased in the hUCM cells treated by EGF and FGF. So, the untreated hUCM cells expressed 30.49 ± 7.15% of TERT, while EGF-treated cells expressed 51.82 ± 12.96% and FGF-treated cells expressed 33.77 ± 11.55% of TERT. Exposure of hUCM cells to EGF or FGF also promoted the progression of cells from G1 to S phase of the cell cycle and induced them to decrease the number of cells entering the G2/M phase. Our study showed that EGF and, to a lesser extent, FGF amplify the proliferation and expansion of hUCM cells.

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Authors and Affiliations

  1. Institute of Bioscience, Universiti Putra Malaysia, Kuala Lumpur, Malaysia

    Parvin Salehinejad

  2. Faculty of Molecular and Biotechnology, Universiti Putra Malaysia, Kuala Lumpur, Malaysia

    Noorjahan Banu Alitheen

  3. Neuroscience research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

    Parvin Salehinejad, Ali Mandegary & Seyed Noureddin Nematollahi-mahani

  4. Department of Toxicology and Pharmacology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

    Ali Mandegary

  5. Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

    Seyed Noureddin Nematollahi-mahani

  6. Stem Cells and Developmental Biology Group of Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

    Ehsan Janzamin

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  1. Parvin Salehinejad
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  2. Noorjahan Banu Alitheen
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  3. Ali Mandegary
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  4. Seyed Noureddin Nematollahi-mahani
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  5. Ehsan Janzamin
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Correspondence to Ali Mandegary.

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Editor: T. Okamoto

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Salehinejad, P., Alitheen, N.B., Mandegary, A. et al. Effect of EGF and FGF on the expansion properties of human umbilical cord mesenchymal cells. In Vitro Cell.Dev.Biol.-Animal 49, 515–523 (2013). https://doi.org/10.1007/s11626-013-9631-3

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  • DOI: https://doi.org/10.1007/s11626-013-9631-3

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