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Tissue-engineered fetal dermal matrices

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Abstract

In the early to mid-gestation fetus, skin wounds heal with no scar formation and perfect restoration of dermal architecture. This phenomenon is intrinsic to fetal skin. The intrinsic phenotypic properties of the fetal fibroblast are believed to be “the effector of scarless repair”. We sought to prepare dermal matrices with high similarity to the mid-gestation fetal dermis using the technology of “self-assembly” with fetal dermal cells of 18, 20, and 22 wk gestation. Comparison of these dermal constructs to those prepared with neonatal dermal cells, adult skin, neonatal foreskin, and mid-gestation fetal skin demonstrates that these fetal dermal matrices bear marked morphological and biochemical resemblance to the mid-gestation fetal dermis. In order to shed further light on the genes involved in scarless wound healing, we conducted a differential gene array analysis of the neonatal and fetal dermal matrices. Using a gene chip (GLYCOv4 gene chip) of approximately 1,260 human genes, we observed differential expression of 67 genes. A number of fibrotic genes were observed to be downregulated and anti-fibrotic genes upregulated.

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Acknowledgments

We thank Maria Ericsson and Louise Trakimas (Harvard Medical School) for their valuable assistance with transmission electron microscopy. We are grateful to Gilberto Hernandez (Scripps Research Institute) for his assistance with RNA analysis. This research was supported by a grant from the Charles H. Hood Foundation to TP.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Bouvé College of Health Sciences, Northeastern University, Boston, MA, 02115, USA

    Tara Pouyani

  2. DNA Array Core Facility, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA, 92037, USA

    Suzanne Papp & Lana Schaffer

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  1. Tara Pouyani
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  2. Suzanne Papp
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  3. Lana Schaffer
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Correspondence to Tara Pouyani.

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Editor: Tetsuji Okamoto

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Pouyani, T., Papp, S. & Schaffer, L. Tissue-engineered fetal dermal matrices. In Vitro Cell.Dev.Biol.-Animal 48, 493–506 (2012). https://doi.org/10.1007/s11626-012-9541-9

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