Abstract
Human embryonic stem cell (hESC) lines can be established from the preimplantation embryos. Due to their ability to differentiate into all three embryonic layers, hESC are of significant interest as a renewable source of cell material for different applications, especially for cell replacement therapy. Since the establishment of the first hESC lines in 1998, several studies have described the derivation and culture of new hESC lines using various derivation methods and culture conditions. Our group has currently established eight new hESC lines of which three of the latest ones are described in a more detailed way in this report. The described lines have been established using mechanical derivation methods for surplus bad quality embryos and culture conditions containing human foreskin fibroblast feeder cells and serum-free culture medium. All the new lines have a normal karyotype and typical hESC characteristics analyzed in vitro. The described hESC lines are available for research purposes upon request (www.regea.fi).
Similar content being viewed by others
References
Hoffman L. M.; Carpenter M. K. Characterization and culture of human embryonic stem cells. Nat Biotechnol 23: 699–708; 2005.
Skottman H.; Narkilahti S.; Hovatta O. Challenges and approaches to the culture of pluripotent human embryonic stem cells. Regen Med 2: 265–273; 2007.
Ström S.; Inzunza J.; Grinnemo K. H.; Holmberg K.; Matilainen E.; Strömberg A. M.; Blennow E.; Hovatta O. Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines. Hum Reprod 22: 3051–3058; 2007.
Thomson J. A.; Itskovitz-Eldor J.; Shapiro S. S.; Waknitz M. A.; Swiergiel J. J.; Marshall V. S.; Jones J. M. Embryonic stem cell lines derived from human blastocysts. Science 282: 1145–1147; 1998.
Reubinoff B. E.; Pera M. F.; Vajta G.; Trounson A. O. Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification method. Hum Reprod 16: 2187–2194; 2001.
Acknowledgments
The hESC lines were cultured and characterized by Outi Melin, Hanna Koskenaho, Niina Ikonen, and Sari Leinonen. We also thank other Regea personnel for their support and especially Professor Riitta Suuronen. The personnel of the Tampere University Hospital’s Hormone and Infertility Clinic, Department of Obstetrics and Gynaecology Turku University Hospital and the tissue coordinators of Regea clinical tissue bank are acknowledged for their support in embryo donation procedures, Mrs. Jonna Rinne for revising the language and Ms. Heidi Hakala for assistance with figures.
Author information
Authors and Affiliations
Corresponding author
Additional information
Editor: P. Andrews
Rights and permissions
About this article
Cite this article
Skottman, H. Derivation and characterization of three new human embryonic stem cell lines in Finland. In Vitro Cell.Dev.Biol.-Animal 46, 206–209 (2010). https://doi.org/10.1007/s11626-010-9286-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11626-010-9286-2