Abstract
In this report, we present the derivation and characterization of 15 hESC lines established at the Vrije Universiteit Brussel, Belgium in collaboration with the Universitair Ziekenhuis Brussel, Belgium, using surplus in vitro fertilization embryos and embryos carrying monogenic disorders donated for research. Four lines were derived from blastocyst-stage embryos presumed to be genetically normal, and 11 hESC lines were obtained from embryos shown to carry genetic mutations by preimplantation genetic diagnosis. All the lines express markers of pluripotency as determined by immunocytochemistry and RT-PCR, and formed teratomas when injected into SCID mice. All VUB hESC lines, except for VUB17, are reported in the European hESC registry and are available upon request after signing a Material Transfer Agreement from the VUB (contact person: Prof. Dr. Karen Sermon; Karen.Sermon@uzbrussel.be).
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Acknowledgments
We gratefully acknowledge the assistance of Mieke Geens, Lindsey Van Haute, Bing Chen, and Anna Seriola Petit for maintaining the hESC lines in culture. The authors are also grateful to Debby Van Boxstael for performing part of the genetic analysis of the hESC lines. Ileana Mateizel is supported by the Research Council (OZR) of the Vrije Universiteit Brussel and the FWO (Fonds voor Wetenschappelijk Onderzoek, Vlaanderen, Fund for Scientific Research Flanders). Claudia Spits is a postdoctoral fellow of the FWO.
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Editor: P. Andrews
Ileana Mateizel and Claudia Spits contributed equally to this work.
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Mateizel, I., Spits, C., De Rycke, M. et al. Derivation, culture, and characterization of VUB hESC lines. In Vitro Cell.Dev.Biol.-Animal 46, 300–308 (2010). https://doi.org/10.1007/s11626-010-9284-4
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DOI: https://doi.org/10.1007/s11626-010-9284-4