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Human Embryonic Stem Cell Line Derivation

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Working with Stem Cells

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Abstract

Human embryonic stem cells are commonly derived from the inner cell mass of developing blastocyst-stage embryos. They are capable of unlimited expansion in vitro and can be maintained in culture indefinitely in their undifferentiated state. These cells can also spontaneously differentiate into different cell types that are representative of the three germ layers (ectoderm, mesoderm and endoderm) both in vitro and in vivo, by generating teratomas after injection into immunocompromised mice. The capacity to differentiate into a variety of cells gives them a promising applicability in cell replacement therapies, and makes hESCs powerful tools for studying the molecular mechanisms underlying cellular differentiation. Here, we will describe protocols to derive hESC lines using conventional procedures and in a xeno-free culture condition, as well as discuss the potential therapeutic use of these cells in regenerative medicine and in pharmaceutical drug screening.

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Authors and Affiliations

  1. National Laboratory of Embryonic Stem Cell (LaNCE), Department of Genetics and Evolutionary Biology, University of São Paulo, Rua do Matão, 277 – sala 350, São Paulo, SP, 05508-090, Brazil

    Simone Aparecida Siqueira Fonseca, Roberta Montero Costas & Lygia V. Pereira

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  1. Simone Aparecida Siqueira Fonseca
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  2. Roberta Montero Costas
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  3. Lygia V. Pereira
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Correspondence to Lygia V. Pereira .

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Fonseca, S.A.S., Costas, R.M., Pereira, L.V. (2016). Human Embryonic Stem Cell Line Derivation. In: Working with Stem Cells. Springer, Cham. https://doi.org/10.1007/978-3-319-30582-0_2

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