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Establishment and characterization of a lactating dairy goat mammary gland epithelial cell line

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Abstract

To study milk synthesis in dairy goat mammary gland, we had established an in vitro lactating dairy goat mammary epithelial cell (DGMEC) line. Mammary tissues of Guan Zhong dairy goats at 35 d of lactation were dispersed and cultured in a medium containing epithelial growth factor, insulin-like growth factor-1, insulin transferrin serum, and fetal bovine serum. Epithelial cells were enriched by digesting with 0.25% trypsin repeatedly to remove fibroblast cells and were identified as epithelial origin by staining with antibody against cytokeratine 18. The DGMECs displayed monolayer, cobble-stone, epithelial-like morphology, and formed alveoli-like structures and island monolayer aggregates which were the typical characteristics of mammary epithelial cells. A one-half logarithmically growth curve and cytoplasmic lipid droplets in these cells were observed. In this paper, we also studied the lactating function of DGMECs. Results showed that DGMECs could secrete lactose and β-casein. Lactating function of the cells had no obvious change after 48 h treated by insulin, while prolactin could obviously raise the secretion of milk proteins and lactose.

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Acknowledgment

This work was founded by National Key Basic Research Program of China (project no. 2011CB100804).

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Authors and Affiliations

  1. The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Mucai Street 59, Xiangfang District, Harbin, 150030, Heilongjiang, China

    Hui-Li Tong, Qing-Zhang Li, Xue-Jun Gao & De-Yun Yin

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  1. Hui-Li Tong
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  2. Qing-Zhang Li
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  3. Xue-Jun Gao
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  4. De-Yun Yin
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Correspondence to Xue-Jun Gao.

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Editor: T. Okamoto

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Tong, HL., Li, QZ., Gao, XJ. et al. Establishment and characterization of a lactating dairy goat mammary gland epithelial cell line. In Vitro Cell.Dev.Biol.-Animal 48, 149–155 (2012). https://doi.org/10.1007/s11626-012-9481-4

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  • DOI: https://doi.org/10.1007/s11626-012-9481-4

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